OsCYP21-4, a novel Golgi-resident cyclophilin, increases oxidative stress tolerance in rice

Swertia mussotii is an important medicinal plant found on the Qinghai Tibetan Plateau that has great economic and medicinal value. This plant has enjoyed a long history of use as a curative for hepatitis. The biological activity of secoiridoids, including gentiopicroside and swertiamarin, has been mainly tested for its anti-hepatitis effects. Here, we identify two candidate genes (SmIS1 and SmIS2) that are homologues of iridoid synthase and that are components of the secoiridoid pathway in S. mussotii. Using sequencing and phylogenetic analyses, we confirm that SmIS1 and SmIS2 contain six conserved short-chain dehydrogenases/reductase (SDR) motifs and thus belong to the P5βRs group. The two purified Escherichia coli-expressed proteins reduced 8-oxogeranial to both nepetalactol and iridodials. A comparison of the kinetic parameters of SmIS1 and SmIS2 recombinant proteins revealed that SmIS2 has a lower affinity than SmIS1 for 8-oxogeranial. Transcript levels of the two genes were analysed in three different tissues of S. mussotii using semi-quantitative RT-PCR and RT-qPCR. SmIS1 and SmIS2 expression levels were more abundant in leaves and stems. This investigation adds to our knowledge of P5βRs genes in the secoiridoid synthesis pathway and provides candidate genes for genetically improving S. mussotii by enhancing secondary metabolite production.

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“…We predicted the subcellular localization of SmIS1 and SmIS2 using WOLF-PSORT tool ( ), Predotar ( ), and ProtComp ( ) [ 31 , 32 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

Cloning and Characterization of Two Iridoid Synthase Homologs from Swertia Mussotii

Bai

Wang

et al. 2017

Molecules

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“…Until now, many cyclophilins genes have been discovered in Glycine max, Arabidopsis thaliana , and O . sativa , and they can be induced by a serial of abiotic stress, and heterologous expression confers toward multiple abiotic stresses ( Mainali et al, 2014 ; Vasudevan et al, 2014 ; Lee et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa

et al. 2016

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Peroxisomes are single membrane-bound organelles, whose basic enzymatic constituents are catalase and H2O2-producing flavin oxidases. Previous reports showed that peroxisome is involved in numerous processes including primary and secondary metabolism, plant development and abiotic stress responses. However, knowledge on the function of different peroxisome genes from rice and its regulatory roles in salt and other abiotic stresses is limited. Here, a novel prey protein, OsPEX11 (Os03g0302000), was screened and identified by yeast two-hybrid and GST pull-down assays. Phenotypic analysis of OsPEX11 overexpression seedlings demonstrated that they had better tolerance to salt stress than wild type (WT) and OsPEX11-RNAi seedlings. Compared with WT and OsPEX11-RNAi seedlings, overexpression of OsPEX11 had lower level of lipid peroxidation, Na+/K+ ratio, higher activities of antioxidant enzymes (SOD, POD, and CAT) and proline accumulation. Furthermore, qPCR data suggested that OsPEX11 acted as a positive regulator of salt tolerance by reinforcing the expression of several well-known rice transporters (OsHKT2;1, OsHKT1;5, OsLti6a, OsLti6b, OsSOS1, OsNHX1, and OsAKT1) involved in Na+/K+ homeostasis in transgenic plants under salinity. Ultrastructural observations of OsPEX11-RNAi seedlings showed that they were less sensitive to salt stress than WT and overexpression lines. These results provide experimental evidence that OsPEX11 is an important gene implicated in Na+ and K+ regulation, and plays a critical role in salt stress tolerance by modulating the expression of cation transporters and antioxidant defense. Thus, OsPEX11 could be considered in transgenic breeding for improvement of salt stress tolerance in rice crop.

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“…Still, a potential role of the Golgi in oxidative stress responses in plants was presented only recently (Lee et al, 2015). The trans-Golgi network regulates cell expansion by directing vesicle trafficking and targeting (Gendre et al, 2011).…”

Section: Ros and The Regulation Of The Cytoskeleton And Vesicle Transmentioning

confidence: 99%

Role of Reactive Oxygen Species during Cell Expansion in Leaves

2016

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OsCYP21-4, a novel Golgi-resident cyclophilin, increases oxidative stress tolerance in rice

Cited by 29 publications

References 70 publications

Cloning and Characterization of Two Iridoid Synthase Homologs from Swertia Mussotii

Cloning and Characterization of Two Iridoid Synthase Homologs from Swertia Mussotii

OsPEX11, a Peroxisomal Biogenesis Factor 11, Contributes to Salt Stress Tolerance in Oryza sativa

Role of Reactive Oxygen Species during Cell Expansion in Leaves

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