Oryzihumus terrae sp. nov., isolated from soil and emended description of the genus Oryzihumus

Abstract

A Gram-stain-positive, aerobic, non-flagellated bacterium, designated KIS22-12T, was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. Cells were non-spore-forming cocci showing catalase-positive and oxidase-negative reactions. Growth of strain KIS22-12T was observed between 10 and 35 °C (optimum, 28–30 °C), between pH 5.0 and 9.0 (optimum, pH 7.0) and with 0–3 % (w/v) NaCl. Strain KIS22-12T contained MK-8(H4) as the predominant menaquinone, and C17 : 1ω8c, iso-C15 : 0 and anteiso-C15 : 0 as the major fatty acids. Strain KIS22-12T contained diphosphatidylglycerol, phosphatidylinositol, one unknown aminophospholipid, one unknown aminolipid, two unknown phospholipids and one unknown lipid. The peptidoglycan type was A1γ. The G+C content of the genomic DNA was 75.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KIS22-12T formed a phyletic lineage with Oryzihumus leptocrescens KV-628T. 16S rRNA gene sequence similarity between the two strains was 96.5 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain KIS22-12T represents a novel species within the genus Oryzihumus , for which the name Oryzihumus terrae sp. nov. is proposed. The type strain is KIS22-12T ( = KACC 16543T = DSM 27161T = NBRC 109596T). An emended description of the genus Oryzihumus is also provided.

Oryzobacter terrae gen. nov., sp. nov., isolated from paddy soil

“…nov. Table 1. Differential characteristics of strain PSGM2-1 T and genera of the family Intrasporangiaceae Taxa: 1, PSGM2-1 T (data from this study); 2, Aquipuribacter (Tó th et al, 2012); 3, Arsenicicoccus (Collins et al, 2004); 4, Fodinibacter (Wang et al, 2009); 5, Humibacillus (Kageyama et al, 2008b); 6, Intrasporangium (Kalakoutskii, 1989;Schumann et al, 1997); 7, Janibacter (Martin et al, 1997); 8, Knoellia (Groth et al, 2002); 9, Kribbia (Jung et al, 2006); 10, Lapillicoccus (Lee & Lee, 2007); 11, Marihabitans (Kageyama et al, 2008a); 12, Ornithinibacter (Xiao et al, 2011); 13, Ornithinicoccus (Groth et al, 1999); 14, Ornithinimicrobium (Groth et al, 2001); 15, Oryzihumus (Kageyama et al, 2005;Lim et al, 2014); 16, Phycicoccus (Lee, 2006); 17, Serinicoccus (Yi et al, 2004); 18, Terrabacter (Collins et al, 1989;Weon et al, 2010); 19, Terracoccus (Prauser et al, 1997); 20, Tetrasphaera (Maszenan et al, 2000). +, Positive; 2, negative; ND, not determined.…”