Oryzobacter terrae gen. nov., sp. nov., isolated from paddy soil

Kim, Soojin; Lim, Jun-Muk; Hamada, Moriyuki; Ahn, Jae-Hyung; Weon, Hang-Yeon; Suzuki, Ken‐ichiro; Ahn, Tae‐Young; Kwon, Soon-Wo

doi:10.1099/ijsem.0.000398

Cited by 12 publications

(2 citation statements)

References 34 publications

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“…The specie Oryzobacter terrae was the only specie of the genus present in the samples. It has been described as a rod-shaped bacterium isolated from rice soil [29]. O. terrae seems to be very sensible to the toxic effect of the insecticide.…”

Section: Genera Variation After the Spillmentioning

confidence: 99%

Microbiome and Respirometry Analysis of the Bacterial Community in a Bench-Scale Activated Sludge Reactor Exposed to Insecticide

Baeza-Serrano¹,

Tárrega²,

Martínez-Blanch³

et al. 2022

Int. J. EI

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Wastewater treatment plants (WWTPs) have to treat sewage 24 h/365 days a year. Uncontrolled toxic spills can affect the microbial community in activated sludge and thus the biological treatment performance. Water below the quality requirements, established in the legislation could be, therefore released to the receiving environment, contributing to the loss of biological diversity, degradation of water resources and generating public health threats.This study aims to analyse the community changes that occurred in an activated sludge as consequence of an insecticide spill in a laboratory-scale reactor. Next-generation sequencing was applied to identify genera that could serve as key indicators of a negative biological process affection.The bench-scale system consisted of a 10-litre biological reactor with intermittent aeration cycles to remove organic matter and nutrients, which was fed with primary settled sewage, follow by a secondary clarifier with manual purging and an external recirculation to the biological reactor. Both settled sewage and mixed liquor were obtained from the same WWTP.The effect of an artificial spill of insecticide containing D-tetramethrin, cyphenothrin and pyriproxyfen on the activated sludge biomass was studied through both, respirometry and microbiome analysis.The toxic effect on the activated sludge community demonstrated to be significant, completely inhibiting nitrifying activity and changing the distribution of the microbiome. Results show that relative abundance of certain groups increased after the spill, being then the new conditions favourable for the development of these groups. By contrast, others groups are shown to be extremely sensitive to the toxic effect, such that their disappearance can act as a key indicator of an insecticide spill occurrence in a WWTP. however, the abundance of nitrifying bacteria was not affected.

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Section: Genera Variation After the Spillmentioning

confidence: 99%

Microbiome and Respirometry Analysis of the Bacterial Community in a Bench-Scale Activated Sludge Reactor Exposed to Insecticide

Baeza-Serrano¹,

Tárrega²,

Martínez-Blanch³

et al. 2022

Int. J. EI

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“…Oryzobacter genera suffer a sudden drop only 2 hours after the spill, almost disappearing in the next days (relative abundance <0.016 in day 7). This genus was represented in the samples by the only specie Oryzobacter terrae, which is a rod-shaped bacterium isolated from rice soil [29]. This specie seems to be very sensible to the spill effect.…”

Section: Genera Variation After the Spillmentioning

confidence: 99%

Microbiome Analysis of the Bacterial Population in a Bench-Scale-Activated Sludge Reactor Exposed to an Artificial Insecticide Spill

Baeza-Serrano¹,

Tárrega

Martínez-Blanch³

et al. 2020

WIT Transactions on Ecology and the Environment

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Wastewater treatment plants (WWTPs) must process wastewater efficient and continuously. Uncontrolled toxic spills can damage the activated sludge units, reducing the treatment capacity of the bacterial culture. Consequently, water below the quality requirements could be released to the receiving environment, contributing to the loss of biological diversity, degradation of water resources and generating public health threats. The objective of this assay was to study the changes that an insecticide spill produces in the activated sludge microbiome of a laboratory-scale reactor. Next-generation sequencing was carried out to idntify species that could serve as key indicators of a negative biological process affection. The bench-scale system consisted of a vessel with primary settled wastewater feeding a 10-L biological reactor with intermittent aeration cycles to remove organic matter and nutrients. A secondary clarifier with manual purging and an external recirculation to the biological reactor completed the system. Both settled wastewater and activated sludge was obtained from the same WWTP. An insecticide containing D-tetramethrin, cyphenothrin and pyriproxyfen was previously proven to decrease significantly the nitrification activity of activated sludge with a 2 mL dose. The experiment was repeated with biological sampling to monitor activated sludge microbiome 2 hours after the spill, and on days 1, 2, 3, 6 and 7. The insecticide's toxic effect on the biological process was demonstrated to be significant, changing the distribution of the activated sludge population. Results show that relative abundance of certain groups increased 2 hours after the spill, with one species specifically being very sensitive to the insecticide effect, disappearing completely after only 2 hours. These results show that some microorganism groups could be monitored as indicators of a negative affection in the activated sludge biological process and have a potential use to develop early detection kits for biological failures in WWTPs.

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Marihabitans

Kasai

Takahashi

2021

Bergey's Manual of Systematics of Archaea and Bacteria

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Ma.ri.ha'bi.tans. L. neut. n. mare sea; L. part. adj. habitans inhabiting; N.L. adj. used as a neut. subst. Marihabitans inhabitant of the sea. “Actinobacteria” / Actinobacteria / Micrococcales / Intrasporangiaceae / Marihabitans Cells are Gram‐stain‐positive, nonmotile, non‐spore‐forming, catalase‐positive, aerobic, and irregular short rods and cocci isolated from surface seawater, deep seawater, and marine sponges, which are 0.9–1.4 μm wide and 1.2–1.7 μm long. The peptidoglycan is of the A type and contains meso ‐A 2 pm, alanine, and glutamic acid. The cell‐wall sugars are galactose, glucose, and ribose. Mycolic acids are absent. The major menaquinone is MK‐8(H 4 ). The phospholipids comprise phosphatidylglycerol, an unknown phospholipid, diphosphatidylglycerol, and phosphatidylinositol mannosides. Phylogenetically, the genus is a member of the family Intrasporangiaceae . Most closely related taxon is the genus Kribbia in the 16S rRNA gene‐based phylogenetic tree. The similarity value of the 16S rRNA gene sequence is 96.3% between the type species, Marihabitans asiaticum HG667 T and Kribbia dieselivorans N113 T . Marihabitans is distinguishable from Kribbia by the absence of saturated fatty acids in the predominant fatty acid profile. The genome sequence of the type species is 2,953,873 bp, and 2,694 protein‐coding genes are predicted. The calculated G + C content is 71.1%. DNA G + C content (mol%) : 71.1% (genome sequence); 70 (HPLC). Type species : Marihabitans asiaticum Kageyama et al. 2008 VP .

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Oryzobacter terrae gen. nov., sp. nov., isolated from paddy soil

Cited by 12 publications

References 34 publications

Microbiome and Respirometry Analysis of the Bacterial Community in a Bench-Scale Activated Sludge Reactor Exposed to Insecticide

Microbiome and Respirometry Analysis of the Bacterial Community in a Bench-Scale Activated Sludge Reactor Exposed to Insecticide

Microbiome Analysis of the Bacterial Population in a Bench-Scale-Activated Sludge Reactor Exposed to an Artificial Insecticide Spill

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