Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability

Kermani, Maryam Jafarkhani; Sarasan, Viswambharan; Roberts, A.; Yokoya, K.; Wentworth, Jonathan; Sieber, Vivien

doi:10.1007/s00122-003-1374-1

Cited by 131 publications

(110 citation statements)

References 9 publications

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“…leaf colour and anatomy in Citrus spp. (Allario et al 2011), leaf anatomy in Lonicera ), cell anatomy in Lolium (Sugiyama 2005) and leaf morphology and colour in Rosa (Kermani et al 2003). The variation in leaf colour in our study could be significantly correlated with the pigment concentration per mm² leaf area.…”

Section: Discussionsupporting

confidence: 56%

“…Morphological effects commonly associated with polyploidy are bigger flowers, an altered leaf length-to-width ratio and changes in size and density of stomata (Sun et al 2009;Van Laere et al 2011;Trojak-Goluch, Skomra 2013;Tan et al 2015). Other frequently occurring characteristics include darker-coloured green leaves and a more compact growth habit (Kermani et al 2003;Liu et al 2007;Allario et al 2011).…”

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confidence: 99%

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Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Hias¹,

Leus²,

Davey³

et al. 2017

Hortic. Sci.

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Hias N., Leus L., Davey M.W., Vanderzande S., Van Huylenbroeck J., Keulemans J. (2017): Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes. Hort. Sci. (Prague), 44: 55-63.Because polyploidy often results in enhancement of desirable properties, artificial genome doubling is commonly used in agri-and horticultural crop breeding programs. In this study genome doubling was induced in two apple genotypes. The effect on vegetative morphological and physiological traits of the plants was then comprehensively determined by comparing the obtained tetraploid apple plants with their diploid counterparts. Out of 17 different physio-and morphological characteristics, 15 were significantly affected in one or both genotypes. The response of these 15 characteristics also appeared to have been caused by two effects; 10 of the 15 characteristics exhibited a common response to ploidy change over both genotypes while five traits showed a genotype-specific response to polyploidization. Tetraploid leaves also exhibited a darker leaf colour, which could be correlated to a higher pigment concentration. Furthermore, the results also show a decreased elongation rate and leaf size in tetraploids, which is suggested to be due to the observed lower cell density in the polyploid apple plants.

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Section: Discussionsupporting

confidence: 56%

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confidence: 99%

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Hias¹,

Leus²,

Davey³

et al. 2017

Hortic. Sci.

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“…The same results were reported by Lin et al (2010) on in vitro polyploids E. globulus plants which had thicker, wider and darker green leaves. Similarly, the polyploid plants of rose showed thicker and darker green leaves than the control diploid plants (Kermani, Sarasan, Roberts, Yokoya, Wentworth, & Sieber, 2003). Similary of Griffin (2014), tetraploids A. mangium produced gigantism effect.…”

Section: Change Of Plantlets Morphologysupporting

confidence: 65%

GROWTH AND MORPHOLOGICAL CHANGES AS AN EARLY INDICATION OF IN VITRO PLOIDIZATION OF TEAK (Tectona grandis L.f)

Fauzan¹,

Supriyanto²,

Tajuddin³

2017

Jur.pen.hut.tanaman

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Ploidization level estimation can be conducted in several methods, through morphological, growth response, anatomy, cytology, and molecular markers. The simplest and easiest methods are morphological marker and growth response. The study aimed to develop early detection method of polyploidy occurrence in in vitro Tectona grandis after treated by antimitotic agent colchicine. Nodal segments were immersed at 0, 15, and 30 μM colchicines for 5 days, then cultured for 8 weeks. Observations on plantlet height, number of leaves and morphology were performed at 2, 4, and 6 weeks after planting. Colchicine had high significant effect on the height and significant effect on leaves number. High concentration colchicine inhibited shoot elongation and leaves growth, however it increased morphological changes. The planlets height of 0, 15, and 30 μM of colchicine treatment was 4.14; 3.82; 3.12 cm; while the number of leaves as much as 8.72; 8.4, and 7.5. Colchicine led to increase in morphological changes at the levels 0, 15, 30 μM were 26,60%; 46.66%; and 93.33%. Changes caused by polyploidy differ from media. Changes in polyploidy decreased the height, number of leaves, and induced morphological changes, whereas planting media resulted in vitrification. Response to colchicines in culture of T. grandis plantlet allows the growth and morphology to be a marker for early detection of polyploidization.Key words: Colchicine, polyploidy detection, tectona grandis ABSTRAK Pendugaan tingkat ploidi dapat dilakukan dengan beberapa metode, antara lain: penanda morfologi, respon pertumbuhan, anatomi, sitologi, dan molekuler. Metode yang paling sederhana dan mudah dilakukan, terutama untuk deteksi dini yaitu dengan penanda morfologi dan respon pertumbuhan. Penelitian ini bertujuan untuk mengamati deteksi dini terjadinya poliploidisasi Tectona grandis akibat pemberian kolkisin pada kultur in vitro. Ruas nodus eksplan jati direndam dalam 3 konsentrasi kolsikin (0, 15, dan 30 μM) selama 5 hari, untuk selanjutnya dilakukan kultur in vitro selama 8 minggu. Pengamatan terhadap tinggi daun, jumlah daun dan morfologi daun dilakukan pada minggu ke-2, ke-4, dan ke-6 setelah tanam. Hasil penelitian menunjukkan pemberian kolsikin berpengaruh sangat nyata terhadap tinggi dan berpengaruh nyata terhadap jumlah daun. Pertumbuhan tinggi daun dan jumlah daun mengalami penurunan seiring dengan bertambahnya konsentrasi kolkisin. Tinggi planlet akibat perlakuan perendaman 0, 15, dan 30 μM kolkisin adalah 4,14; 3,82; dan 3,12 cm; sedangkan jumlah daun sebanyak 8,72; 8,4; dan 7,5. Peningkatan konsentrasi kolkisin menyebabkan peningkatan perubahan morfologi. Perubahan morfologi perlakuan kontrol, 15 dan 30 μM kolkisin sebesar 26,60%, 46,66%, dan 93,33%. Perubahan karena poliploidi berbeda dengan perubahan karena media. Perubahan akibat poliploidi menyebabkan perubahan pada tinggi planlet, jumlah daun, serta morfologi; sedangkan perubahan media tanam menyebabkan vitrifikasi. Adanya respon pemberian kolkisin pada kultur in vitro T. grandis memungkinkan p...

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“…Oryzalin, a microtubule inhibitor, blocks microtubule assembly but not ongoing disassembly, causing microtubule depolymerization. Oryzalin is used to induce chromosome doubling in plants by inhibiting mitotic activity and is considered a preferable alternative to colchicine because of its lower toxicity (Kermani et al, 2003;Dhooghe et al, 2011;Rêgo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of Enhanced Ultraviolet-B Radiation on Chromosomes and Microtubule Arrays in Wheat Roots

Chen¹,

Chen²,

Han³

2016

IJAB

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To cite this paper: Chen, P.X., H. Chen and R. Han, 2016 AbstractEnhanced ultraviolet-B (UV-B) radiation causes chromosomal aberrations and abnormal microtubule arrays in wheat (Triticum aestivum) root cells. Oryzalin induces chromosome doubling through depolymerization of microtubules and results in diploidization. We explored the effect of oryzalin on wheat roots exposed to enhanced UV-B radiation. Treatment with 500 µmol·L −1 oryzalin resulted in chromosome doubling with two equal nuclei. However, exposure to UV-B radiation in combination with oryzalin resulted in two unequal nuclei. The frequency of unequal nuclei caused by treatment with UV-B and oryzalin was higher than that caused by UV-B exposure alone. During the mitotic phase, chromosomal aberrations were indicated to be independent of abnormal microtubule structures. These suggested that chromosomal aberrations caused by enhanced UV-B radiation were not a consequence of abnormal microtubule structures.

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Oryzalin-induced chromosome doubling in Rosa and its effect on plant morphology and pollen viability

Cited by 131 publications

References 9 publications

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

GROWTH AND MORPHOLOGICAL CHANGES AS AN EARLY INDICATION OF IN VITRO PLOIDIZATION OF TEAK (Tectona grandis L.f)

Effects of Enhanced Ultraviolet-B Radiation on Chromosomes and Microtubule Arrays in Wheat Roots

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