Orthotopic Ovarian Transplantation in Mice

Jones, Emma R.; Krohn, P. L.

doi:10.1677/joe.0.0200135

Cited by 89 publications

(41 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another approach to address this issue is the transplantation of whole ovaries from the young mice to the old and vice versa [35,36]. Ovaries from the young mice failed to restore the reproductive output of the old mice, though the oestrous cycles reappeared.…”

Section: Uterine Aging Contributes To Decreased Reproductive Capacitymentioning

confidence: 99%

Determinants of uterine aging: lessons from rodent models

Kong

Zhang

Chen

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

The uterus is an indispensable organ for the development of a new life in eutherian mammals. The female mammalian reproductive capacity diminishes with age. In this respect, the senescence of uterine endometrium is convinced to contribute to this failure. This review focuses on the physiological function of the uterus and the related influence of aging mainly in rodent models. A better understanding of the underlying mechanisms governing the process of uterine aging is hoped to generate new strategies to prolong the reproductive lifespan in humans. . More importantly, this phenomenon of continuous decline of fecundity with age has also been confirmed in women over 35 years old [10][11][12]. The success of reproduction in eutherian mammals requires the uterus, a unique organ in the viviparity compared to other organisms ensuring full development of the new life in the maternal body [13]. The endometrial environment of uteri is proved to be one of the critical aspects to determine the reproductive capacity [14] and uterine factor has been proved to be critical for the decline of fecundity in both rodents and humans [4,15]. Most of the available information on uterine aging derives from studies on rats and mice [16]. In these species, life span is short and aging animal model can be easily established. This review focuses on the physiological function of the uterus and the related influence of aging mainly in rodent models. determinants, uterine aging, rodent models

show abstract

Section: Uterine Aging Contributes To Decreased Reproductive Capacitymentioning

confidence: 99%

Determinants of uterine aging: lessons from rodent models

Kong

Zhang

Chen

et al. 2012

Sci. China Life Sci.

View full text Add to dashboard Cite

show abstract

“…The technique was adapted from Jones and Krohn (1960) who performed orthotopic transplantation of the ovary in the mouse. Recipient females were anaesthetized with a mixture of ketamine (WDT, Garbsen, Germany) and xylazine (Rompun 2%, Bayer, Leverkusen, Germany): 950 m l ketamine 10% plus 50 m l xylazine were mixed.…”

Section: Transplantation Of Ovariesmentioning

confidence: 99%

Orthotopic transplantation of rat ovaries as a tool for strain rescue

Dorsch

Wedekind²,

Kamino³

et al. 2004

Lab Anim

View full text Add to dashboard Cite

SummarySpontaneous mutations in inbred strains provide valuable resources for new disease models. Unfortunately, these mutations may affect reproduction, which require considerable efforts in breeding management. We transplanted ovaries of such mutant rat strains orthotopically into ovariectomized premature coisogenic recipients. A reproductive cycle was established in each of the recipients within 5 to 6 weeks after transplantation. Moreover, one-third became pregnant and had litters an average of 3 months after transplantation. These experiments demonstrate that orthotopic transplantation of ovaries can be used in the management of subfertile rat colonies.

show abstract

“…Orthotopic grafts permit natural conception and circumvent the need for in vitro maturation (IVM) and/or in vitro fertilization (IVF) that is required for heterotopic grafts (Jones & Krohn 1960, Gosden et al 1994, Cox et al 1996, Petroianu et al 2002, Almodin et al 2004a, b, Donnez et al 2004. However, most clinical studies to date have placed ovarian grafts under the skin to permit easy monitoring of follicle growth and provide ready access to the graft for oocyte collection (Oktay & Yih 2002, Oktay et al 2003.…”

Section: Introductionmentioning

confidence: 99%

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Yang¹,

Cox²,

Jenkin³

et al. 2006

Reproduction

View full text Add to dashboard Cite

Ovarian tissue cryopreservation and subsequent transplantation can restore fertility in cancer patients. This study used a mouse ovarian grafting model to investigate whether the graft site (bursal cavity, the kidney capsule or subcutaneous) influences the number, fertilization rate and developmental potential of oocytes recovered from grafts and whether using a standard gonadotrophin stimulation protocol would increase oocyte yield from the grafts. Mouse ovarian tissue was grafted into four week old mice and collected three weeks later. Graft recipients were treated either with or without exogenous gonadotrophin stimulation prior to graft collection. Grafted ovaries yielded oocytes that were either at the germinal vesicle (GV) stage or mature metaphase II (MII) stage at collection. These GV oocytes were matured before in vitro fertilization (IVF), while the MII oocytes underwent IVF immediately. Oocytes collected from the oviducts of non-grafted superovulated mice of the same age served as controls. Two-cell embryos were transferred to pseudopregnant recipients and recovered at day 15 of gestation or left to go to term. Graft retrieval and the number of oocytes from each graft were lowest from the subcutaneous graft site. The number of two-cell embryos produced was significantly higher for oocytes from the grafts to the bursa as compared with the other sites. All graft sites gave rise to embryos with comparable implantation rates and developmental potential to fetuses and offspring following transfer. However, the oocytes from grafted ovaries had a significantly lower developmental potential when compared with the control group. Stimulation with exogenous gonadotrophins did not significantly increase oocyte yield from grafted ovaries but did enhance oocyte maturation and development. In conclusion, graft site affects the number and quality of oocytes produced from ovarian grafts.

show abstract

Orthotopic Ovarian Transplantation in Mice

Cited by 89 publications

References 1 publication

Determinants of uterine aging: lessons from rodent models

Determinants of uterine aging: lessons from rodent models

Orthotopic transplantation of rat ovaries as a tool for strain rescue

Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts

Contact Info

Product

Resources

About