Orthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation

Zhou, Xianfeng; Moussa, Fouad M.; Mankoci, Steven; Ustriyana, Putu; Zhang, Nianli; Abdelmagid, Samir M.; Molenda, Jim; Murphy, William L.; Safadi, Fayez F.; Sahai, Nita

doi:10.1016/j.actbio.2016.05.007

Cited by 69 publications

(59 citation statements)

References 69 publications

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“…1 Osteoblast differentiation is a complicated process, in which many signals and factors participated, 2 including NF-κB, 3 JNK, and p38, 4 as well as BMP2. 1 Osteoblast differentiation is a complicated process, in which many signals and factors participated, 2 including NF-κB, 3 JNK, and p38, 4 as well as BMP2.…”

Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

Zhang

Zheng

Zhu

2018

J of Cellular Biochemistry

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Osteoblasts have the capacity to differentiate into several different cell types, including adipocyte, chondrocyte, and muscle lineages. Therefore, osteoblast can be potentially applied in the treatment of bone diseases. The factors controlling osteoblast differentiation is complex. Recently, it has been reported that some natural products regulate the differentiation in osteoblasts and promote bone formation. Based on these findings, this study demonstrated that Lycium barbarum polysaccharides (LBP) could promote proliferation of osteoblast MC3T3‐E1 cells through 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Besides, expression of key proteins correlated with cellular proliferation such as proliferating cell nuclear antigen (PCNA) and Ki67 was enhanced in the presence of LBP. We also detected the increased expression of bone‐specific matrix proteins such as morphogenetic protein 2 (BMP2), bone Gla protein (BGP), osteopontin (OPN), and α‐1 type‐I collagen (COL1A1) when treated with LBP. This process was mediated by some signals, such as smad1, smad8, Runt‐related transcription factor 2 (RUNX2), and Osterix. Furthermore, RUNX2 silencing inhibited osteoblast differentiation by decreasing expression of bone‐specific matrix proteins. Collectively, we proposed a previously unidentified function of LBP in osteoblast differentiation, suggesting its potential clinical role in bone disease treatment.

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Section: Introductionmentioning

confidence: 99%

Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

Zhang

Zheng

Zhu

2018

J of Cellular Biochemistry

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“…Tsigkou et al (2009) revealed that gene expression in primary osteoblasts derived from the human foetal long bone was also influenced by the presence of extracts and showed dependence on Si concentration in the extract [22]. Some studies suggested that silicate ions enter the cells and modulate cellular functions by affecting gene expression [10,11]. Therefore, we hypothesise that ionic structure defines the efficiency of uptake of silicate ions by the cell.…”

Section: Discussionmentioning

confidence: 99%

“…The aim of the present work was, therefore, to compare the influence of silicate ions released from BG and SiV on osteoblast-like cell functions. Whereas others have investigated the role of BG ions on osteoblast behaviour [5][6][7][8][9][10][11], the differences between Si species has not been studied. This study demonstrates for the first time that Si ion species influence ion uptake and cellular properties of osteoblast-like cells, depending on the stage of osteoblast differentiation and duration of exposure.…”

Section: Introductionmentioning

confidence: 99%

“…Although the mechanisms underlying the regulation of gene expression by bioactive glasses are not fully understood, several possible pathways have been suggested. Zhou et al (2016) reported uptake of orthosilicic acid, Si(OH)4, by human mesenchymal stem cells (hMSCs), which reduced NF-κB and Runt-related transcription factor 2 (RUNX2) activity via the up-regulation of interfering RNA (microRNA-146a) [10]. RUNX2 is the primary transcription factor necessary for osteoblast precursor differentiation.…”

Section: Introductionmentioning

confidence: 99%

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Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite

et al. 2017

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Purpose: Silicate ions released from bioactive glasses and ceramics have been reported to stimulate osteogenic cell functions. Here, we evaluated osteoblast-like cell reactions to silicate ions released from two different types of materials, 45S5 bioactive glass (BG) and siloxane-doped vaterite (SiV), to investigate the influence of the ionic structure of silicate ions on osteoblast-like cell properties.Methods: BG and SiV powders were prepared by using melt-quenching and carbonation methods, respectively. Aminopropyltriethoxysilane was used as a siloxane source of SiV. MC3T3-E1 and SaOS-2 cells were cultured in media containing dissolved BG or SiV ions (10-50 ppm of Si). Cell proliferation (metabolic activity), differentiation (alkaline phosphatase activity) and mineralisation (Ca deposition) were examined. Results:29 Si NMR spectra demonstrated that Q 0,1 species and T 0-3 species were released from BG and SiV, respectively. Proliferation and mineralisation of the two types of cells were influenced by silicate ions released from BG and SiV in a concentration-dependent manner. In particular, there were significant differences (P < 0.05) in the degree of proliferation and Ca deposition levels in SaOS-2 cells treated with dissolved BG and SiV ions. Furthermore, Ca deposition in SaOS-2 cells was influenced by both the presence of silicate ions and the duration of exposure of cells to them. Conclusions:The structure of silicate ions influenced the proliferation and mineralisation of SaOS-2 cells incubated for different time periods in culture media containing different Si concentrations. Understanding the effect of Si on bone cell behaviour will enable a design-led approach to further BG optimisation. KeywordsSilicate ions, 45S5-type bioactive glass, Vaterite, Osteoblast-like cells

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“…Below this concentration, our studies showed that soluble Si(OH) 4 stimulates the proliferation of hMSCs and mHSCs in normal growth medium. More interestingly, we found the Si(OH) 4 regulated bone formation and bone resorption by promoting osteoblastic gene program while repressing osteoclast differentiation . To elucidate the possible molecular mechanisms, differential microRNA microarray analyses were applied.…”

Section: The Controversial Roles Of Silicatementioning

confidence: 99%

Silicates in orthopedics and bone tissue engineering materials

Zhou

Zhang

Mankoci

et al. 2017

J Biomedical Materials Res

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Following the success of silicate-based glasses as bioactive materials, silicates are believed to play important roles in promoting bone formation and have therefore been considered to provide a hydroxyapatite (HAP) surface layer capable of binding to bone as well as potentially being a pro-osteoinductive factor. Natural silicate minerals and silicate-substituted HAPs are also being actively investigated as orthopaedic bone and dental biomaterials for application in tissue engineering. However, the mechanisms for the proposed roles of silicate in these materials have not been fully understood and are controversial. Here, we review the potential roles of silicate for bone tissue engineering applications and recent breakthroughs in identifying the cellular-level molecular mechanisms for the osteoinductivity of silica. The goal of this article is to inspire new ideas for the rational design of third-generation cell-and gene-affecting biomaterials. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2090-2102, 2017.

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Orthosilicic acid, Si(OH)4, stimulates osteoblast differentiation in vitro by upregulating miR-146a to antagonize NF-κB activation

Cited by 69 publications

References 69 publications

Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

Osteoblast-like cell responses to silicate ions released from 45S5-type bioactive glass and siloxane-doped vaterite

Silicates in orthopedics and bone tissue engineering materials

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