Orthopaedic-implant infections by Escherichia coli: Molecular and phenotypic analysis of the causative strains

Crémet, Lise; Corvec, Stéphane; Bémer, Pascale; Bret, Laurent; Lebrun, Cécile; Lesimple, B.; Miègeville, Anne-Françoise; Reynaud, Alain; Lepelletier, Didier; Caroff, Nathalie

doi:10.1016/j.jinf.2011.11.010

Cited by 73 publications

(58 citation statements)

References 33 publications

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“…Our results illustrated a higher frequency of fim, pap and sfa operons compared with other virulence markers, which may suggest a crucial role of the adhesinencoding operons in UPEC causing UTI. The distribution of the other studied virulent genes found among the studied isolates belonged to group B2 was also similar to other reported data (25,26). We found a smaller proportions among isolates belonged to phylogenetic group A.…”

Section: Discussionsupporting

confidence: 91%

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

Rahdar¹,

Rashki

Miri³

2014

Avicenna J Clin Microbiol Infect

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Background: Escherichia coli is one of the most causative pathogen of urinary tract infection. Urinary tract infections (UTIs) are the second most common cause of morbidity and remain a serious health concern among the clinicians. The severity of UTI caused by uropathogenic E. coli (UPEC) is due to the expression of a wide spectrum of virulent factors such as adhesin coding operons. Little is known about the relationship between the E. coli genetic background and the acquisition of adhesin coding operons in UPEC isolates. Objectives: The aim of this study was to determine the prevalence of adhesin coding operons in UPEC isolates belonged to phylogenetic group B2 and A collected from patients suffering from UTI. Materials and Methods: A total 100 UPEC isolates were used for DNA extraction by the boiling lysis. The analysis of phylogenetic groups, along with detection of adhesin coding operons was performed by Multiplex-PCR method. Associations were assessed between afa, fim, foc, pap and sfa operons among to 55 B2 and 17 A groups E. coli isolates. Statistical analysis was performed using Fisher exact test. Results: Phylogenetic analysis showed that 55 and 17 of 100 UPEC isolates belonged to the B2 and A phylogenetic groups, respectively. The afa, fim, foc, pap and sfa operons were present in five (9.09%), 55 (100%), 16 (29.09%), 46 (83.63%) and 46 (83.63%) of UPEC isolates belonged to phylogenetic group B2, and two (12.50%), 14 (87.50%), one (6.25%), two (12.5%) and 12 (75%) of isolates belonged to phylogenetic group A, respectively. Statistical analysis showed that pap gene was significantly more frequently detected in phylogenetic group B2 (P < 0.05). Conclusions:The UPEC isolates belonging to group B2 harbored a greater number of adhesin coding operons than strains from phylogenetic groups A.

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Section: Discussionsupporting

confidence: 91%

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

Rahdar¹,

Rashki

Miri³

2014

Avicenna J Clin Microbiol Infect

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“…Little has previously been reported on possible differences in biofilm production between serotypes or serogroups of E. coli (30), although differences between strains have been published by several (31)(32)(33)(34). When comparing serotypes in our study, we aimed at reducing bias by having relatively large groups of isolates with as low an epidemiological relationship as possible.…”

Section: Discussionmentioning

confidence: 99%

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Nesse

Sekse

Berg

et al. 2014

Appl Environ Microbiol

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The biofilm-producing abilities of potentially human-pathogenic serotypes of Escherichia coli from the ovine reservoir were studied at different temperatures and on different surfaces. A possible influence of the hydrophobicity of the bacterial cells, as well as the presence of two virulence factors, the Shiga toxin-encoding (Stx) bacteriophage and the eae gene, was also studied. A total of 99 E. coli isolates of serotypes O26:H11, O103:H2, and O103:H25 isolated from sheep feces were included. The results show that isolates of all three E. coli serotypes investigated can produce biofilm on stainless steel, glass, and polystyrene at 12, 20, and 37°C. There was a good general correlation between the results obtained on the different surfaces. E. coli O103:H2 isolates produced much more biofilm than those of the other two serotypes at all three temperatures. In addition, isolates of serotype O26:H11 produced more biofilm than those of O103:H25 at 37°C. The hydrophobicity of the isolates varied between serotypes and was also influenced by temperature. The results strongly indicated that hydrophobicity influenced the attachment of the bacteria rather than their ability to form biofilm once attached. Isolates with the eae gene produced less biofilm at 37°C than isolates without this gene. The presence of a Stx bacteriophage did not influence biofilm production. In conclusion, our results show that potentially human-pathogenic E. coli from the ovine reservoir can form biofilm on various surfaces and at several temperatures relevant for food production and handling.

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“…Escherichia coli is one of the predominant organisms among Gram-negative bacilli causing PJI (6,7,11,12). In this organism, the emergence of extended-spectrum-␤-lactamase (ESBL)-producing strains harboring the plasmid-acquired gene for CTX-M15 ␤-lactamase is of particular concern (13,14).…”

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confidence: 99%

Activities of Fosfomycin, Tigecycline, Colistin, and Gentamicin against Extended-Spectrum-β-Lactamase-Producing Escherichia coli in a Foreign-Body Infection Model

Corvec

Tafin

Bétrisey

et al. 2013

Antimicrob Agents Chemother

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104

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Limited antimicrobial agents are available for the treatment of implant-associated infections caused by fluoroquinolone-resistant Gram-negative bacilli. We compared the activities of fosfomycin, tigecycline, colistin, and gentamicin (alone and in combination) against a CTX-M15-producing strain of Escherichia coli (Bj HDE-1) in vitro and in a foreign-body infection model. The MIC and the minimal bactericidal concentration in logarithmic phase (MBC log ) and stationary phase (MBC stat ) were 0.12, 0.12, and 8 g/ml for fosfomycin, 0.25, 32, and 32 g/ml for tigecycline, 0.25, 0.5, and 2 g/ml for colistin, and 2, 8, and 16 g/ml for gentamicin, respectively. In time-kill studies, colistin showed concentration-dependent activity, but regrowth occurred after 24 h. Fosfomycin demonstrated rapid bactericidal activity at the MIC, and no regrowth occurred. Synergistic activity between fosfomycin and colistin in vitro was observed, with no detectable bacterial counts after 6 h. In animal studies, fosfomycin reduced planktonic counts by 4 log 10 CFU/ml, whereas in combination with colistin, tigecycline, or gentamicin, it reduced counts by >6 log 10 CFU/ml. Fosfomycin was the only single agent which was able to eradicate E. coli biofilms (cure rate, 17% of implanted, infected cages). In combination, colistin plus tigecycline (50%) and fosfomycin plus gentamicin (42%) cured significantly more infected cages than colistin plus gentamicin (33%) or fosfomycin plus tigecycline (25%) (P < 0.05). The combination of fosfomycin plus colistin showed the highest cure rate (67%), which was significantly better than that of fosfomycin alone (P < 0.05). In conclusion, the combination of fosfomycin plus colistin is a promising treatment option for implant-associated infections caused by fluoroquinolone-resistant Gram-negative bacilli.

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Orthopaedic-implant infections by Escherichia coli: Molecular and phenotypic analysis of the causative strains

Cited by 73 publications

References 33 publications

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

Comparison of the Common Adhesin Coding Operons Distribution in Uropathogenic and Phylogenetic Group B2 and A Escherichia coli Isolates

Potentially Pathogenic Escherichia coli Can Form a Biofilm under Conditions Relevant to the Food Production Chain

Activities of Fosfomycin, Tigecycline, Colistin, and Gentamicin against Extended-Spectrum-β-Lactamase-Producing Escherichia coli in a Foreign-Body Infection Model

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