Orthomyxoviruses: Influenza

Cox, Nancy J.; Donis, Ruben O.; Kawaoka, Yoshihiro

doi:10.1002/9780470688618.taw0238

Cited by 13 publications

(21 citation statements)

References 783 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PDAs are associated with a constellation of genetic alterations in oncogenes and tumor suppressor genes, the four most frequent being observed in the K-RAS, TP53, SSMAD4/DPC4, and CDKN2A/p16 genes (3,9,70). The status of these four genes has been described previously for all six pancreatic cell lines included in this study (3,70); however, no immediate associations can be made by comparing the individual cell genotypes with their phenotypes with regard to sensitivity to influenza virus-induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…On rare occasions, the HA from an avian IAV has successfully overcome this barrier and adapted to bind ␣-2,6 linkages, causing the pandemics of 1918, 1957, and 1968. Such viruses then become established in the human population and cause seasonal influenza epidemics (9). The H1N1 2009 pandemic was instead caused by a triple reassortant carrying genes from avian and swine influenza viruses; however, in this case, the swine HA was already specific for the human receptor (19).…”

mentioning

confidence: 99%

“…Influenza A viruses (IAVs) of the family Orthomyxoviridae are naturally circulating viruses of aquatic birds, from which a total of 16 diverse hemagglutinin (HA) and 9 neuraminidase (NA) types have been isolated in various combinations (8,9). Additional subtypes have recently been discovered in bats but represent highly genetic lineages (10).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Oncolytic Activity of Avian Influenza Virus in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Kasloff

Pizzuto

Silic-Benussi

et al. 2014

J Virol

View full text Add to dashboard Cite

Pancreatic ductal adenocarcinoma (PDA) is the most lethal form of human cancer, with dismal survival rates due to late-stage diagnoses and a lack of efficacious therapies. Building on the observation that avian influenza A viruses (IAVs) have a tropism for the pancreas in vivo, the present study was aimed at testing the efficacy of IAVs as oncolytic agents for killing human PDA cell lines. Receptor characterization confirmed that human PDA cell lines express the alpha-2,3-and the alpha-2,6-linked glycan receptor for avian and human IAVs, respectively. PDA cell lines were sensitive to infection by human and avian IAV isolates, which is consistent with this finding. Growth kinetic experiments showed preferential virus replication in PDA cells over that in a nontransformed pancreatic ductal cell line. Finally, at early time points posttreatment, infection with IAVs caused higher levels of apoptosis in PDA cells than gemcitabine and cisplatin, which are the cornerstone of current therapies for PDA. In the BxPC-3 PDA cell line, apoptosis resulted from the engagement of the intrinsic mitochondrial pathway. Importantly, IAVs did not induce apoptosis in nontransformed pancreatic ductal HPDE6 cells. Using a model based on the growth of a PDA cell line as a xenograft in SCID mice, we also show that a slightly pathogenic avian IAV significantly inhibited tumor growth following intratumoral injection. Taken together, these results are the first to suggest that IAVs may hold promise as future agents of oncolytic virotherapy against pancreatic ductal adenocarcinomas. IMPORTANCEDespite intensive studies aimed at designing new therapeutic approaches, PDA still retains the most dismal prognosis among human cancers. In the present study, we provide the first evidence indicating that avian IAVs of low pathogenicity display a tropism for human PDA cells, resulting in viral RNA replication and a potent induction of apoptosis in vitro and antitumor effects in vivo. These results suggest that slightly pathogenic IAVs may prove to be effective for oncolytic virotherapy of PDA and provide grounds for further studies to develop specific and targeted viruses, with the aim of testing their efficacy in clinical contexts.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Oncolytic Activity of Avian Influenza Virus in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Kasloff

Pizzuto

Silic-Benussi

et al. 2014

J Virol

View full text Add to dashboard Cite

show abstract

“…Influenza viruses belong to the family Orthomyxoviridae , consisting of five genera that include influenza A virus, influenza B virus, influenza C virus, Thogotovirus and Isavirus, as defined by the International Committee on Taxonomy of Viruses [1]. On the basis of their nucleoprotein (NP) and matrix (M) protein antigens, influenza A and B viruses contain hemagglutinin (HA) and neuraminidase (NA) activities, whereas influenza C virus has no NA, but does have a hemagglutinin–esterase fusion (HEF) protein [1].…”

Section: Introductionmentioning

confidence: 99%

Advancements in the development of subunit influenza vaccines

Zhang

Zheng

et al. 2015

Microbes and Infection

View full text Add to dashboard Cite

The ongoing threat of influenza epidemics and pandemics has emphasized the importance of developing safe and effective vaccines against infections from divergent influenza viruses. In this review, we first introduce the structure and life cycle of influenza A viruses, describing major influenza A virus-caused pandemics. We then compare different types of influenza vaccines and discuss current advancements in the development of subunit influenza vaccines, particularly those based on nucleoprotein (NP), extracellular domain of matrix protein 2 (M2e) and hemagglutinin (HA) proteins. We also illustrate potential strategies for improving the efficacy of subunit influenza vaccines.

show abstract

“…The virus is classified according to the hemagglutinin and neuraminidase subtypes, which define its antigenicity, present in its envelope. The epidemiological success of influenza A is mainly attributed to its ability to carry out genetic reassortment, which is responsible for the generation of new strains of viruses with pandemic potential [1,2].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of two rapid influenza tests in comparison to reverse transcription-PCR for influenza A diagnosis

Zazueta-García

Canizalez-Roman

Flores-Villaseñor

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: The influenza A virus is responsible for high morbidity and mortality in children and adults worldwide. Thus, a rapid, sensitive, and specific diagnosis tool is required. Methodology: An immunofluorescence assay (DFA) and a lateral-flow immunochromatographic assay were compared with RT-PCR for detection of the influenza A virus in 113 nasopharyngeal wash samples obtained from pediatric patients. Samples were collected between July and December 2009, during the pandemic outbreak of influenza A H1N1/09. Results: The sensitivity, specificity, and positive and negative predictive values obtained for the DFA were 68.97%, 76.63%, 75.47%, and 70%, respectively, while the values obtained for the immunochromatographic assay were 58.62%, 81.82%, 77.27%, and 65.22%, respectively. The frequency of the influenza A virus was 51.33%, and a total of 27 samples were positive for the pandemic influenza A H1N1/09. Conclusions: DFA and the immunochromatographic assay can be important tools for patient care during influenza season and in outbreaks as they usually provide results within 45 minutes. Furthermore, positive results in conjunction with the patient's symptoms could provide a correct diagnosis, thus facilitating appropriate patient management. Nonetheless, the results of these assays still require confirmation by RT-PCR.

show abstract

Orthomyxoviruses: Influenza

Cited by 13 publications

References 783 publications

Oncolytic Activity of Avian Influenza Virus in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Oncolytic Activity of Avian Influenza Virus in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Advancements in the development of subunit influenza vaccines

Effectiveness of two rapid influenza tests in comparison to reverse transcription-PCR for influenza A diagnosis

Contact Info

Product

Resources

About