“…In their pioneer work, DNA strands with unique secondary structures can catalyze the transesterification of RNA phosphate diester bonds. Since then, a plethora of artificial DNA sequences as active enzymes were selected by Systematic Evolution of Ligands by Exponential enrichment (SELEX), which has become an important technology for Dzs library screening due to its excellent target analysis range and strong affinity ( Silverman, 2016 ; Kumar et al., 2019 ; Wang et al., 2021a ). Regioselective Dzs consisting of synthetic DNA single stands and different cofactors have been developed with diverse catalytic abilities ( Kumar et al., 2019 ; Hollenstein, 2015 ), e.g., DNA/RNA cleavage or ligation ( Zhou et al., 2015 , 2016 ; Praetorius et al., 2017 ; Wu et al., 2017 ; Huang et al., 2020 ; Purtha et al., 2005 ), phosphorylation ( Camden et al., 2016 ), hydrolysis ( Chandra et al., 2009 ), and alkyne-azide “click’ cycloaddition ( Liu et al., 2020a ).…”