DNA-based enzymatic systems and their applications

Jiao, Yunfei; Shang, Yingxu; Li, Na; Ding, Baoquan

doi:10.1016/j.isci.2022.104018

Cited by 16 publications

(16 citation statements)

References 103 publications

(156 reference statements)

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“… 45 For other non-nucleic acid targets, such as protein molecules, the recognizing aptamers, 46 the binding-specific nucleic acid sequences, 47 or responsive peptide-DNA fragments 48 could incorporate into the responsive modules. In addition, targets such as metal ions, pH, and other small molecules in the environment usually activate the responsive modules by responsive nucleic acid structures such as metal ion-responsive DNAzyme, 49 pH-sensitive i-motif, 50 or other specific nucleic acid structures.…”

Section: Building Blocks Of Au-dna Nanomachinesmentioning

confidence: 99%

Advances in self-assembled Au-DNA nanomachines

Zhang¹,

Xu²,

Zheng³

et al. 2023

iScience

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Section: Building Blocks Of Au-dna Nanomachinesmentioning

confidence: 99%

Advances in self-assembled Au-DNA nanomachines

Zhang¹,

Xu²,

Zheng³

et al. 2023

iScience

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“…Besides the static DNA origami structures, the dynamic DNA nanotechnology enables the construction of various reconfigurable DNA origami structures induced by the hybridization of short DNA, aptamer–ligand interactions, temperature, pH, ion and electric field with controlled translational, rotational or more sophisticated movement [ 56 , 57 , 58 , 59 , 60 , 61 , 62 ]. These dynamic DNA structures have been widely applied in the fields of drug delivery, diagnosis, biosensing and biocatalysis, which has been reviewed previously [ 63 , 64 , 65 , 66 , 67 ].…”

Section: Catalytic Enhancement Of Single Type Of Enzyme Assembled On ...mentioning

confidence: 99%

Mechanistic Aspects for the Modulation of Enzyme Reactions on the DNA Scaffold

et al. 2022

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Cells have developed intelligent systems to implement the complex and efficient enzyme cascade reactions via the strategies of organelles, bacterial microcompartments and enzyme complexes. The scaffolds such as the membrane or protein in the cell are believed to assist the co-localization of enzymes and enhance the enzymatic reactions. Inspired by nature, enzymes have been located on a wide variety of carriers, among which DNA scaffolds attract great interest for their programmability and addressability. Integrating these properties with the versatile DNA–protein conjugation methods enables the spatial arrangement of enzymes on the DNA scaffold with precise control over the interenzyme distance and enzyme stoichiometry. In this review, we survey the reactions of a single type of enzyme on the DNA scaffold and discuss the proposed mechanisms for the catalytic enhancement of DNA-scaffolded enzymes. We also review the current progress of enzyme cascade reactions on the DNA scaffold and discuss the factors enhancing the enzyme cascade reaction efficiency. This review highlights the mechanistic aspects for the modulation of enzymatic reactions on the DNA scaffold.

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“…[ 10 ] As gene‐regulatory agents, RNA‐cleaving DNAzymes can recognize and silence specific gene sequences. [ 11 ] In addition, DNAzymes possess tremendous merits, such as high selectivity, [ 12 ] multiple turnover rates, no permanent influence on the genome, simple design, and suitability for large‐scale rapid production, which have great clinical application value. [ 13 ] Through considerable exploration over the past two decades, DNAzyme‐related therapeutics have covered cancer, viral, asthma, cardiovascular, inflammatory, and central nervous system diseases.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic DNAzymes: From Structure Design to Clinical Applications

et al. 2023

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Therapeutic DNAzymes have unceasingly intrigued the scientific community owing to their prosperous gene regulation capability. The efficacy of DNAzymes against many types of diseases has been extensively studied for over two decades. However, the high expectations for DNAzymes are still not translated to the clinic because of their low effectiveness in vivo. Over the last five years, several aspects have been considered to optimize DNAzyme‐integrated therapeutics, including structural stability, mechanism exploration, cell internalization rate, cofactor activation, and off‐target effects. Hence, this review first discusses the early monotherapy and structural design of DNAzymes. Subsequently, the latest modes of action are reviewed, followed by an elaboration on structural stabilization strategies considering the catalytic core and substrate‐binding arms. DNAzyme‐based synergistic therapy is then examined, highlighting responsive carrier construction, synergistic effects, the latest discovered advanced functions, and off‐target concerns. Beyond this, key clinical advances in DNAzyme‐based therapy are elucidated by showcasing clinical progress. Finally, future trends and development challenges for DNAzyme‐powered therapeutics in the coming years are discussed in detail.

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DNA-based enzymatic systems and their applications

Cited by 16 publications

References 103 publications

Advances in self-assembled Au-DNA nanomachines

Advances in self-assembled Au-DNA nanomachines

Mechanistic Aspects for the Modulation of Enzyme Reactions on the DNA Scaffold

Therapeutic DNAzymes: From Structure Design to Clinical Applications

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