Orosomucoid, seromucoid and haptoglobin in serum during adjuvant arthritis of the rat

Sternberg, M.; Peyroux, J.; Engler, R; Grochulski, A; Lesaget, C.; Lagrue, G; Jayle, M F

doi:10.1007/bf01927730

Cited by 9 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the secondary inflammatory phase of adjuvant arthritis, the degree of swelling was much greater than the primary phase but the change in levels of APPs were less than would be predicted had levels of APPs been directly correlated to the degree of inflammation. The changes in levels of haptoglobin, seromucoid and caeruloplasmin were similar to results obtained by other researchers [17,26,27]. This lack of change, or down-regulation of response, during the secondary phase could be caused by a variety of factors: release of cytokine inhibitors, reduced release of cytokines or the liver synthesis of the proteins reaching a maximal level.…”

Section: Discussionsupporting

confidence: 74%

“…Previous studies had shown that APPs in plasma stored at ¹20 ЊC were stable for up to one year. Analytical methods for rat APPs have been described in detail [14] but, in brief, seromucoid was measured by perchloric and phosphotungstic acid precipitation [17], haptoglobin by its peroxidase activity [18], caeruloplasmin by its oxidative action on p-phenylenediamine to produce a coloured product [19] and albumin levels were measured using the bromocresol green method [20]. Data from the Cobas-bio were electronically collected using an Epson HX-20 computer and transferred to the IBM PC-XT computer.…”

Section: Acute Phase Protein Measurementmentioning

confidence: 99%

See 1 more Smart Citation

A simple inflammation model that distinguishes between the actions of anti-inflammatory and anti-rheumatic drugs

Lewis

Bishop

Aspinall

1998

Inflammation Research

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Acute Phase Protein Measurementmentioning

confidence: 99%

A simple inflammation model that distinguishes between the actions of anti-inflammatory and anti-rheumatic drugs

Lewis

Bishop

Aspinall

1998

Inflammation Research

View full text Add to dashboard Cite

show abstract

“…These are the subcutaneous injection of turpentine (Liao et al 1986;Bu¨rger et al 1987;Myers and Fleck 1988;Jinbo et al 2002) and the injection of FCA into the footpad (Sternberg et al 1974;Bu¨rger et al 1987;Connolly et al 1988;Tanahashi et al 1998). However, a third, less frequently used model involving the intraperitoneal (i.p.)…”

Section: Introductionmentioning

confidence: 97%

“…Hp has also been investigated as an indicator of inflammation in the rat. Sternberg et al (1974) studied the role of Hp in adjuvant arthritis in the rat, and Gilbertsen (1986), using a collagen-arthritis model, measured Hp levels to assess the effectiveness of antiarthritic drug therapy.…”

Section: Introductionmentioning

confidence: 99%

Markers of experimental acute inflammation in the Wistar Han rat with particular reference to haptoglobin and C-reactive protein

Giffen

Turton

Andrews

et al. 2003

Archives of Toxicology

View full text Add to dashboard Cite

C-reactive protein (CRP), haptoglobin (Hp) and fibrinogen (Fbgn) are acute phase reactants (APRs), the blood levels of which increase during acute inflammation. However, although the levels of these APRs are used to monitor inflammation in man, their usefulness and sensitivity as markers of inflammation in rodents are less clear. We therefore wished to evaluate, in a comparative fashion, a prototype immunoassay for serum CRP, a commercial assay for serum Hp, and an automated assay for Fbgn, using a model of acute inflammation in the rat. Additionally, pro-inflammatory cytokines and serum protein fractions were also measured. The model of inflammation used was the intraperitoneal injection of Freund's complete adjuvant (FCA). In a concluding experiment, findings with Hp in the FCA rat model were validated in a toxicologically relevant study involving the induction of acute hepatic inflammation using the model hepatotoxicant carbon tetrachloride (CCl(4)). Female Wistar Han rats were treated with a single injection of FCA in a dose-response study (1.25-10.0 ml/kg, sampling at 36 h) and two time-course studies (over 40 h and 21 days). In a final experiment, rats were dosed with CCl(4) at 0.8 ml/kg and sampled over a 17-day period. In FCA and CCl(4) experiments, serum/plasma was prepared and tissues taken at autopsy for histological assessment (CCl(4) study only). In the dose-response study, serum CRP, Hp and plasma Fbgn were increased at all FCA dose levels at 36 h post-dosing. Serum alpha(2) and beta(1) globulin fractions were also increased, while albumin levels were decreased. In the 40-h time-course study, CRP levels peaked at 25-40 h post-dosing, to approximately 120% of control (as 100%). Hp levels increased to a maximum at 25 and 40 h post-dosing with values greater than 400% of control, and alpha(2) and beta(1) globulin fractions peaked at 30 and 40 h post-dosing to 221 and 187% of control, respectively. Increased serum interleukin-6 (IL-6) and interleukin-1beta (IL-1beta) levels peaked at 20 h (11-fold) and 25 h (19-fold), respectively. In a 21-day time-course study, no increased CRP levels were measured despite elevated levels of Hp, which peaked at 36 h (approximately 7-fold above control), and remained elevated up to 21 days. IL-6 and IL-1beta levels peaked at 12 h (19-fold) and 24 h (28-fold), respectively. Liver histopathology of animals treated with CCl(4) showed centrilobular hepatocellular degeneration and necrosis (most significant at 36 h) with an inflammatory response (most significant at 48 h). Resolution of the lesion was complete by 4 days post-dosing. Serum alanine aminotransferase, aspartate aminotransferase and glutamate dehydrogenase levels peaked at 36 h post-dosing. Hp levels increased maximally at 48 h (426% of control). We conclude that serum CRP is a poor marker of acute inflammation in the rat in comparison with serum Hp and plasma Fbgn. Between Hp and Fbgn, serum Hp is shown to be the most sensitive and useful marker of acute inflammation.

show abstract

“…Orosomucoid was determined by radial immunodiffusion [21]. Monospecificity of the antiorosomucoid antiserum was assessed by a unique precipitation line on immunoelectrophoresis against total rat scrum.…”

Section: Methodsmentioning

confidence: 99%

Orosomucoid and Haptoglobin Excretion in Rat Glomerulonephritis and Tubulonephritis

Pironneau¹,

Sternberg²,

Moisy³

et al. 1974

Nephron

View full text Add to dashboard Cite

Haptoglobin and orosomucoid were detected and measured in concentrated normal rat urine by radial immunodiffusion. Normal haptoglobin clearance was 33 nl/min (190 nl/ min/kg) and normal orosomucoid clearance 146 nl/min (840 nl/ min/kg). During nephrotoxic serum glomerulonephritis both glycoproteins increased considerably in urine in two phases; in serum, after an early peak, haptoglobin returned to the normal level and orosomucoid fell below the normal value. During mercuric chloride tubulonephritis, an early sharp peak of both glycoproteins was observed chiefly in urine but also in serum.

show abstract

Orosomucoid, seromucoid and haptoglobin in serum during adjuvant arthritis of the rat

Cited by 9 publications

References 9 publications

A simple inflammation model that distinguishes between the actions of anti-inflammatory and anti-rheumatic drugs

A simple inflammation model that distinguishes between the actions of anti-inflammatory and anti-rheumatic drugs

Markers of experimental acute inflammation in the Wistar Han rat with particular reference to haptoglobin and C-reactive protein

Orosomucoid and Haptoglobin Excretion in Rat Glomerulonephritis and Tubulonephritis

Contact Info

Product

Resources

About