Oropouche virus is detected in peripheral blood leukocytes from patients

Luna, Luciano Kleber de Souza; Rodrigues, Alcir Humberto; Santos, Rodrigo I.; Sesti-Costa, Renata; Criado, Miriã Ferreira; Martins, Ronaldo Teixeira; Silva, Maria Lúcia; Delcaro, Luana; Proença-Módena, José Luiz; Figueiredo, Luíz Tadeu Moraes; Acrani, Gustavo Olszanski; Arruda, Eurico

doi:10.1002/jmv.24722

Cited by 20 publications

(24 citation statements)

References 15 publications

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“…While these were residual samples that may have been degraded by freeze/thaw cycles, our findings are consistent with prior results; JCV has never been isolated from human samples, and one study demonstrated no detectable viremia in experimentally infected rhesus macaques, despite seroconversion [34]. In general for orthobunyaviruses, it has been challenging to grow virus [35] or detect viral RNA in human samples; the few exceptions to this have included the detection of RNA from LACV in brain tissue [36], Oropouche virus in leukocytes [37], and Cache Valley virus in the CSF of an immunocompromised patient [38]. Overall, our results suggest that viral RNA detection techniques are unlikely to be useful for routine JCV diagnostics, likely because of lowlevel or short viremia.…”

Section: Discussionsupporting

confidence: 89%

Jamestown Canyon virus in Massachusetts: clinical case series and vector screening

Kinsella

Paras

Smole

et al. 2020

Emerging Microbes & Infections

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Jamestown Canyon virus (JCV) is a neuroinvasive arbovirus that is found throughout North America and increasingly recognized as a public health concern. From 2004 to 2012, an average of 1.7 confirmed cases were reported annually in the United States, whereas from 2013 to 2018 this figure increased over seventeen-fold to 29.2 cases per year. The rising number of reported human infections highlights the need for better understanding of the clinical manifestations and epidemiology of JCV. Here, we describe nine patients diagnosed with neuroinvasive JCV infection in Massachusetts from 2013, the year of the first reported case in the state, to 2017. Because current diagnostic testing relies on serology, which is complicated by cross-reactivity with related orthobunyaviruses and can be negative in immunosuppressed patients, we developed and evaluated an RT-qPCR assay for detection of JCV RNA. We tested this on the available archived serum from two patients, but did not detect viral RNA. JCV is transmitted by multiple mosquito species and its primary vector in Massachusetts is unknown, so we additionally applied the RT-qPCR assay and confirmatory RNA sequencing to assess JCV prevalence in a vector candidate, Ochlerotatus canadensis. We identified JCV in 0.6% of mosquito pools, a similar prevalence to neighboring Connecticut. We assembled the first Massachusetts JCV genome directly from a mosquito sample, finding high identity to JCV isolates collected over a 60year period. Further studies are needed to reconcile the low vector prevalence and low rate of viral evolutionary change with the increasing number of reported cases.

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Section: Discussionsupporting

confidence: 89%

Jamestown Canyon virus in Massachusetts: clinical case series and vector screening

Kinsella

Paras

Smole

et al. 2020

Emerging Microbes & Infections

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“…OROV is also distributed to areas where hematophagous mosquito species Culex quinquefasciatus , Coquillettidia venezuelensis , and Aedes (Ochlerotatus) serratus can breed and may be naturally infected by the virus [ 1 , 32 , 33 ]. It is well documented that, in 1960, OROV was isolated from mosquito species Cq.…”

Section: Arthropod Vectorsmentioning

confidence: 99%

“…serratus , Cx. quinquefasciatus and midges of the genus Culicoides have been reported to be likely vectors in the sylvatic cycle as indicated by epidemiological and laboratory based evidence [ 4 , 33 , 41 , 43 , 44 ].…”

Section: Transmission Cyclesmentioning

confidence: 99%

“…Mato Grosso is surrounded by states where OROV is endemic, has a high rate of population growth due to timber, ranching and agricultural development, and is acclaimed for its intriguing biodiversity and the world’s largest wetlands; in fact, the state has all the distinctive features favoring vectors’ preservation and OROV maintenance [ 57 ]. In 2016, the first OROV fever case outside the Amazon region was reported [ 33 ]. The OROV antigen was detected in peripheral blood leukocytes by indirect immunofluorescence from two febrile patients in São Paolo, raising concerns on the occurrence of OROV fever out of the Amazon region [ 33 ].…”

Section: Disease Epidemiologymentioning

confidence: 99%

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Oropouche Fever: A Review

Sakkas

Bozidis

Franks

et al. 2018

Viruses

100

117

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Oropouche fever is an emerging zoonotic disease caused by Oropouche virus (OROV), an arthropod transmitted Orthobunyavirus circulating in South and Central America. During the last 60 years, more than 30 epidemics and over half a million clinical cases attributed to OROV infection have been reported in Brazil, Peru, Panama, Trinidad and Tobago. OROV fever is considered the second most frequent arboviral febrile disease in Brazil after dengue fever. OROV is transmitted through both urban and sylvatic transmission cycles, with the primary vector in the urban cycle being the anthropophilic biting midge Culicoides paraensis. Currently, there is no evidence of direct human-to-human OROV transmission. OROV fever is usually either undiagnosed due to its mild, self-limited manifestations or misdiagnosed because its clinical characteristics are similar to dengue, chikungunya, Zika and yellow fever, including malaria as well. At present, there is no specific antiviral treatment, and in the absence of a vaccine for effective prophylaxis of human populations in endemic areas, the disease prevention relies solely on vector control strategies and personal protection measures. OROV fever is considered to have the potential to spread across the American continent and under favorable climatic conditions may expand its geographic distribution to other continents. In view of OROV’s emergence, increased interest for formerly neglected tropical diseases and within the One Health concept, the existing knowledge and gaps of knowledge on OROV fever are reviewed.

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“…Fenômenos hemorrágicos (petequias, epistaxe e sangramento gengival) na doença por OROV foram relatados em um surto ocorrido em Manaus (MOURÃÃO et al, 2009). O vírus encontra-se presente e se dissemina em leucócitos do sangue periférico (DE SOUZA LUNA et al, 2017). Embora a maioria dos pacientes se recupere dentro de 2 a 3 semanas sem seqüelas de longo prazo (PINHEIRO et al, 1981a), recidivas são comuns (PINHEIRO et al, 1981a).…”

Section: Eia-iccunclassified

Desenvolvimento de um modelo murino para estudo da resposta imune conferida pela proteína do Nucleocapsídeo do vírus Oropouche

Zapana¹

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"Dedicado a meus queridos pais Marcelina e Fortunato, e aos meus irmãos Braulio, Armando, Enrique, Guido, Harold, Belisario e Carolina" AGRADECIMENTOSA Deus por ter me concedido a oportunidade de fazer meu mestrado neste maravilhoso pais, Brasil.Ao Prof. Luiz Tadeu Moraes Figueiredo, por ter sido meu orientador durante este trabalho e pelo respeito e dedicação a mim neste tempo.Agradeço ao Prof. Eurico Arruda e Jose Luiz Proença, foram essenciais para minha formação.Agradeço aos seguintes amigos pesquisadores que colaboraram diretamente com o trabalho, através de auxilio em muitos dos experimentos envolvidos. Sem ajuda dessas pessoas este trabalho não teria sido possível: Alberto Anastacio Amarilla, Marcilio Jorge, Marcus Silva, Marjorie Cornejo, Anibal Oliveira.Aos professores e alunos do Centro de Pesquisa em Virologia, pela constante troca de conhecimento e pelo convívio.Agradeço à funcionaria Soraya Jabur Badra pelo suporte emocional que sempre me deu e a Maria Lucia pelo apoio nos experimentos e pelo carinho.A meus colegas de Laboratório: Gilberto, Danilo, William, Leonardo, Lucia, Aline, Leonardo, Felipe, Marilia, Angelica.A meus prezados amigos: Jenny Romero, Tatiana Veloso, Tiago Alves, Poliane Crisostomo, Juan Chuctaya, Felipe Canto, Carlismari, por serem meus irmãos neste tempo.A meus amigos: Nayara Ruiz, Daysi Alves, Hudson Alves, por ter me acolhido sempre na sua casa como uma integrante dela e pelo carinho.A todos os amigos e familiares pelo carinho e amor.Muito obrigada."A vida é nos concedida apenas como empréstimo; e a indagação de cada um devia ser: como poderei investir os meus talentos com o melhor proveito" como poderei fazer o máximo para a Gloria de Deus e o benefício de meus semelhantes? Pois a vida só tem valor se usada para a conquista desses objetivos" Ellen G. White Priscila R. Mamani Z.Desenvolvimento de um modelo murino para estudo da resposta imune conferida pela proteína do Nucleocapsídeo do vírus Oropouche. PINHEIRO et al., 1982). Ocasionalmente, pode ocorrer exantema e infecção do sistema nervoso central. Não há relato de casos fatais por OROV (LEDUC; PINHEIRO F, 1989). A maioria dos sintomas desaparecem entre 3 a 5 dias, embora a mialgia possa durar 1 ou 2 semanas. Até 10 dias após o fim do quadro febril, cerca de 50% dos pacientes podem apresentar recorrência de alguns sintomas (VASCONCELOS et al., 1989). No entanto, a viremia é detectável, na maior parte dos pacientes, 2 a 3 dias após o início da doença.Também, é possível que ocorra infecção por OROV em aerossol, como observado em acidente laboratorial (PINHEIRO et al., 1981 PINHEIRO et al., 1981a). Em um surto registrado em Santarém, Estado do Pará, a proporção de mulheres infectadas foi 2 vezes maior que na população masculina. Além disso, a febre do Oropouche afeta todos os grupos etários, embora em alguns surtos a incidência tenha sido maior em crianças e adultos jovens (PINHEIRO et al., 1976; PINHEIRO F et al., 2004). O ciclo viral e aspectos moleculares da patogêneseEstudos realizados sugerem que OROV é mantido na na...

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Oropouche virus is detected in peripheral blood leukocytes from patients

Cited by 20 publications

References 15 publications

Jamestown Canyon virus in Massachusetts: clinical case series and vector screening

Jamestown Canyon virus in Massachusetts: clinical case series and vector screening

Oropouche Fever: A Review

Desenvolvimento de um modelo murino para estudo da resposta imune conferida pela proteína do Nucleocapsídeo do vírus Oropouche

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