Ornithine decarboxylase activity and DNA synthesis in Morris hepatomas 51123-C and 7800

Pariza, Michael W.; Yanagi, Susumu; Gurr, James A.; Bushnell, Donald E.; Morris, Harold P.; Potter, Van R.

doi:10.1016/0024-3205(76)90271-x

Cited by 21 publications

(5 citation statements)

References 12 publications

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“…We have recently reported that poorly differentiated head and neck tumors with short patient survival had proportionately higher activities of the enzyme ornithine decarboxylase (ODC) compared to highly differentiated tumors (9). This finding suggests that polyamines may exert a growth regulatory action in clinical malignancy as described for experimental tumors (13,18,19,23). ODC is rate limiting for polyamine synthesis, and polyamines are associated with cell growth and division although their precise role in cell cycle regulation is not completely understood (19).…”

mentioning

confidence: 96%

Tumor cytokinetic effects of acute starvation versus polyamine depletion in tumor‐bearing mice

et al. 1991

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Previous investigations in our laboratory have demonstrated that both acute host starvation and polyamine depletion by means of the irreversible ODC-inhibitor (ODC = ornithine-decarboxylase) fluoro-methylornithine (DFMO) lead to pronounced growth retardation of rapidly proliferating tumors. The aim of this investigation was to elucidate how these different interventions affect cell kinetics and cell cycle phases in vivo. Adult nongrowing mice (C57Bl/J) bearing a poorly differentiated rapidly growing methylcholanthrene induced sarcoma were used. Combined measurements of bromodeoxyuridine incorporation into DNA and flow cytometric techniques were used. Starvation and DFMO treatment resulted in a prolonged cell cycle transit compared to freely fed animals. Tumor cells from DFMO-treated mice demonstrated an increased time for DNA synthesis and a relatively larger accumulation of cells in the G2M phase, whereas tumor cells from starved animals were accumulated in the G0G1 phase. The fractional cell loss of tumor cell during proliferation was calculated to be around 18% higher in DFMO-treated animals compared to starved and freely fed tumor-bearing mice. This study demonstrates that different mechanisms are involved in tumor growth suppression from substrate deficiency (starvation) and from inhibition of polyamine synthesis.

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confidence: 96%

Tumor cytokinetic effects of acute starvation versus polyamine depletion in tumor‐bearing mice

et al. 1991

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“…Although there are reports showing that ornithine decarboxylase activity is elevated in transplanted rat hepatomas (relative to the activity in the liver) and that the enzyme increases with hepatoma growth rate (Williams-Ashman et al, 1972), there is also evidence that these correlations do not occur consistently. Pariza et al (1976) point out that there are conflicting data from several laboratories in which the same hepatoma, e.g. hepatoma 7800, has been studied and that the combined evidence was not in conformity with the generalization that the activity of ornithine decarboxylase is proportional to tumour growth rate.…”

Section: Discussionmentioning

confidence: 86%

Ornithine decarboxylase and polyamines in liver and kidneys of rats on cyclical regimen of protein-free and protein-containing diets. Relationship to deoxyribonucleic acid synthesis in liver

Farwell

Miguez

Herbst

1977

Biochemical Journal

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1. The activity of ornithine decarboxylase in the liver and kidneys of rats maintained on a cyclical regimen of protein-free and protein-containing diets was investigated. There was a daily activation of the enzyme in response to the feeding of protein after 3 days feeding of protein-free diet. 2. The activation of ornithine decarboxylase in the liver and kidneys of rats re-fed on protein was demonstrable throughout 16 cycles of alternating 3-day periods of protein-free and protein-containing diets. The magnitude of the activation in the kidneys diminished from 20-fold stimulation in the first cycle to 5-fold stimulation (compared with animals fed with protein-free diet) in the later cycles of protein re-feeding. The activation of the enzyme in liver was decreased from 20-fold stimulation in the first cycle to approx. 10-fold stimulation in later cycles. 3. The concentration of spermidine was increased by approx. 50% in the liver of animals during cycling from protein-free to protein-containing diets. Spermine was unchanged, and putrescine was maintained at a low concentration approx. one-fifth to one-tenth that of spermidine after protein re-feeding. 4. The incorporation of [(3)H]thymidine into liver DNA was increased 10-fold in animals re-fed with protein compared with animals receiving protein-free diets. 5. The activation of ornithine decarboxylase by re-feeding of protein was inhibited 90% by the injection of propane-1,3-diamine during re-feeding. The stimulation of DNA synthesis was inhibited 60% by multiple injections of propane-1,3-diamine during the re-feeding of protein.

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“…Further experimentation will be neededto clarify these points and to determine the extent of relationship between cyclic nucleotides and AIB transport in cultured adult rat liver cells. Moreover, experiments comparing cultured cells from normal liver with cultured hepatoma cells may lead not only to a clearer understanding of hormonal regulation in liver (17), but also to the issue of whether there exists a systematic correlation between hepatic carcinogenesis and alterations in response to catecholamines (18)(19)(20)(21)25).…”

Section: Induction Of Camp and Aib Transport By Epimentioning

confidence: 99%

3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

Pariza¹,

Butcher²,

Becker³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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Ornithine decarboxylase activity and DNA synthesis in Morris hepatomas 51123-C and 7800

Cited by 21 publications

References 12 publications

Tumor cytokinetic effects of acute starvation versus polyamine depletion in tumor‐bearing mice

Tumor cytokinetic effects of acute starvation versus polyamine depletion in tumor‐bearing mice

Ornithine decarboxylase and polyamines in liver and kidneys of rats on cyclical regimen of protein-free and protein-containing diets. Relationship to deoxyribonucleic acid synthesis in liver

3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

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