3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

Pariza, Michael W.; Butcher, Fred R.; Becker, Joyce E.; Potter, Van R.

doi:10.1073/pnas.74.1.234

Cited by 39 publications

(11 citation statements)

References 21 publications

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“…Furthermore, propranolol blocked the epinephrine-induced increase in cellular cAMP, but not the increase in AIB uptake. On the basis of these and other results it was concluded that the epinephrine may stimulate transport by a cAMP-independent mechanism (Pariza et al, 1977). On the other hand, using hepatocyte suspensions, isoproterenol (100 p~x) was shown to stimulate AIB uptake (LeCam & Freychet, 1978 b).…”

Section: Catecholaminesmentioning

confidence: 95%

“…The catecholamines stimulate AIB uptake by isolated rat hepatocytes either in culture (Pariza, Butcher, Becker & Potter, 1977) or in freshly isolated cell suspensions (LeCam & Freychet, 1977b). The simulation of transport by epinephrine occurs predominantly via alpha-adrenergic receptors as shown by blockage of its effects by the alpha-blockers phentolamine and phenoxybenzamine, but not by the beta-antagonist propranolol (LeCam & Freychet, 1978b)o Epinephrine was almost 5 times more potent than norepinephrine, and caused an increase in Vmax without a change in K m. The stimulation requires de novo protein synthesis and becomes apparent only after a lag period of 1 to 2 hr (Pariza et al, 1977 ;LeCam & Freychet, 1978b).…”

Section: Catecholaminesmentioning

confidence: 99%

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Amino acid transport in isolated rat hepatocytes

Kilberg

1982

J. Membrain Biol.

217

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Improvements in the collagenase perfusion techniques have made isolated rat hepatocytes a popular model in which to study hepatic function. Our knowledge of hepatic amino acid transport has been advanced as a result of this methodology. Translocation across the hepatocyte plasma membrane can, in some instances, represent the rate-limiting step in the overall metabolism of certain amino acids. Furthermore, regulation of amino acid uptake by hepatocytes appears to play a role in diabetes, and perhaps in malignant transformation. Comparisons between normal adult hepatocytes and several hepatoma cell lines show basic differences in amino acid transport. There are at least eight distinct systems in normal hepatocytes for transport of the hormones. Systems A and N exhibit enhanced uptake rates after the cells have been maintained in the absence of extracellular amino acids, a phenomenon termed adaptive control. Further studies using isolated hepatocytes will increase our basic understanding of membrane transport processes and their regulation.

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Section: Catecholaminesmentioning

confidence: 95%

Section: Catecholaminesmentioning

confidence: 99%

Amino acid transport in isolated rat hepatocytes

Kilberg

1982

J. Membrain Biol.

217

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“…1A) by the administration of low doses of propranolol and epinephrine was used (12)(13)(14). To establish the model, we first analyzed the effects of the chemical inhibition of GSIS (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose (50 mg/mouse, or as indicated) was injected intraperitoneally 3 h after treatments, followed by the collection of serum samples at the indicated times. Epinephrine at 0.1 or 1 mg/kg (Mylan, Stockholm, Sweden) and propranolol at 5 mg/kg (catalog #P0884; Sigma-Aldrich, Stockholm, Sweden) were coadministered with glucose as indicated (12)(13)(14). Blood glucose levels were measured (OnetouchUltra2; LifeScan, Milpitas, CA), and insulin and C-peptide levels were assayed in serum using ELISA (Mercodia, Uppsala, Sweden, and Crystal Chem, Downers Grove, IL, respectively).…”

Section: Glucose Tolerance Testmentioning

confidence: 99%

Dual Actions of Apolipoprotein A-I on Glucose-Stimulated Insulin Secretion and Insulin-Independent Peripheral Tissue Glucose Uptake Lead to Increased Heart and Skeletal Muscle Glucose Disposal

et al. 2016

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Apolipoprotein A-I (apoA-I) of HDL is central to the transport of cholesterol in circulation. ApoA-I also provides glucose control with described in vitro effects of apoA-I on β-cell insulin secretion and muscle glucose uptake. In addition, apoA-I injections in insulin-resistant diet-induced obese (DIO) mice lead to increased glucose-stimulated insulin secretion (GSIS) and peripheral tissue glucose uptake. However, the relative contribution of apoA-I as an enhancer of GSIS in vivo and as a direct stimulator of insulin-independent glucose uptake is not known. Here, DIO mice with instant and transient blockade of insulin secretion were used in glucose tolerance tests and in positron emission tomography analyses. Data demonstrate that apoA-I to an equal extent enhances GSIS and acts as peripheral tissue activator of insulin-independent glucose uptake and verify skeletal muscle as an apoA-I target tissue. Intriguingly, our analyses also identify the heart as an important target tissue for the apoA-I–stimulated glucose uptake, with potential implications in diabetic cardiomyopathy. Explorations of apoA-I as a novel antidiabetic drug should extend to treatments of diabetic cardiomyopathy and other cardiovascular diseases in patients with diabetes.

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“…These results indicate that insulin, but not glucagon and iso proterenol, facilitates the formation of taurine-conjugated bile acids. It has been previously reported that AI B transport in primary cultured hepatocytes is facilitated by insulin, glucagon and iso proterenol (17)(18)(19).…”

mentioning

confidence: 99%