Original Research: Potential ocular protection and dynamic observation of Polygonatum sibiricum polysaccharide against streptozocin-induced diabetic rats’ model

Abstract

Ocular complications associated with diabetes mellitus are progressive and becoming one of the most important causes of morbidity worldwide. The purpose of the study is to evaluate the protective effect of Polygonatum sibiricum polysaccharide, an important component of Polygonatum sibiricum, on ocular complications in streptozotocin-induced diabetes mellitus rats. Sprague Dawley rats were made diabetic with streptozotocin(60 mg/kg, i.v.) and then the rats were treated with Polygonatum sibiricum polysaccharide 200, 400 and 800 mg/kg.d by gavage for 12 weeks. Biochemical analysis indicated that Polygonatum sibiricum polysaccharide lowered the levels of fasting blood glucose and glycated hemoglobin in blood and elevated the levels of insulin and C-peptide in plasma of diabetes mellitus rats in a dose-dependent manner. Physical measurements revealed that Polygonatum sibiricum polysaccharide improved clinical symptoms of polydipsia, polyphagia, polyuria and weight loss in diabetes mellitus rats. The content of malondialdehyde and activity of superoxide dismutase in plasma were determined, and the data showed Polygonatum sibiricum polysaccharide suppressed oxidative stress reaction. Lens opacification was observed using slit lamp illumination, and the data showed Polygonatum sibiricum polysaccharide delayed cataract progression in a dose-dependent manner. Electroretinogram showed Polygonatum sibiricum polysaccharide treatment reversed the decrease of electroretinogram b and OPs2 waves' amplitudes. Flash-visual evoked potential test indicated that the peak time of P2 wave was prolonged, and the amplitude of N2-P2 was lowered in diabetes mellitus group, and Polygonatum sibiricum polysaccharide suppressed these changes. Fundus fluorescein angiography showed Polygonatum sibiricum polysaccharide alleviated the retinal vasculopathy in a dose-dependent manner. In conclusion, these results suggest that the administration of Polygonatum sibiricum polysaccharide slows the progression of diabetic retinopathy and cataract through alleviating hyperglycemia and reducing oxidative stress in streptozotocin-induced diabetes mellitus rats.

Polygonatum sibiricum polysaccharide potentially attenuates diabetic retinal injury in a diabetic rat model

“…Based on our previous studies, PSP restrained DR progression and improved visual function in experimental diabetic rats. In the present study, we further investigated the protective effect of PSP against diabetes‐induced retinal injury and its possible underlying mechanisms.…”

“…Based on our previous studies, PSP restrained DR progression and improved visual function in experimental diabetic rats 9 .…”

Protective effects of Polygonatum sibiricum polysaccharide on acute heart failure in rats

“…The main active ingredient of Rhizoma polygonati is the Polygonatum sibiricum polysaccharide (PSP) 5 . Studies show that, Rhizoma polygonati has the anti-tumor, antioxidant, anti-inflammatory, antibacterial, blood glucose and lipid regulating, anti-virus, immune enhancing functions [6][7][8][9] . It is found that, the bioactive polysaccharide can improve the cardiac function of model animals 10,11 .…”

Shenduning Granule Attenuates Renal Injury from Oxidative Stress through the Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Response Element Pathway

“…Furthermore, it can resolve phlegm, reinforce the kidney, and strengthen bones in compatibility with other medicines [21]. Polygonatum sibiricum can suppress oxidative stress reactions [22] and improve general behavioral indicators and immunologic functions, such as ameliorating yin deficiency symptoms (e.g., dysphoria in chestpalms-soles, weight loss, soreness and weakness of the waist and knees) [23]. The Chinese herbal prescription shenduning granules have the effect of reinforcing qi, warming the yang, and totally dispersing blood stasis.…”