ORIGINAL ARTICLE: Poly‐Immunoglobulin Receptor‐Mediated Transport of IgA into the Male Genital Tract is Important for Clearance of <i>Chlamydia muridarum</i> Infection

Cunningham, Kyle W.; Carey, Alison J.; Finnie, Jane M.; Bao, Shisan; Coon, Charmere; Jones, Russell C.; Wijburg, Odilia L. C.; Strugnell, Richard A.; Beagley, Kenneth W.

doi:10.1111/j.1600-0897.2008.00637.x

Cited by 28 publications

(27 citation statements)

References 47 publications

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“…The role of IgA in chlamydial infections remains controversial. Indeed, while the absence of IgA in IgA-deficient mice seems to have no effect on clearance of primary or secondary infections, other studies have reported the effect of anti-MOMP IgA (from either vaccination with a MOMP vaccine or adoptive transfer) on reducing chlamydial infections in mice [46, 47, 51]. Thus, although our data supported a role for IgA in lowering chlamydial burden in koalas, further experiments are required to fully elucidate the role of mucosal immunity in chlamydial infections.…”

Section: Discussionmentioning

confidence: 99%

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

et al. 2017

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We assessed the effects of two different single-dose anti-Chlamydia pecorum (C. pecorum) vaccines (containing either Major Outer Membrane Protein (3MOMP) or Polymorphic Membrane Protein (Pmp) as antigens) on the immune response of a group of wild koalas. Both vaccines elicited a systemic humoral response as seen by the production of anti-chlamydial IgG antibodies in more than 90% of vaccinated koalas. A mucosal immune response was also observed, with an increase in Chlamydia-specific mucosal IgG and/or IgA antibodies in some koalas post-vaccination. Both vaccines elicited a cell-mediated immune response as measured by the production of the cytokines IFN-γ and IL-17 post-vaccination. To determine the level of protection provided by the vaccines under natural conditions we assessed C. pecorum infection loads and chlamydial disease status of all vaccinated koalas pre- and post-vaccination, compared to a non-vaccinated cohort from the same habitat. The MOMP vaccinated koalas that were infected on the day of vaccination showed significant clearance of their infection at 6 months post-vaccination. In contrast, the number of new infections in the PMP vaccine was similar to the control group, with some koalas progressing to disease. Genotyping of the ompA gene from the C. pecorum strains infecting the vaccinated animals, identified genetic variants of ompA-F genotype and a new genotype ompA-O. We found that those animals that were the least well protected became infected with strains of C. pecorum not covered by the vaccine. In conclusion, a single dose vaccine formulated with either recombinant PmpG or MOMP can elicit both cell-mediated and humoral (systemic and mucosal) immune responses, with the MOMP vaccine showing clearance of infection in all infected koalas. Although the capability of our vaccines to stimulate an adaptive response and be protective needs to be fully evaluated, this work illustrates the necessity to combine epitopes most relevant to a large panel of variable strains with an efficient adjuvant.

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Section: Discussionmentioning

confidence: 99%

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

et al. 2017

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“…In fact, we reported that male guinea pigs which were depleted of B cells by cyclophosphamide treatment, leaving cell-mediated immunity intact, had a longer course of infection with increased pathology (26). Moreover, Cunningham and colleagues recently demonstrated that polyimmunoglobulin receptor-mediated transport of IgA played an important role in the clearance of infection from the genital tracts of male mice (2). In female guinea pigs, humoral immunity is essential for the recovery of chlamydial genital infection (25), and immune serumderived antibody contributed to protection against a genital tract infection (21).…”

Section: Discussionmentioning

confidence: 97%

Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs

Wang

Nagarajan²,

Hennings

et al. 2010

Infect Immun

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Very little is known about the host response to chlamydial genital infection in the male, particularly about the nature of the local response in the urethra. In this study, the pathological and immunologic responses to urethral infection of the male guinea pig with Chlamydia caviae (Chlamydophila caviae) were characterized both during a primary infection and following a challenge infection. A dose-response experiment found that the 50% infectious dose for male urethral infection was 78 inclusion-forming units. The histopathologic response was similar to that of the female, with an initial acute inflammatory response followed by a chronic inflammatory response and plasma cell infiltration.

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“…We surmise that the induction of IgA at mucosal sites is also important for protection against chlamydial infection in male koalas. Indeed, in the murine model higher levels of IgA in the prostatic fluid correlates with a reduction of Chlamydia infection in vitro and in vivo [32]. However, reagents to measure the induction of IgA at mucosal sites are not yet available for the koala.…”

Section: Discussionmentioning

confidence: 98%

Comparison of subcutaneous versus intranasal immunization of male koalas (Phascolarctos cinereus) for induction of mucosal and systemic immunity against Chlamydia pecorum

Waugh

Andrew

Rawlinson³

et al. 2015

Vaccine

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ORIGINAL ARTICLE: Poly‐Immunoglobulin Receptor‐Mediated Transport of IgA into the Male Genital Tract is Important for Clearance of Chlamydia muridarum Infection

Abstract: These findings indicate that a Chlamydia vaccine that induces neutralizing IgA in the prostate will aid in the protection against infection in males.

Cited by 28 publications

References 47 publications

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

Immunization of a wild koala population with a recombinant Chlamydia pecorum Major Outer Membrane Protein (MOMP) or Polymorphic Membrane Protein (PMP) based vaccine: New insights into immune response, protection and clearance

Local Host Response to Chlamydial Urethral Infection in Male Guinea Pigs

Comparison of subcutaneous versus intranasal immunization of male koalas (Phascolarctos cinereus) for induction of mucosal and systemic immunity against Chlamydia pecorum

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