Original article Pharmacokinetic profile of rutin after intramuscular administration in rats favours its in vivo anti-inflammatory activity in carrageenaninduced rodent model of inflammation

Abstract

The stu dy was planned to evaluate pharmacokinetic profile and in vivo anti-inflammatory property of rutin following intramuscular administration (100 mg/kg, intramuscular) in rats. Carrageenan-induced paw edema assay was carried out separately to study in vivo anti-inflammatory property of rutin. The plasma rutin concentration was assayed using High Performance Liquid Chromatography (HPLC). The pharmacokinetic parameters like the maximum plasma drug concentration (C max ), time for maximal concentration (T max ), elimination half-life (t ½ ), apparent volume of distribution (Vd (area) ), total body clearance (Cl (B) ) and mean residence time (MRT) were 21.11  0.46 g/ml, 1.83 ± 0.17 h, 9.11  1.50 h, 16.34  2.32 l/kg, 1.27  0.04 l/h/kg and 4.79  0.55 h, respectively. The drug concentration of 0.21  0.02 g/ml in plasma was detected at 24 h. In carrageenan-induced paw edema assay, rutin (100 mg/kg) significantly decreased edema volume from 1 to 6 h in comparison to carrageenan group and vehicle group. Per cent inhibition of inflammation after 6 h of rutin administration was 29.94 ± 1.49. Intramuscular administration of rutin produced satisfactory pharmacokinetic profile with promising in vivo anti-inflammatory activity in rats.

Anti-inflammatory activity of Syzygium aromaticum (L.) Merrill & Perry oil in carrageenan-induced paw edema in female rats

“…Reported studied on the in vivo anti-inflammatory effect of ethanolic stem bark extract of Cordia africana was carried out using carrageenan-induced paw edema model in rats (Tijjani et al, 2015), Similarly, Sridevi et al (2017) also reported topical anti-inflammatory activity of an herbal gel containing solanesol extracted from tobacco scrap using carrageenan induced paw edema model in albino wistar rats. Likewise, recently the anti-inflammatory activity of rutin (100 mg/kg) and meloxicam (5 mg/kg) following its intramuscular administration was also assessed using carrageenan-induced paw edema model in rats (Modi et al, 2019).…”

GPIIb/IIIa Receptor Targeted Rutin Loaded Liposomes for Site-Specific Antithrombotic Effect

“…An aliquot of 10 μL of each sample was injected onto the HPLC column, and recorded data were compared with the calibration curve. A graph of plasma drug concentration vs time was plotted for the determination of pharmacokinetics parameters of RUT in liposomal formulations. , …”