Original article Neuroprotective properties of ciliary neurotrophic factor on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells

Wang, Ke; Zhou, Fanfan; Zhu, Xue; Zhang, Kai; Huang, Biao; Zhu, Lan; Zhu, Ling

doi:10.5114/fn.2014.43783

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…ATRA, a vitamin A derivative, plays an essential role in the development of the nervous system (Wenker et al 2010). ATRA is considered as an efficient inducer of neuronal differentiation in neuroblastoma cells; ATRA also induces these cells to obtain phenotypes, such as neurite outgrowth and morphological changes functionally and electrically similar to neurons (Sakane & Shidoji 2011;Wang et al 2014c). Among several human neutoblastoma cell lines, SH-SY5Y is the most widely used in vitro neuronal differentiation (Sallmon et al 2010).…”

Section: Discussionmentioning

confidence: 99%

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

Wang

Zhang

Han

et al. 2016

Pharmaceutical Biology

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Context In clinical practice, the promotion of neuron survival is necessary to recover neurological functions after the onset of stroke. Objective This study aimed to investigate the post-ischaemic neuroprotective effect of SMND-309, a novel metabolite of salvianolic acid, on differentiated SH-SY5Y cells. Materials and methods SH-SY5Y cells were differentiated by pre-treating with 5 mM all-transretinoic acid for 6 d. The differentiated SH-SY5Y cells were exposed to oxygen-glucose deprivation (OGD) for 2 h and reperfusion (R) for 24 h to induce OGD/R injury. After OGD injury, differentiated SH-SY5Y cells were treated with or without SMND-309 (5, 10, 20 mM) for another 24 h. Cell viability was detected through Cell counting kit-8 assay and lactate dehydrogenase leakage assay. Apoptosis was evaluated through flow cytometry, caspase-3 activity assay. Changes in protein levels were assessed through Western blot. Results SMND-309 ameliorated the degree of injury in the differentiated SH-SY5Y cells by increasing cell viabilities (5 mM, 65.4% ± 4.1%; 10 mM, 69.8% ± 3.7%; 20 mM, 75.3% ± 5.1%) and by reducing LDH activity (20 mM, 2.5 fold) upon OGD/R stimulation. Annexin V-fluorescein isothiocyanate/propidium iodide staining results suggested that apoptotic rate of differentiated SH-SY5Y cells decreased from 43.8% induced by OGD/R injury to 19.2% when the cells were treated with 20 mM SMND-309. SMND-309 significantly increased the Bcl-2 level of the injured differentiated SH-SY5Y cells but decreased the caspase-3 activity of these cells by 1.6-fold. In contrast, SMND-309 did not affect the Bax level of these cells. SMND-309 evidently increased the protein expression of BDNF when Akt and CREB were activated. This function was antagonized by the addition of LY294002. Conclusion SMND-309 can prevent neuronal cell death in vitro. This process may be related to the activation of the PI3K/Akt/CREB-signalling pathway.ARTICLE HISTORY

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Section: Discussionmentioning

confidence: 99%

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

Wang

Zhang

Han

et al. 2016

Pharmaceutical Biology

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“…4) [46][47][48]. CNTF induced PI3K/Akt pathway was shown to be important in survival and neurite growth of neuroblastoma cells and responsible for CNTF effect on skeletal muscle glucose uptake [49][50][51]. MAPK-ERK1/2 pathway was demonstrated to play an important role in CNTF induced neuronal survival in stationary hypothalamic cultures [50].…”

Section: Cntf Receptors and Signaling Pathwaysmentioning

confidence: 99%

Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies

Pasquin

Sharma

Gauchat

2015

Cytokine & Growth Factor Reviews

111

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“…SH-SY5Y cells differentiate primarily to a cholinergic neuron phenotype in response to at least 7 days of retinoic acid treatment, typically at a concentration of 10 μM . Specifically, neuronal differentiation involves the formation and extension of neuronal processes, induction of neuron-specific enzymes, and production of neurotransmitters. − Studies frequently use either undifferentiated − and retinoic acid-differentiated − SH-SY5Y cells. Some studies have compared undifferentiated and differentiated SH-SY5Y cells, but they highlight only specific proteins and pathways .…”

Section: Introductionmentioning

confidence: 99%

Temporal Proteomic Profiling of SH-SY5Y Differentiation with Retinoic Acid Using FAIMS and Real-Time Searching

2020

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The SH-SY5Y cell line is often used as a surrogate for neurons in cell-based studies. This cell line is frequently differentiated with all-trans retinoic acid (ATRA) over a 7-day period, which confers neuron-like properties to the cells. However, no analysis of proteome remodeling has followed the progress of this transition. Here, we quantitatively profiled over 9400 proteins across a 7-day treatment with retinoic acid using state-of-the-art mass spectrometry-based proteomics technologies, including FAIMS, real-time database searching, and TMTpro16 sample multiplexing. Gene ontology analysis revealed that categories with the highest increases in protein abundance were related to the plasma membrane/extracellular space. To showcase our data set, we surveyed the protein abundance profiles linked to neurofilament bundle assembly, neuron projections, and neuronal cell body formation. These proteins exhibited increases in abundance level, yet we observed multiple patterns among the queried proteins. The data presented represent a rich resource for investigating temporal protein abundance changes in SH-SY5Y cells differentiated with retinoic acid. Moreover, the sample preparation and data acquisition strategies used here can be readily applied to any analogous cell line differentiation analysis.

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Original article Neuroprotective properties of ciliary neurotrophic factor on retinoic acid (RA)-predifferentiated SH-SY5Y neuroblastoma cells

Cited by 8 publications

References 26 publications

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

SMND-309 promotes neuron survival through the activation of the PI3K/Akt/CREB-signalling pathway

Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies

Temporal Proteomic Profiling of SH-SY5Y Differentiation with Retinoic Acid Using FAIMS and Real-Time Searching

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