Original Article: Molecular and demographic analysis of respiratory syncytial virus infection in patients admitted to King Chulalongkorn Memorial Hospital, Thailand, 2007

Abstract

Please cite this paper as: Boonyasuppayakorn et al. (2010) Molecular and demographic analysis of respiratory syncytial virus infection in King Chulalongkorn Memorial Hospital admitted patients, Thailand, 2007. Influenza and Other Respiratory Viruses 4(5), 313–323. Objectives: To preliminary preview the molecular character and its possible clinical correlation of RSV subgroups in Thailand. Design: Cross‐sectional analytic design. Setting: Admitted acute lower respiratory tract infection patients of King Chulalongkorn Memorial Hospital, Bangkok, during Jun–Dec, 2007 were recruited. Sample: Nasopharyngeal aspirations were collected. Main outcome measures: All samples were analyzed for the presence of RSV glycoprotein G gene by reverse transcription PCR. Molecular character of each subgroup was determined by sequencing. Admission records were also analyzed for clinical correlations. Results: Equal infectivity and severity of both RSV subgroups to the patients was shown. Mixed infection was shown to be as common as each single infection, higher than previously reported. GA2 of subgroup A and BA‐IV of subgroup B were the most widespread genotypes and showed their monophyletic origins. From admission records, either type of infection did not show significantly preference in demographic record or clinical severity. Comorbidity, however, was statistically significant that more congenital heart disease was found in negative RSV cases, while more chronic pulmonary disease was in positive cases. Nevertheless, the clinical severity was insignificantly different suggesting that only patients with chronic pulmonary underlying were prone to be infected with RSV. Conclusions: This preliminary RSV study showed prevalence of subgroups, types of infection, and common genotypes in an epidemic, uncorrelated to demography or clinical severity.

Molecular Characterization of Circulating Respiratory Syncytial Virus (RSV) Genotypes in Gilgit Baltistan Province of Pakistan during 2011-2012 Winter Season

“…The BA genotype was subsequently detected in Japan in 2003 [44] later in China [41], Thailand [37,46,47], South Africa [9], Brazil [52] and India [8], becoming the predominant HRSV-B genotype in circulation worldwide. All the group B strains carry the characteristic 60 nucleotide duplication, conserved stop codons, and a six nucleotide deletion in HVR1 [13,30,41,48].…”

Epidemiology and Diagnosis of Human Respiratory Syncytial Virus Infections

“…Similarly, an outbreak in Turkey indicated that subgroup B was highly dominant (Guney et al, 2004). In Thailand, equal infectivity and severity of both RSV subgroups has been shown ( Boonyasuppayakom et al, 2007). Such knowledge has yet to be ascertained for the Middle Eastern countries.…”

Molecular Epidemiology and Disease Severity of Human Respiratory Syncytial Virus in Vietnam

“…GA2 was the most common genotype of RSV group A found around the world and has persisted for many years [8], [30], [31], [33], [34]. On the other hand, the G protein gene variable region of one Vietnamese strain was identical to strains from different parts of the world, including Thailand, Scotland, and Brazil.…”