Origin, timing, and gene expression profile of adventitious rooting in Arabidopsis hypocotyls and stems

Abstract

We have developed an efficient rooting protocol using half-strength Lepoivre medium for studying AR formation in stems, traced back the cellular AR origins in stems, and correlated expression of rooting-related genes with root initiation sites.

1,3-di(benzo[d]oxazol-5-yl)urea acts as either adventitious rooting adjuvant or xylogenesis enhancer in carob and pine microcuttings depending on the presence/absence of exogenous indole-3-butyric acid

“…In all cases, AR formation involves the canalization of somatic cells into a new organogenic program. Some endogenous factors, hormones and/or hormonal balance, tissue maturity, expression of specific regulatory genes, have been recognized as fundamental factors in this regeneration scenario (Busov et al 2009;Abarca and Diaz-Sala 2009;da Costa et al 2013;Della Rovere et al 2013, 2015Legué et al 2014;Pacurar et al 2014;Welander et al 2014). Xylogenesis consists of the formation of xylary cells.…”

Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root

“…The anatomical structure of Arabidopsis FS has been addressed previously (Welander et al 2014). The FS consists of one row of epidermis cells as outermost layer and a few rows of cortex cells.…”

“…Does this change at anatomical level influence adventitious rooting? Welander et al (2014) showed that in FS explants starch sheath cells adjacent to the phloem are the main origins of ARs. It seems, therefore, that the formation of secondary phloem in flooded explants increases the area in which starch sheath cells are adjacent to the phloem parts.…”

Effect of Auxins and Associated Metabolic Changes on Cuttings of Hybrid Aspen

“…In addition, the interdependent physiological stages of the rooting process are also associated with changes in endogenous auxin concentrations [10]. Some endogenous factors, hormones and/or hormonal balance, tissue maturity, expression of specific regulatory genes, have also been recognized as fundamental factors in this regeneration scenario [11][12][13][14][15][16]. However, the combined effects of auxin type, auxin concentration, and the accompanying biochemical changes on 2 of 12 rooting of hybrid aspen cuttings have not been reported.…”