Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root

Massoumi, Mehdi; Krens, Frans A.; Visser, Richard G. F.; Klerk, G.J.M. de

doi:10.1007/s11240-017-1244-1

Cited by 13 publications

(11 citation statements)

References 53 publications

(59 reference statements)

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“…In the absence of exogenous auxin, seedlings spontaneously developed relatively low numbers of roots, although these displayed considerable length, confirming the promptness of Arabidopsis hypocotyls to develop adventitious roots in this experimental system, which combines pre-etiolation and flooding to induce AR development (Correa et al, 2012). Both of these environmental factors may act as triggers for rooting by affecting variables such as phytohormonal balance, auxin transport and carbohydrate accumulation (Massoumi et al, 2017;Lu et al, 2017). Auxins stimulate adventitious rooting in the first stages of the process, but repress later developmental stages (De Klerk et al, 1999;Verstraeten et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Comparative adventitious root development in pre-etiolated and flooded Arabidopsis hypocotyls exposed to different auxins

Costa

Gaeta

Mariath

et al. 2018

Plant Physiology and Biochemistry

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Adventitious roots (ARs) emerge from stems, leaves or hypocotyls, being strategic for clonal propagation. ARs may develop spontaneously, upon environmental stress or hormonal treatment. Auxins strongly influence AR development (ARD), depending on concentration and kind. However, the role of different types of auxin is rarely compared at the molecular level. Rooting triggered by light exposure and flooding was examined in intact etiolated Arabidopsis thaliana hypocotyls treated with distinct auxin types. Morphological aspects, rooting-related gene expression profiles, and IAA immunolocalization were recorded. NAA and 2,4-D effects were highly dose-dependent; at higher concentrations NAA inhibited root growth and 2,4-D promoted callus formation. NAA yielded the highest number of roots, but inhibited elongation. IAA increased the number of roots with less interference in elongation, yielding the best overall rooting response. IAA was localized close to the tissues of root origin. Auxin stimulated ARD was marked by increased expression of PIN1 and GH3.3. NAA treatment induced expression of CYCB1, GH3.6 and ARF8. These NAA-specific responses may be associated with the development of numerous shorter roots. In contrast, expression of the auxin action inhibitor IAA28 was induced by IAA. Increased PIN1 expression indicated the relevance of auxin efflux transport for focusing in target cells, whereas GH3.3 suggested tight control of auxin homeostasis. IAA28 increased expression during IAA-induced ARD differs from what was previously reported for lateral root development, pointing to yet another possible difference in the molecular programs of these two developmental processes.

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Section: Discussionmentioning

confidence: 99%

Comparative adventitious root development in pre-etiolated and flooded Arabidopsis hypocotyls exposed to different auxins

Costa

Gaeta

Mariath

et al. 2018

Plant Physiology and Biochemistry

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“…The high percentage increase in stover dry weight and root dry weight was not only a result of the role of B. subtilis and Trichoderma sp but also as a form of optimal response from plants. Soybean is responsive to light [9] which is manifested in optimal sugar production at the end of the photosynthesis process and plays an important role in the process of young tissue growth [23] and the process of transition from young plants to mature plants in the vegetative phase [10].…”

Section: Discussionmentioning

confidence: 99%

The Vegetative Growth Response of Detam Soybean Varieties towardsBacillus subtilisandTrichodermasp. Applications as Bio-fertilizer

Miftakhurrohmat

Sutarman

2021

E3S Web Conf.

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This study aims to determine the effect of bacterial isolates of Bacillus subtilis Bs-Sdj-01 and Trichoderma sp. Tc-Jro-02 isolates as biological fertilizer in plant growth until the end of the vegetative phase. The experiment was arranged factorially in a Completely Randomized Design (CRD) with each factor being the application of B. subtilis consisting of and without B. subtilis and the Trichoderma application consisting of with and without Trichoderma; the experiment was repeated four times. The observed variables were: plant height, stem diameter, number of leaves, leaf area, stover dry weight, root dry weight, and B. subtilis population at the end of the observation. Data were analyzed using with ANOVA and HSD tests at the 5% level. The combination of Trichoderma sp. and B. subtilis bacteria produce the highest increase in plant height, stem diameter, number of leaves, leaf area, stover dry weight, and root dry weight of soybean plants up to 35 days after planting and increase B. subtilis population grew from 1010 CFU.g-1 to be 4.43x1011 CFU.g-1. The presence of Trichoderma supports the activity of B. subtilis in the rhizosphere of the Detam variety soybean plantation.

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“…Sterilized seeds were germinated on Murashige and Skoog medium without hormones. Dark and light treatments were performed according to Masomi et al [83], with a little modification-the hypocotyl was sliced from the primary root and hypocotyl junction, but also hypocotyls were wounded for easy uptake of hormones. Seeds were given pretreatment of darkness for 4 days continuously followed by 4 days' photoperiod 16/8 h, and controlled samples were kept under 16/8 h photoperiod for 8 days.…”

Section: Plant Materialsmentioning

confidence: 99%

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

Munir

Din

Imran

et al. 2021

Forests

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The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants.

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Etiolation and flooding of donor plants enhance the capability of Arabidopsis explants to root

Cited by 13 publications

References 53 publications

Comparative adventitious root development in pre-etiolated and flooded Arabidopsis hypocotyls exposed to different auxins

Comparative adventitious root development in pre-etiolated and flooded Arabidopsis hypocotyls exposed to different auxins

The Vegetative Growth Response of Detam Soybean Varieties towardsBacillus subtilisandTrichodermasp. Applications as Bio-fertilizer

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

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