Origin of endothelial progenitors in human postnatal bone marrow

Reyes, Morayma; Dudek, Arkadiusz Z.; Jahagirdar, Balkrishna; Koodie, Lisa; Marker, Paul H.; Verfaillie, Catherine M.

doi:10.1172/jci200214327

Cited by 228 publications

(209 citation statements)

References 31 publications

(10 reference statements)

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“…The precise nature of this circulating muscle stem cell remains to be defined. Recent work suggests that satellite cells may derive at least in part from angioblastic precursors associated with embryonic vasculature and that multipotent stem cells are associated with the vasculature in adult tissues (12,50). Expression of AC133 transcripts in hematopoietic adult tissues has been attributed to a hematopoietic/endothelial progenitor with hemangioblastic properties (18).…”

Section: Discussionmentioning

confidence: 99%

Human circulating AC133+ stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle

Torrente¹,

Belicchi²,

Sampaolesi³

et al. 2004

J. Clin. Invest.

178

246

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Duchenne muscular dystrophy (DMD) is a common X-linked disease characterized by widespread muscle damage that invariably leads to paralysis and death. There is currently no therapy for this disease. Here we report that a subpopulation of circulating cells expressing AC133, a well-characterized marker of hematopoietic stem cells, also expresses early myogenic markers. Freshly isolated, circulating AC133 + cells were induced to undergo myogenesis when cocultured with myogenic cells or exposed to Wnt-producing cells in vitro and when delivered in vivo through the arterial circulation or directly into the muscles of transgenic scid/mdx mice (which allow survival of human cells). Injected cells also localized under the basal lamina of host muscle fibers and expressed satellite cell markers such as M-cadherin and MYF5. Furthermore, functional tests of injected muscles revealed a substantial recovery of force after treatment. As these cells can be isolated from the blood, manipulated in vitro, and delivered through the circulation, they represent a possible tool for future cell therapy applications in DMD disease or other muscular dystrophies.

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Section: Discussionmentioning

confidence: 99%

Human circulating AC133+ stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle

Torrente¹,

Belicchi²,

Sampaolesi³

et al. 2004

J. Clin. Invest.

178

246

View full text Add to dashboard Cite

show abstract

“…The VEGF produced in the homed circulating EPCs may augment angiogenesis and also exert a proliferative and antiapoptotic effect both on tumor and tumor ECs [29, 30]. Previous results have demonstrated the feasibility of using genetically modified EPCs as angiogenesis-selective gene-targeting vectors and the potential of this approach to mediate nontoxic and systemic antitumor responses [19, 20, 31]. Herein, we have used peripheral blood-derived EPCs as a vehicle for HIF-1α siRNA in experimental hypoxia to assess EPC differentiation and migration.…”

Section: Discussionmentioning

confidence: 99%

“…The importance of these cells in tumor growth coupled with their ability to be used for induction of neovascularization in areas of hypoxia led us to ask whether such cells could be utilized as angiogenesis-selective gene-targeting vectors. In addition to sprouting angiogenesis from neighboring resident endothelial cells (ECs), tumors also build vessels by recruitment and in situ differentiation of circulating endothelial progenitor cells (EPCs) [20]. These findings offer the possibility to exploit EPCs for cancer therapy [21].…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Hypoxia-Inducible Factor-1α and Endothelial Progenitor Cell Differentiation by Adenoviral Transfer of Small Interfering RNA in vitro

Jiang

Wang²,

Wang³

et al. 2006

J Vasc Res

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RNA interference is applied to study gene function in different organisms and in various cell types. Little is known about the effect of RNA interference on human endothelial progenitor cells (EPCs) in vitro. To address this issue, short hairpin RNA targeting the human hypoxia inducible factor-1α (HIF-1α) was transferred into human EPCs by an adenoviral vector. HIF-1α mRNA and protein expression was dramatically and specifically downregulated after adeno-small interfering RNA (siRNA)-HIF-1α infection in cells under hypoxia, a condition in which HIF-1α would have been induced. This effect persisted for at least 72 h and was accompanied by suppression of vascular endothelial growth factor (VEGF) mRNA and protein expression. The expression of endothelial cell markers CD31, VEGF receptor 2 (Flk-1) and eNOS as well as NO production were also markedly decreased. Functional studies showed HIF-1α knockdown via adenoviral siRNA transfer inhibited EPC colony formation, differentiation, proliferation and migration. These data indicate that specific gene knockdown via adenoviral transfer of siRNA is feasible in EPCs, and the effect is long-lasting. Our findings raise the possibility that such long-term modified human EPCs may be used to treat hypoxic tumor metastases that are known to be resistant to conventional therapeutic regimes.

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“…Differentiation of embryonic stem cells is associated with downregulation of Oct-4 [53, 54]. Oct-4 expression has been demonstrated in stem cells isolated from umbilical cord blood, bone marrow, hair follicles, muscle, skin, breast, pancreas, liver, amniotic fluid, as well as endothelial progenitor cells and neural stem cells [47,55,56,57,58,59,60,61,62,63,64,65,66,67,68]. These markers will be useful to localize a kidney stem cell niche and can be used to study cell lineage progression in the normal and injured kidney.…”

Section: Isolation Of Adult Kidney Stem Cellsmentioning

confidence: 99%

Do Stem Cells Exist in the Adult Kidney?

Gupta

Rosenberg²

2008

Am J Nephrol

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Adult stem cells exist in many organs and play a critical role in normal cell turnover and the response to injury. The existence of adult stem cells in the mammalian kidney remains controversial. Kidney stem cells have been isolated and characterized by many groups, often with discrepant results. This article will review the current state of knowledge regarding adult kidney stem cells and discuss future directions for kidney stem cell research.

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Origin of endothelial progenitors in human postnatal bone marrow

Cited by 228 publications

References 31 publications

Human circulating AC133+ stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle

Human circulating AC133+ stem cells restore dystrophin expression and ameliorate function in dystrophic skeletal muscle

Inhibition of Hypoxia-Inducible Factor-1α and Endothelial Progenitor Cell Differentiation by Adenoviral Transfer of Small Interfering RNA in vitro

Do Stem Cells Exist in the Adult Kidney?

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