Do Stem Cells Exist in the Adult Kidney?

Gupta, Sandeep K.; Rosenberg, Mark E.

doi:10.1159/000117311

Cited by 37 publications

(33 citation statements)

References 146 publications

(74 reference statements)

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“…In organisms such as the skate and freshwater teleost, renal stem cells persist in the adult life and form new nephrons [4,5]. There is increasing debate whether stem cells exist in the adult mammalian kidney and participate in renal regeneration following injury [6,7]. Although great advances have been made in recent years, a major limitation in the field is the lack of definitive markers for identifying stem cells present in the adult mammalian kidney.…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Sall1-Expressing Cells Present in the Adult Mouse Kidney

Abedin¹,

Imai²,

Rosenberg³

et al. 2011

Nephron Exp Nephrol

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Background: Sall1 is a transcription factor that best identifies stem cells present in the mouse embryonic kidney. Mutations in Sall1 gene in mice can lead to dysgenesis of kidney, while in humans it results in the Townes-Brocks syndrome, which is associated with the kidney agenesis. Unlike the embryonic kidney, Sall1 expression in the adult kidney is largely unknown. We hypothesized that similar to the embryonic kidney, Sall1 expression can identify stem cells present in the adult kidney. Accordingly in this study, we identified Sall1-expressing cells in the adult mouse kidney, determined their role in kidney regeneration following ischemia-reperfusion injury (IRI), and sought the effect of age on Sall1 expression. Methods and Results: By immunofluorescence Sall1-expressing cells were identified in the proximal tubule at the cortico-medullary junction and constituted 0.5% of all tubular cells. Rare Sall1-positive cells were also identified in the outer cortex and distal tubules. Sall1 expression was not seen in the glomerular, interstitial, or vascular compartments. Following IRI, 90% of Sall1-expressing cells proliferated and 5% of Sall1-positive cells showed asymmetrical cell division with one of the two adjacent Sall1-positive cells incorporating chlorodeoxyuridine (CldU). Following IRI, there was an increase in Sall1 expression at 4 and 12 h, a decrease at 5 and 10 days, and baseline expression at day 30 by quantitative polymerase chain reaction (qRT-PCR) and Western blot analysis. There was no age-related change in Sall1 expression as determined by qRT-PCR, Western blot analysis, and immunofluorescence. Conclusions: We conclude that Sall1-expressing cells are present in the adult mouse kidney, predominantly in the proximal tubules. Sall1-expressing cells proliferate following IRI and some of the Sall1-positive cells undergo asymmetrical cell division. Therefore, Sall1 is a promising marker for identification of stem cells present in the adult mouse kidney.

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Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Sall1-Expressing Cells Present in the Adult Mouse Kidney

Abedin¹,

Imai²,

Rosenberg³

et al. 2011

Nephron Exp Nephrol

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“…Oct-4 expression has been demonstrated at different locations in the human body like bone marrow, hair follicles, breast, liver, or neural stem cell (Gupta and Rosenberg 2008;Jiang et al 2002;Reyes et al 2002;Yu et al 2006) and now also in nasal polyp cells. Besides Oct-4, Sox2 is one of the transcription factors which is essential for the pluripotent cell development and maintenance of undifferentiated embryonic stem cells (Avilion et al 2003;Nichols et al 1998) and was found in the epithelium of nasal polyps.…”

Section: Discussionmentioning

confidence: 99%

Neuronal Differentiation Capability of Nasal Polyps of Chronic Rhinosinusitis

Koennecke

Böscke

Pfannerstill

et al. 2017

Arch. Immunol. Ther. Exp.

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“…12 Even organs previously considered as post-mitotic, such as the heart or kidney, are now believed to have their own stem cell compartments, which are, however, still poorly understood. 13, 14 …”

Section: Adult Stem Cellsmentioning

confidence: 99%

Biology of stem cells: an overview

Chagastelles

Nardi

2011

Kidney International Supplements

106

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Stem cells are defined as precursor cells that have the capacity to self-renew and to generate multiple mature cell types. Only after collecting and culturing tissues is it possible to classify cells according to this operational concept. This difficulty in identifying stem cells in situ, without any manipulation, limits the understanding of their true nature. This review aims at presenting, to health professionals interested in this area, an overview on the biology of embryonic and adult stem cells, and their therapeutic potential.

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Do Stem Cells Exist in the Adult Kidney?

Cited by 37 publications

References 146 publications

Identification and Characterization of Sall1-Expressing Cells Present in the Adult Mouse Kidney

Identification and Characterization of Sall1-Expressing Cells Present in the Adult Mouse Kidney

Neuronal Differentiation Capability of Nasal Polyps of Chronic Rhinosinusitis

Biology of stem cells: an overview

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