2001
DOI: 10.1128/jvi.75.3.1487-1506.2001
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Origin-Independent Assembly of Kaposi's Sarcoma-Associated Herpesvirus DNA Replication Compartments in Transient Cotransfection Assays and Association with the ORF-K8 Protein and Cellular PML

Abstract: Six predicted Kaposi's sarcoma virus herpesvirus (KSHV) proteins have homology with other well-characterized herpesvirus core DNA replication proteins and are expected to be essential for viral DNA synthesis. Intact Flag-tagged protein products from all six were produced from genomic expression vectors, although the ORF40/41 transcript encoding a primase-helicase component proved to be spliced with a 127-bp intron. The intracellular localization of these six KSHV replication proteins and the mechanism of their… Show more

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Cited by 151 publications
(196 citation statements)
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“…In some cells, staining for vPK displayed a discrete dot-like pattern. This pattern of vPK compartmentalization coincided with viral replication foci marked by the KSHV DNA PPF (ORF59) (54). In other cells, staining for vPK exhibited a more diffuse arrangement (Fig.…”
Section: Discussionmentioning
confidence: 97%
See 1 more Smart Citation
“…In some cells, staining for vPK displayed a discrete dot-like pattern. This pattern of vPK compartmentalization coincided with viral replication foci marked by the KSHV DNA PPF (ORF59) (54). In other cells, staining for vPK exhibited a more diffuse arrangement (Fig.…”
Section: Discussionmentioning
confidence: 97%
“…Given the close proximity of vPK with the KSHV replication complex, we determined whether the replication proteins of this virus are potential substrates for vPK. Eight KSHV gene products, shown to be essential for viral DNA replication and localized in the replication complex (3,30,54), were tagged with Flag epitope and expressed by baculovirus vectors. These tagged proteins were purified with Flag antibody conjugated to agarose beads and subsequently eluted with Flag peptide.…”
Section: Resultsmentioning
confidence: 99%
“…These ori-Lyts are located in the KSHV genome between K4.2 and K5 and between K12 and ORF71, respectively. These two ori-Lyts share almost identical 1.1-kb core component sequences and 600-bp GCrich repeats, which are represented as 20-bp and 30-bp tandem arrays (25).Like that of other herpesviruses, KSHV lytic DNA replication relies on virus-encoded proteins (2,38,41). Six core replication proteins, including a DNA polymerase (POL), a polymerase processivity factor (PPF), a single-stranded DNA binding protein (SSB), and a trivalent helicase-primase complex (HEL, PRI, and PAP) have been identified in the KSHV genome.…”
mentioning
confidence: 99%
“…Like that of other herpesviruses, KSHV lytic DNA replication relies on virus-encoded proteins (2,38,41). Six core replication proteins, including a DNA polymerase (POL), a polymerase processivity factor (PPF), a single-stranded DNA binding protein (SSB), and a trivalent helicase-primase complex (HEL, PRI, and PAP) have been identified in the KSHV genome.…”
mentioning
confidence: 99%
“…The K8.1 gene deletion spanned the K8.1 ORF immediately after the initiation codon (ATG) and extended to approximately 90 bases upstream of the K8.1 termination codon. KSHV ORF50, specifying the major lytic transactivator RTA, and the K8 ORF specifying K-bZIP, which has been suggested to act either as a repressor of lytic replication (23,28) or as a replication activator protein (59), lie immediately 5Ј to the K8.1 gene. All three genes code for mRNAs that utilize the same polyadenylation signal immediately downstream of K8.1.…”
mentioning
confidence: 99%