Origin and Improvement of Spawn of the Cultivated Mushroom<i>Agaricus brunnescens</i>Peck

Antonio, San; James, P

doi:10.1002/9781118060797.ch3

Cited by 2 publications

(2 citation statements)

References 53 publications

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“…The spawn of all the oyster mushroom species and the mutants, collected from facility at IIHR Bangalore, was made on boiled and sterilized sorghum grains by inoculating culture pieces from 10 days old culture plates and incubated at 25±2°C (San Antonio 1984). Cultivation of the species was carried out using pasteurized paddy straw (steam pasteurization at 80±2°C) as substrate (Bano and Srivastava 1962).…”

Section: Mushroom Cultivationmentioning

confidence: 99%

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

2012

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Methanolic extracts of four cultivated edible mushrooms of Pleurotus spp. namely Pleurotus florida, Pleurotus sajor-caju, Pleurotus cystidiosus and Pleurotus djamor along with the sporeless/low sporing mutants of Pleurotus florida, and Pleurotus sajor-caju were analyzed for their antioxidant activity using different chemical assays. The electrochemical behaviors of these extracts were also analyzed using cyclic voltammetry and differential pulse voltammetry. Results showed that scavenging effects on 2,2-diphenyl-1-picrylhydrazyl radicals were good (73.3-42.4 %) at 1.5 mg/ml. At 12 mg/ml, the reducing powers (2.54-1.71) and chelating effects on ferrous ions (56.0-78.5 %) were excellent. H 2 O 2 scavenging abilities at 1.5 mg/ml showed a wide range (20.0-85.4 %). Scavenging of superoxide radicals were excellent and were found to be in the range of 61.1-90.0 % at 16 mg/ml concentration. FRAP results were in the range of 1.20 -0.98 at 16 mg/ ml. Total phenolic and total flavonoid contents of the methanolic extracts ranged from 22.67 to 36.03 mg/g and 1.19-2.94 μg/g respectively. The study assessed the amount of variation in antioxidant activities exhibited by different cultivated species and their sporeless/low sporing mutants.

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Section: Mushroom Cultivationmentioning

confidence: 99%

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

2012

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“…The spawn quality is an important factor that affects the final yield in the mushroom production. At present, a variety of different substrates such as: manure, tobacco stems and wheat, rye or millet grains have been successfully used in the spawn preparation (Salmones et al, 1999;San Antonio, 2011).…”

Section: Introductionmentioning

confidence: 99%

Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

Ortiz¹,

Colavolpe²,

Albertó³

2017

Afr. J. Biotechnol.

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Spawn quality is an important factor that affects the final yield in mushroom production. One of the most common risks is the contamination of substrates and spawn by mitosporic fungi. Some species do not easily grow on grain substrate as traditional species do and these find it real difficult to produce spawn. A method was evaluated to produce spawn for mushroom production based on aseptically encapsulated mycelium using alginate beads. Strains of four selected edible mushrooms were cultivated in liquid medium, mycelium was harvested by filtration. Subsequently, it was blended with 100 mL of 2% (w/v) sodium alginate solution or with the alginate solution with the addition of 2% (w/v) yeast extract solution or with the addition of 2% (w/v) meat extract solution. An electronic mixer was used to disaggregate the mycelium and generate a homogeneous suspension. To obtain the artificial spawn, the cells suspensions were dripped in 200 mL of 500 mM CaCl 2 solution using a cut micro-tip. Beads obtained were inoculated in potato dextrose agar Petri dishes and in substrate (wheat straw or Poplar sawdust) to evaluate the growth. Tests carried out using this method, demonstrated that the incorporation of a suitable nutrient increases the ability of selected mushroom to emerge from the beads. Mycelia colonization tests were carried out on sterilized substrate, one using alginate beads and another one using the traditional spawn as inoculum. No significant differences according to t-test (p<0.05) were shown. However, spawning production based on alginate beads can be performed in only a few days, whereas the traditional method requires more time. The method proposed here represents an antecedent to improve spawn generation for future industrial applications, for further studies and for other uses as mycelium inoculant.

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Origin and Improvement of Spawn of the Cultivated MushroomAgaricus brunnescensPeck

Cited by 2 publications

References 53 publications

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

Antioxidant and electrochemical properties of cultivated Pleurotus spp. and their sporeless/low sporing mutants

Artificial spawn generation based on alginate encapsulated mycelium as inoculum for mushroom cultivation

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