Origin and Homing of Intestinal IgA Antibody-secreting Cells

Lamm, Michael E.; Phillips‐Quagliata, Julia M.

doi:10.1084/jem.20011910

Cited by 54 publications

(37 citation statements)

References 15 publications

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“…Significant differences against vehicle control group are represented as *p \ 0.05, **p \ 0.01, or ***p \ 0.001 as IL-4, IL-5, IL-6, and IL-10 which are particularly efficient helpers for antibody production. Especially IL-4 and IL-10 relate to increase in IgA production by promoting expansion of IgA class-switched B cells and their differentiation into IgA-secreting plasma cells (Abbas et al 1991;Chen and Cerutti 2010;Lamm and Phillips-Quagliata 2002;Mora and von Andrian 2008). Cytokines also have profound inhibitory effects that serve to regulate the immune responses.…”

Section: Resultsmentioning

confidence: 99%

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

et al. 2013

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Bengkoang (Pachyrhizus erosus (L.) Urban) is one of the most popular edible root vegetables in Indonesia. Bengkoang contains fairly large amounts of carbohydrates and crude fiber. The purpose of this research is to evaluate the immunomodulatory effect of the bengkoang fiber extract (BFE) in vitro and in vivo. BFE was prepared by heating the powder of bengkoang fiber suspended in distilled water at 121°C for 20 min. BFE facilitated IgM production by the human hybridoma cell line HB4C5 cells. In addition, production of IgM, IgG, and IgA by mouse primary splenocytes was facilitated by BFE in a dose-dependent manner. BFE also significantly facilitated production of both interleukin-5 and interleukin-10 by splenocytes. Immunoglobulin production by lymphocytes from the spleen, Peyer's patch, and mesenteric lymph node were significantly activated by oral administration of BFE to mice for 14 days. The serum immunoglobulin levels of IgG, IgM, and IgA were also significantly enhanced. Furthermore, cytokine production by lymphocytes from the spleen, Peyer's patch, and mesenteric lymph node were also facilitated by oral administration of BFE. These results suggest that BFE has positive effects on the immune system in vitro and in vivo.

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Section: Resultsmentioning

confidence: 99%

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

et al. 2013

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“…Reduced numbers of IgA plasma cells in the intestinal lamina propria of HIV-1-infected patients may also result from defects in homing of B cells as they traffic from the inductive Peyer's patches to the effector lamina propria (80,81). Such defects could involve altered expression of the receptor-ligand pairs responsible for initial adhesion or recruitment of lymphocytes to the mucosa (82).…”

Section: Discussionmentioning

confidence: 99%

Mucosal Plasma Cell Repertoire During HIV-1 Infection

Scamurra

Nelson

Lin

et al. 2002

The Journal of Immunology

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Impaired development of local Ab responses may predispose HIV-1-infected patients to an increased rate, severity, and duration of mucosal infections. We characterized the repertoire of Ig-producing cells in the intestinal effector compartment (the lamina propria) of HIV-1-infected (n = 29) and seronegative control (n = 27) subjects. The density of Ig-producing cells per area was similar in both groups. However, the proportions of IgA-producing cells were lower in both the duodenum and colon from HIV-1-infected patients compared with those of control subjects (p < 0.05), with compensatory increases in IgG-producing cells in the colon and IgM-producing cells in the duodenum. Similarly, among Abs in the lumen the proportions of IgA were also decreased and the proportions of IgG were increased among HIV-1-infected patients. On a molecular level, VH gene repertoire analyses by RT-PCR revealed comparable proportions of the VH3 family among duodenal IgA transcripts (50–53%) from both groups. VH3 expression was decreased only for IgM among patients with advanced HIV-1 disease (n = 6) compared with that of control subjects (n = 8) (48 ± 8 vs 62 ± 13%; p < 0.01). Moreover, the frequencies of individual IgM and IgA VH3 genes were comparable in each group, including rates of putative HIV-1 gp120-binding VH3 genes (V3-23, V3-30, V3-30/3-30.5). We conclude that, despite a decrement in local IgA producing cells, the density and molecular VH repertoire of mucosal plasma cells are relatively intact among patients with HIV-1 infection. These data suggest that HIV-1-infected patients use functional regulatory mechanisms to provide sufficient VH diversity and effective induction and differentiation of mucosal B cells.

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“…Furthermore, we found that upon viral challenge of vaccinated mice, the ASC response in the spleen, bone marrow and lungs consisted mainly of IgG and IgA ASC, with low numbers of IgM-secreting cells. This indicates a class switch to IgA as a result of respiratory tract infection [31].…”

Section: Discussionmentioning

confidence: 99%

Two Doses of Parenterally Administered Split Influenza Virus Vaccine Elicited High Serum IgG Concentrations which Effectively Limited Viral Shedding upon Challenge in Mice

2005

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We have previously found that whole influenza virus vaccine induced a more rapid and stronger humoral response, particularly after the first dose of vaccine, than split virus vaccine in mice. In this study, we have evaluated the protective efficacy of whole and split influenza virus vaccines in mice using a nonlethal upper respiratory tract challenge model. We have also investigated the immunological correlates associated with no or very little viral shedding after viral challenge. Vaccination resulted in reduced viral shedding and shortened the duration of infection by at least 2 days. After one dose of vaccine, whole virus vaccine generally resulted in less viral shedding than split virus vaccine. In contrast, two doses of split virus vaccine, particularly the highest vaccine strengths of 15 and 30 mg HA, most effectively limited viral replication and these mice had high concentrations of prechallenge influenza-specific serum IgG. The vaccine formulation influenced the IgG2a/IgG1 ratio, and this IgG subclass profile was maintained upon challenge to some extent, although it did not influence the level of viral shedding. The concentration of postvaccination serum IgG showed an inverse relationship with the level of viral shedding after viral challenge. Therefore, serum IgG is an important factor in limiting viral replication in the upper respiratory tract upon challenge of an antigenically similar virus.

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Origin and Homing of Intestinal IgA Antibody-secreting Cells

Cited by 54 publications

References 15 publications

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

Immunomodulatory activity of Bengkoang (Pachyrhizus erosus) fiber extract in vitro and in vivo

Mucosal Plasma Cell Repertoire During HIV-1 Infection

Two Doses of Parenterally Administered Split Influenza Virus Vaccine Elicited High Serum IgG Concentrations which Effectively Limited Viral Shedding upon Challenge in Mice

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