Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro

Tamaki, Toru; Tono, Kayoko; Uchiyama, Yoshiyasu; Okada, Yoshinori; Masuda, Maki; Soeda, Shinji; Nitta, Masahiro; Akatsuka, Akira

doi:10.1007/s00441-010-1127-9

Cited by 9 publications

(24 citation statements)

References 59 publications

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“…This mixture was actually the same as for mouse Sk-MSCs [8], because it is difficult to divide myogenic and non-myogenic stem cells in mouse Sk-MSCs [34]. However, the results clearly indicated that synergistic effects and/or specific adverse effects were not observed in the mixed group, which showed comparable results to those of the Sk-34 group in all experiments.…”

Section: Discussionmentioning

confidence: 99%

A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery

et al. 2016

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Losses in vital functions of the somatic motor and sensory nervous system are induced by severe long-gap peripheral nerve transection injury. In such cases, autologous nerve grafts are the gold standard treatment, despite the unavoidable sacrifice of other healthy functions, whereas the prognosis is not always favorable. Here, we use human skeletal muscle-derived stem cells (Sk-SCs) to reconstitute the function after long nerve-gap injury. Muscles samples were obtained from the amputated legs from 9 patients following unforeseen accidents. The Sk-SCs were isolated using conditioned collagenase solution, and sorted as CD34+/45- (Sk-34) and CD34-/45-/29+ (Sk-DN/29+) cells. Cells were separately cultured/expanded under optimal conditions for 2 weeks, then injected into the athymic nude mice sciatic nerve long-gap model (7-mm) bridging an acellular conduit. After 8–12 weeks, active cell engraftment was observed only in the Sk-34 cell transplanted group, showing preferential differentiation into Schwann cells and perineurial/endoneurial cells, as well as formation of the myelin sheath and perineurium/endoneurium surrounding regenerated axons, resulted in 87% of numerical recovery. Differentiation into vascular cell lineage (pericyte and endothelial cells) were also observed. A significant tetanic tension recovery (over 90%) of downstream muscles following electrical stimulation of the sciatic nerve (at upper portion of the gap) was also achieved. In contrast, Sk-DN/29+ cells were completely eliminated during the first 4 weeks, but relatively higher numerical (83% vs. 41% in axon) and functional (80% vs. 60% in tetanus) recovery than control were observed. Noteworthy, significant increase in the formation of vascular networks in the conduit during the early stage (first 2 weeks) of recovery was observed in both groups with the expression of key factors (mRNA and protein levels), suggesting the paracrine effects to angiogenesis. These results suggested that the human Sk-SCs may be a practical source for autologous stem cell therapy following severe peripheral nerve injury.

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Section: Discussionmentioning

confidence: 99%

A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery

et al. 2016

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“…Factors and their primer sets are summarized in Table 1. Detailed methods for RT-PCR were as described previously [1,21]. Briefly, sheet-pellets were lyzed and total RNA was purified using a QIAGEN RNeasy micro kit (Hilden, Germany).…”

Section: Characterization Of Stem Cell Sheet-pellets Using Rt-pcrmentioning

confidence: 99%

Functional Nerve-Vascular Reconstitution of the Bladder-Wall; Application of Patch Transplantation of Skeletal Muscle-Derived Multipotent Stem Cell Sheet-Pellets

Soeda¹

2013

J Stem Cell Res Ther

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A three-dimensional gel-patch-like nerve-vascular reconstitution system using the Skeletal Muscle-Derived Multipotent Stem Cell (Sk-MSC) sheet-pellet was applied to the reconstitution of the severely damaged bladder wall as a non-skeletal muscle tissue, but has high demand for function. The Sk-MSC sheet-pellet was prepared by the mild detachment of expanded/confluent cells in culture with EDTA, then, collected in a tube and centrifuged. The sheetpellet was pasted on the open thin-walled region of the damaged bladder wall made by myotomy (remove one-third of serosal smooth muscle layer associate with large disruptions of nerve-blood vessel networks retaining the mucosal layer). At 4 wk after transplantation, significant prevention of the reduction in the passive wall-tension, and the positive wall-contraction via electrical stimulation was observed in the transplanted group. Supporting these functional results, immunohistochemical and immunoelectron microscopic analysis revealed that the engrafted cells actively contributed to the reconstitution of blood vessels and peripheral nerves with differentiation into pericytes, endothelial cells, and Schwann cells. However, skeletal and smooth muscle formation was not observed. Thus, this method is potentially useful for the reconstitution of nerve-vascular networks in the bladder-wall to be retaining function such as passive tension and contractile function.

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“…At that time, CD45 + cells were blood stream derived hematopoietic cells, thus, they were discarded. Recently, we found that most of the colony-forming unit of Sk-DN cells showed positive for CD29 (also known as β1-integrin), thus, their positive selection as CD34 -/CD45 -/CD29 + (Sk-DN/29 + ) is a much more practical way 21) . The current sorting pattern is shown in Fig.…”

Section: The Role Of Muscle Fiber Blmentioning

confidence: 99%

“…1H). It is important to note that cells and/or myotubes in Sk-DN and Sk-34 cell cultures typically show active and spontaneous contraction supplemental movies in 10, 21,52) . More importantly, this clonal culture suggests that the formation of numerous multinucleated myotubes can be derived from single Sk-DN and Sk-34 cells (clones).…”

Section: The Role Of Muscle Fiber Blmentioning

confidence: 99%

“…Recently, interstitial myogenic cells, that are positive for the cell stress mediator PW1 and Pax7cells 19) and/or Pax3 + / MyoD + cells 20) , were also identified as non-satellite cell muscle residents during postnatal development. In addition, Sk-34 and Sk-DN cells also expressed PW1 and its downstream effector BAX mRNAs 21) . These observations strongly indicate that there are other myogenic stem cells, in addition to satellite cells, present in the interstitial spaces of skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

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Multipotency and physiological role of skeletal muscle interstitium-derived stem cells

Tamaki

2012

JPFSM

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Multipotent stem cells, other than satellite cells, that can give rise to primary myoblasts postnatally, are present in the interstitial spaces of skeletal muscle. These stem cells show differentiation potential into mesodermal and ectodermal cell lineages, and, thus, are called skeletal muscle interstitium-derived multipotent stem cells (Sk-MSCs). They are Pax7at initial isolation; and colony-forming units of these cells typically include non-adherent type myogenic cells, while satellite cells are known to be adherent in cell culture. In these colonies, both Pax7and Pax7 + myogenic cells are produced depending on asymmetric cell division. A large number of myotubes are also formed in each colony associated with putative Pax7 + satellite cells. Interestingly, Pax7-/non-adherent myogenic cells showed basal lamina (BL) formation during cell culture, whereas Px7 + myogenic cells did not. In in vivo analysis, interstitial myogenic cells showing BL formation were detected at early stages of myogenesis in the compensatory enlarged muscle, while myogenic cells in the parent fiber BL cylinder, probably satellite cells, did not form BL. Production of BL, associated with satellite cells, is essential for the in vivo establishment of new muscle fiber formation in the interstitium with, of course, innervation and capillary supply. Thus, the multipotency of Sk-MSCs that can give rise to peripheral nerve and vascular-related cells, such as Schwann cells, perineurial cells, endothelial cells, pericyte, and vascular smooth muscle cells, may have advantages over satellite cells. Therefore, the physiological role of Sk-MSCs, as a source for postnatal new muscle fiber formation (hyperplasia) and extension of nerve-vascular networks following growth and/or severe heavy resistance exercise, needs to be further investigated.

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Origin and hierarchy of basal lamina-forming and -non-forming myogenic cells in mouse skeletal muscle in relation to adhesive capacity and Pax7 expression in vitro

Cited by 9 publications

References 59 publications

A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery

A Long-Gap Peripheral Nerve Injury Therapy Using Human Skeletal Muscle-Derived Stem Cells (Sk-SCs): An Achievement of Significant Morphological, Numerical and Functional Recovery

Functional Nerve-Vascular Reconstitution of the Bladder-Wall; Application of Patch Transplantation of Skeletal Muscle-Derived Multipotent Stem Cell Sheet-Pellets

Multipotency and physiological role of skeletal muscle interstitium-derived stem cells

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