Oriented immobilisation of engineered single-chain antibodies to develop biosensors for virus detection

Torrance, L.; Ziegler, Angelika; Pittman, H. Keith; Paterson, Michael; Toth, Rachel; Eggleston, Ian M.

doi:10.1016/j.jviromet.2005.12.012

Cited by 93 publications

(46 citation statements)

References 34 publications

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“…Interfaces with regular affinity element orientation can be constructed [42][43][44]. Assuming the validity of theoretical exponential chargeto-surface distance relationships [47], it may be possible to use oriented, rigid affinity elements to build immunoFETs that detect specific analyte charges or regions of charge in preference to or exclusion of others.…”

Section: Resultsmentioning

confidence: 99%

ImmunoFET feasibility in physiological salt environments

Casal

Wen

Gupta

et al. 2012

Phil. Trans. R. Soc. A.

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Field-effect transistors (FETs) are solid-state electrical devices featuring current sources, current drains and semiconductor channels through which charge carriers migrate. FETs can be inexpensive, detect analyte without label, exhibit exponential responses to surface potential changes mediated by analyte binding, require limited sample preparation and operate in real time. ImmunoFETs for protein sensing deploy bioaffinity elements on their channels (antibodies), analyte binding to which modulates immunoFET electrical properties. Historically, immunoFETs were assessed infeasible owing to ion shielding in physiological environments. We demonstrate reliable immunoFET sensing of chemokines by relatively ion-impermeable III-nitride immunoHFETs (heterojunction FETs) in physiological buffers. Data show that the specificity of detection follows the specificity of the antibodies used as receptors, allowing us to discriminate between individual highly related protein species (human and murine CXCL9) as well as mixed samples of analytes (native and biotinylated CXCL9). These capabilities demonstrate that immunoHFETs can be feasible, contrary to classical FET-sensing assessment. FET protein sensors may lead to point-of-care diagnostics that are faster and cheaper than immunoassay in clinical, biotechnological and environmental applications.

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Section: Resultsmentioning

confidence: 99%

ImmunoFET feasibility in physiological salt environments

Casal

Wen

Gupta

et al. 2012

Phil. Trans. R. Soc. A.

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“…The binding of the target onto the immobilized phage probe, changes the resonance angle and this resonance angle shift can be used to determine the target concentration. SPR is used to develop sensitive, real-time and label-free phage biosensors to detect ␤-galactosidase (Nanduri et al, 2007a), alfatoxin B1 (Daly et al, 2002) and food pathogens (Balasubramanian et al, 2007;Nanduri et al, 2007b;Torrance et al, 2006) in small sample volumes. A disadvantage of SPR biosensors is their large size, complexity and the need of external microfluidics to transport the samples to the sensing surface.…”

Section: Transductionmentioning

confidence: 99%

“…In those studies in which SPR or QCM was applied for measuring analyte binding, phage immobilization was performed by (1) physical adsorption (Balasubramanian et al, 2007;Fu et al, 2007;Huang et al, 2009;Johnson et al, 2008;Lakshmanan et al, 2007;Nanduri et al, 2007a,b,c;Olsen et al, 2006;Wan et al, 2007), (2) reaction between phage primary amine functions and aminereactive groups (NHS ester, epoxy, isocyanate, etc.) on the sensor surface (Cerruti et al, 2009;Shabani et al, 2008;Zhu et al, 2008) or (3) thiol-gold chemisorption (Torrance et al, 2006). In contrast to the other novel recognition elements that will be discussed in the following paragraphs (nucleic acids and MIPs), immobilization of phages is either not required (fluorescence of bioluminescence measurements) or realized by simple dipcoating.…”

Section: Immobilization Of the Recognition Elementmentioning

confidence: 99%

Recent advances in recognition elements of food and environmental biosensors: A review

Dorst

Mehta

Bekaert³

et al. 2010

Biosensors and Bioelectronics

271

149

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“…These include chemically modified antibodies to increase stability [48], fragments of antibodies that exhibit high affinity [49], affibodies [50,51], and nonantibody-based recognition molecules. Each of these recognition molecules has advantages and disadvantages (Table 21.1).…”

Section: Antibody-based Detectionmentioning

confidence: 99%

Molecular Recognition Elements for Toxin and Pathogen Detection

2011

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Rapid, sensitive, and selective detection of infectious agents in food, water, the environment, and patient samples is critical for appropriate countermeasures, particularly in the event of a suspected outbreak. The biosensors should be inexpensive, portable, and easy to use, require minimal sample preparation and reagents, and should be able to detect emerging variants for wide acceptance. The vast number of biological threats, each with its own unique characteristics, as well as the stringent requirements of a biosensor, dictates a multidisciplinary approach to overcome the scientific and technological hurdles necessary to develop a "real-time" biosensor. While advances in several areas are required, one of the most critical components of a biosensor is the recognition molecule. Ultimately, the success of a biosensor is highly dependent on the specificity, sensitivity, reproducibility, and broad applicability of the recognition element. In this chapter, we review the state-of-the-art advancements in the molecular recognition of toxins and pathogens. We discuss different recognition molecules in detail with particular emphasis on the use of emerging recognition molecules that include antimicrobial peptides, carbohydrates, molecularly imprinted polymers, aptamers, and phage for detection of infectious agents. As this chapter indicates, there are advantages and limitations to each molecule. However, significant advances in the area of molecular recognition for toxins and pathogens are being made. As the field evolves, continued improvements in molecular recognition and other components of the biosensor such as transducer Chemosensors: Principles, Strategies, and Applications, First Edition. Edited by Binghe Wang and Eric V. Anslyn.

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Oriented immobilisation of engineered single-chain antibodies to develop biosensors for virus detection

Cited by 93 publications

References 34 publications

ImmunoFET feasibility in physiological salt environments

ImmunoFET feasibility in physiological salt environments

Recent advances in recognition elements of food and environmental biosensors: A review

Molecular Recognition Elements for Toxin and Pathogen Detection

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