Recent advances in recognition elements of food and environmental biosensors: A review

Dorst, Bieke Van; Mehta, Jaytry; Bekaert, Karen; Rouah-Martin, Elsa; Coen, Wim De; Dubruel, Peter; Blust, Ronny; Robbens, Johan

doi:10.1016/j.bios.2010.07.033

Cited by 277 publications

(156 citation statements)

References 176 publications

Supporting

Mentioning

149

Contrasting

Unclassified

Order By: Relevance

“…Due to their high binding affinity, the binding and immobilization of cells by LTF-MBs was also highly effective, capturing essentially all (Ͼ98%) of 10 to 10 5 CFU · ml Ϫ1 S. Typhimurium cells from the tested suspensions. Besides not needing animals for their production and having less complicated production procedures than antibodies, phage-encoded host affinity proteins also demonstrate high degrees of resistance to pH, temperature, and protease damage (60). The phage S16 LTF-MBs were unaffected by excess background flora or variable buffer pH and salt concentrations (pH 4 to 9 and 0 to 1 M NaCl).…”

Section: Discussionmentioning

confidence: 99%

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Denyes

Dunne

Steiner

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

Bacteriophage-based assays and biosensors rival traditional antibodybased immunoassays for detection of low-level Salmonella contaminations. In this study, we harnessed the binding specificity of the long tail fiber (LTF) from bacteriophage S16 as an affinity molecule for the immobilization, enrichment, and detection of Salmonella. We demonstrate that paramagnetic beads (MBs) coated with recombinant gp37-gp38 LTF complexes (LTF-MBs) are highly effective tools for rapid affinity magnetic separation and enrichment of Salmonella. Within 45 min, the LTF-MBs consistently captured over 95% of Salmonella enterica serovar Typhimurium cells from suspensions containing from 10 to 10 5 CFU · ml Ϫ1 , and they yielded equivalent recovery rates (93% Ϯ 5%, n ϭ 10) for other Salmonella strains tested. LTF-MBs also captured Salmonella cells from various food sample preenrichments, allowing the detection of initial contaminations of 1 to 10 CFU per 25 g or ml. While plating of bead-captured cells allowed ultrasensitive but time-consuming detection, the integration of LTF-based enrichment into a sandwich assay with horseradish peroxidaseconjugated LTF (HRP-LTF) as a detection probe produced a rapid and easy-to-use Salmonella detection assay. The novel enzyme-linked LTF assay (ELLTA) uses HRP-LTF to label bead-captured Salmonella cells for subsequent identification by HRP-catalyzed conversion of chromogenic 3,3=,5,5=-tetramethylbenzidine substrate. The color development was proportional for Salmonella concentrations between 10 2 and 10 7 CFU · ml Ϫ1 as determined by spectrophotometric quantification. The ELLTA assay took 2 h to complete and detected as few as 10 2 CFU · ml Ϫ1 S. Typhimurium cells. It positively identified 21 different Salmonella strains, with no cross-reactivity for other bacteria. In conclusion, the phage-based ELLTA represents a rapid, sensitive, and specific diagnostic assay that appears to be superior to other currently available tests. IMPORTANCEThe incidence of foodborne diseases has increased over the years, resulting in major global public health issues. Conventional methods for pathogen detection can be laborious and expensive, and they require lengthy preenrichment steps. Rapid enrichment-based diagnostic assays, such as immunomagnetic separation, can reduce detection times while also remaining sensitive and specific. A critical component in these tests is implementing affinity molecules that retain the ability to specifically capture target pathogens over a wide range of in situ applications. The protein complex that forms the distal tip of the bacteriophage S16 long tail fiber is shown here to represent a highly sensitive affinity molecule for the specific enrichment and detection of Salmonella. Phage-encoded long tail fibers have huge potential for development as novel affinity molecules for robust and specific diagnostics of a vast spectrum of bacteria.

show abstract

Section: Discussionmentioning

confidence: 99%

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Denyes

Dunne

Steiner

et al. 2017

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Similarly, several microbial biosensor-based asr operons, which were confirmed as important resistance systems for As and Antimonite (Sb) in E. coli (Ramanathan et al 1997), have been developed to detect the bioavailability of As (Liao and Ou 2005;Stocker et al 2003). Compared with chemical sequential extraction methods, microbial biosensor was a less expensive, faster, more convenient, and maneuverable tool to detect metal; what more significant is that it can reflect the actual bioavailability and toxicity of metal (Liao et al 2006;Stocker et al 2003;Van Dorst et al 2010).…”

Section: Introductionmentioning

confidence: 99%

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

Hou

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

An Escherichia coli arsRp::luc-based biosensor was constructed to measure the bioavailability of arsenic (As) in soil. In previous induction experiments, it produced a linear response (R 2 =0.96, P<0.01) to As from 0.05 to 5 μmol/L after a 2-h incubation. Then, both chemical sequential extraction, Community Bureau of Reference recommended sequential extraction procedures (BCR-SEPs) and E. coli biosensor, were employed to assess the impact of different long-term fertilization regimes containing N, NP, NPK, M (manure), and NPK+ M treatments on the bioavailability of arsenic (As) in soil. Per the BCR-SEPs analysis, the application of M and M+NPK led to a significant (P<0.01) increase of exchangeable As (2-7 times and 2-5 times, respectively) and reducible As (1.5-2.5 times and 1.5-2.3 times, respectively) compared with the no fertilization treated soil (CK). In addition, direct contact assay of E. coli biosensor with soil particles also supported that bioavailable As in manure-fertilized (M and M+NPK) soil was significantly higher (P<0.01) than that in CK soil (7 and 9 times, respectively). Organic carbon may be the major factor governing the increase of bioavailable As. More significantly, E. coli biosensor-determined As was only 18.46-85.17 % of exchangeable As and 20.68-90.1 % of reducible As based on BCR-SEPs. In conclusion, NKP fertilization was recommended as a more suitable regime in As-polluted soil especially with high As concentration, and this E. coli arsRp::luc-based biosensor was a more realistic approach in assessing the bioavailability of As in soil since it would not overrate the risk of As to the environment.

show abstract

“…The electrochemical approach is cost-effective because it does not require expensive instrumentation [24,25] and would allow portability [26]. FIS transduction is especially well suited to detect changes that occur in the solution-electrode interface without the need of labels.…”

Section: Introductionmentioning

confidence: 99%

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Queirós

de‐los‐Santos‐Álvarez

Noronha

et al. 2013

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

a b s t r a c tA label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins (OMPs) was developed. Two single stranded DNA sequences were tested as recognition elements and compared. The aptamer capture probes were immobilized, with and without 6-mercapto-1-hexanol (MCH) on a gold electrode. Each step of the modification process was characterized by Faradaic impedance spectroscopy (FIS). A linear relationship between the electron-transfer resistance (R et ) and E. coli OMPs concentration was demonstrated in a dynamic detection range of 1 × 10 −7 -2 × 10 −6 M. Moreover, the aptasensor showed selectivity despite the presence of other possible water contaminates and could be regenerated under low pH condition. The developed biosensor shows great potential to be incorporated in a biochip and used for in situ detection of E. coli OMPs in water samples.

show abstract

Recent advances in recognition elements of food and environmental biosensors: A review

Cited by 277 publications

References 176 publications

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

Modified Bacteriophage S16 Long Tail Fiber Proteins for Rapid and Specific Immobilization and Detection of Salmonella Cells

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

A label-free DNA aptamer-based impedance biosensor for the detection of E. coli outer membrane proteins

Contact Info

Product

Resources

About