Orientation‐Specific Attachment of Polymeric Microtubes on Cell Surfaces

Gilbert, Jonathan B.; O'Brien, Janice S.; Suresh, Harini; Cohen, Robert E.; Rubner, Michael F.

doi:10.1002/adma.201302673

Cited by 38 publications

(36 citation statements)

References 44 publications

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“…Usually, the most commonly used sacrificial templates onto which particles are synthesized are silica or polystyrene solid spheres, due to the ease of removal to achieve hollow particles [21,29]. A range of spherical and tubular nano and micro particles have been synthesized using templates of varying sizes and materials such as polystyrene sulfonate (PSS), polyallymine hydrochloride (PAH) and polyacrylic acids (PAA) [22,23,[30][31][32][33]. However, use of such particles in medicine is limited due to their reduced biocompatibility and biodegradability.…”

Section: Introductionmentioning

confidence: 99%

Design of extracellular protein based particles for intra and extra-cellular targeting

Omorphos

Kahn

Kalaskar

2015

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 99%

Design of extracellular protein based particles for intra and extra-cellular targeting

Omorphos

Kahn

Kalaskar

2015

Colloids and Surfaces B: Biointerfaces

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“…For studying the cellular behaviors in 3D microenvironment, microtubes made of biocompatible materials are highly desirable. A number of methods, including self‐assembling, self‐rolled‐up, templating, electrospinning, lithography, and direct laser writing, have been developed to fabricate different types of microtubes of varying scales. However, the processing flexibility has yet to be improved, because some of these methods could only produce microtubes with specific shapes or rough surface in fixed patterns, or some processes are complicated or inefficient.…”

Section: Introductionmentioning

confidence: 99%

Dimension‐Controllable Microtube Arrays by Dynamic Holographic Processing as 3D Yeast Culture Scaffolds for Asymmetrical Growth Regulation

Yang

et al. 2017

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Transparent microtubes can function as unique cell culture scaffolds, because the tubular 3D microenvironment they provide is very similar to the narrow space of capillaries in vivo. However, how to realize the fabrication of microtube-arrays with variable cross-section dynamically remains challenging. Here, a dynamic holographic processing method for producing high aspect ratio (≈20) microtubes with tunable outside diameter (6-16 µm) and inside diameter (1-10 µm) as yeast culture scaffolds is reported. A ring-structure Bessel beam is modulated from a typical Gaussian-distributed femtosecond laser beam by a spatial light modulator. By combining the axial scanning of the focused beam and the dynamic display of holograms, dimension-controllable microtube arrays (straight, conical, and drum-shape) are rapidly produced by two-photon polymerization. The outside and inside diameters, tube heights, and spatial arrangements are readily tuned by loading different computer-generated holograms and changing the processing parameters. The transparent microtube array as a nontrivial tool for capturing and culturing the budding yeasts reveals the significant effect of tube diameter on budding characteristics. In particular, the conical tube with the inside diameter varying from 5 to 10 µm has remarkable asymmetrical regulation on the growth trend of captured yeasts.

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“…Besides, a novel characteristic of the silica rods was discovered, that is, they cannot be favourably taken up by the cells. 31 Nevertheless, the rationale of this phenomenon is to be further claried. S3, † major portion of the rods were distributed on the membrane of the cells, while few of them were found in the cytoplasm.…”

mentioning

confidence: 99%

Surface patterned hydrogel film as a flexible scaffold for 2D and 3D cell co-culture

et al. 2016

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Herein we report a facile route for the preparation of surface patterned dynamic hydrogel films, which are not only a matrix to encapsulate one type of cell in 3D but also a substrate to support aligned aggregates of magnetic silica rods to adhere another type of cell in 2D. This enables the composite hydrogel films to be flexible scaffolds for engineering multi-cellular tissues.Chemical cues and topography of a scaffold are able to affect cell behaviour and hence the quality of tissue regeneration. 1-4 For example, cell-growth direction, such as the alignment of cells in vessel endothelium, is recognized as a key issue in controlling the structure and composition of the particular tissue. 5,6 Thus the construction of extracellular substrates is of great interest to render the engineering of an oriented cell layer. Many approaches have been developed for the fabrication of a surface with bril like pattern in order to regulate the orientation of cells during their proliferation. 7-12 However, in most of the microfabrications, the substrate for supporting the patterned surface has no additional bio-function rather than providing mechanical properties. Natural tissues are normally multi-cellular systems of two or more types of cell. Therefore in recent years, hierarchical structured double-layered scaffolds were developed, 13 for example by electrospun technique, which contain a top layer formed by aligned bres and a bottom matrix by randomly packed bres for the co-culture of two cell lines, and thereby duplicate the architecture of native tissue like the peripheral nerve. 6 Nevertheless, it is still a challenge to develop exible scaffolds for simultaneously control growth of different cells.Hydrogel is known as an ideal extracellular matrix (ECM) for culturing cell in three dimensions (3D cell culture). 3,14,15 The so nature of hydrogel can more favourably mimic the property of so tissues. Besides, hydrogel has the capacity of incorporation of bioactives, such as growth factors, to inuence the embryonic development, proliferation and even the differentiation of the encapsulated cells. [16][17][18] Hydrogel for cell co-culture was also reported. 19-21 Bi-layered and multilayered bulk hydrogels have been synthesized to mediate the formation of distinctive structure of osteochondral tissue and articular cartilage. 22,23 However, in some cases it requires the proliferation of cell in 2D and 3D concurrently instead of a 3D/3D co-culture, such as for the regeneration of tissues containing endothelial system. Recently, the modication of hydrogel surface with celladhesive patterns for directional 2D culture has been documented in literatures. 7,24-26 Nevertheless, the fabrication involves organic solvent or other toxic additives. Therefore cells must be seeded aer the fabrication and thus can no longer be entrapped inside the gel substrate and proliferated in 3D.In previous work, we developed in situ forming hydrogel based on glycol chitosan (GC) and benzaldehyde capped poly (ethylene oxide) derivatives (OHC-PEO-C...

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Orientation‐Specific Attachment of Polymeric Microtubes on Cell Surfaces

Cited by 38 publications

References 44 publications

Design of extracellular protein based particles for intra and extra-cellular targeting

Design of extracellular protein based particles for intra and extra-cellular targeting

Dimension‐Controllable Microtube Arrays by Dynamic Holographic Processing as 3D Yeast Culture Scaffolds for Asymmetrical Growth Regulation

Surface patterned hydrogel film as a flexible scaffold for 2D and 3D cell co-culture

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