Orientation of ECM protein deposition, fibroblast cytoskeleton, and attachment complex components on silicone microgrooved surfaces

A three-dimensional (3D) scaffold fabricated via electrohydrodynamic jet printing (E-jetting) and thermally uniaxial stretching, has been developed for tendon tissue regeneration in our previous study. In this study, more in-depth biological test showed that the aligned cell morphology guided by the anisotropic geometries of the 3D tendon scaffolds, leading to up-regulated tendious gene expression including collagen type I, decorin, tenascin-C, and biglycan, as compared to the electrospun scaffolds. Given the importance of geometric cues to the biological function of the scaffolds, the degradation behaviors of the 3D scaffolds were investigated. Results from accelerated hydrolysis showed that the E-jetted portion followed bulk-controlled erosion, while the unaixially stretched portion followed surface-controlled erosion. The 3D tendon scaffold exhibited consistency between the weight loss and the decline of mechanical properties, which indicated by a 65% decrease in mass with a corresponding 56% loss in ultimate tensile strength after degradation. This study not only reveals that the anisotropic geometries of 3D tendon scaffold could affect cell morphology and lead to desired gene expression toward tendon tissue but also gives an insight into how the degradation impacts geometric cues and mechanical properties of the as-fabricated scaffold. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1138-1149, 2017.

Section: Discussionsupporting

confidence: 60%

Degradation behaviors of geometric cues and mechanical properties in a 3D scaffold for tendon repair

Wong

Fuh

2017

“…COL I contains a large number of cellular binding sites that are adsorbed on PDA to mediate cell adhesion (Yeh, Chen, Shie, & Fang, 2015). It has been previously demonstrated that the substratum topography can alter FN deposition (den Braber et al, 1998). FAK plays critical roles in fibroblast adhesion signaling and the level of fibrotic response (den Braber et al, 1998;Rustad, Wong, & Gurtner, 2013).…”

Section: Discussionmentioning

confidence: 99%

Synergetic effect of chemical and topological signals of gingival regeneration scaffold on the behavior of human gingival fibroblasts

Yang

Guo

et al. 2019

Achievement of gingiva regeneration poses a substantial challenge for dental aesthetics and periodontal disease repair, but reports of a bioactive and easily available gingival regeneration scaffold are rare. Cell behaviors can be affected by multiple kinds of bioactive signals; thus, it is important to explore the effects of the chemical and topological signals of the scaffold on the behavior of human gingival fibroblasts (HGFs). We hypothesized that the synergetic effect of the chemical and topological scaffold signals were beneficial to gingival regeneration. In this study, HGF behavior on random/aligned poly (e‐caprolactone) (PCL) and polydopamine (PDA)‐coated PCL electrospun scaffolds was investigated in a common medium and in basic fibroblast growth factor (bFGF) medium. The results showed that the synergistic effect of three signals was better than that of one or two signals. The cell proliferation of the aligned scaffold group was higher than that of the random scaffold, and the PCL/PDA‐Aligned+bFGF group showed the highest cell proliferation. Even if two chemical signals were present, the HGFs still maintained an ordered distribution on the aligned scaffold. Cell differentiation and protein secretion analysis indicated that gene and protein expression of focal adhesion kinase and fibronectin were the highest in the PCL/PDA‐Aligned+bFGF group. Taken together, the chemical and topographic signals within the electrospun scaffold were considered to display a synergistic effect on HGF behaviors, suggesting the potential usefulness of the PCL/PDA‐Aligned+bFGF scaffold for gingiva tissue engineering.

“…For the orientation parameter, an angle of less than 10°with respect to the direction of the lines on a surface is considered to be highly aligned. 10,13,46 The data are summarized in Table I and plotted in Figure 6(a), where the adjoining lines signify data points that are statistically distinct. The symbol "*" indicates a significant difference where p Ͻ 0.05, Mann-Whitney Analysis; and the symbol "#" indicates significant differences where p Ͻ 0.05, Kruskal-Wallis one way Analysis with Dunn's Test for ranks.…”

Section: Mpe Patterns Direct Cell Adhesion and Spreadingmentioning

confidence: 99%

Multiphoton excited fabricated nano and micro patterned extracellular matrix proteins direct cellular morphology

Pins

Bush

Cunningham

et al. 2006

We use multiphoton excited (MPE) photochemistry to fabricate patterned extracellular matrices (ECM) and to investigate the morphology of human dermal fibroblasts adhered to the resulting photocrosslinked linear structures of fibronectin (FN), fibrinogen (FG), and bovine serum albumin (BSA). These proteins were chosen to systematically investigate the roles of topography and ECM biochemistry on cell spreading, as fibroblasts bind directly to both FN and FG at RGD sites through known integrins, whereas BSA provides no comparable ECM cues for cell binding. MPE crosslinked patterns are created from parallel linear structures 600 nm in width, 200 microm in length, and spaced by either 10 or 40 microm. Immunofluorescence staining of FN and FG was used to assay the functionality of crosslinked proteins. The metrics of orientation, elongation, and cell perimeter were used to quantitate the resulting cellular behavior on the crosslinked protein patterns. These parameters all reflect statistical differences for cells on BSA, relative to the similar statistical behavior on fibronectin and fibrinogen. Cells on the BSA patterns are constrained by physical guidance and orientation between linear structures. In contrast, cells adhered on both FN and FG had a greater propensity to spread across adjacent structures, indicating the importance of cell matrix interactions. Focal adhesion staining of cells adhered to the protein structures revealed similar trends. These findings are consistent with our hypothesis that these crosslinked matrix protein structures are expected to direct cell adhesion and spreading and that the topography and ECM cues lead to different forms of guidance.