Orientation of a Trametes versicolor laccase on amorphous carbon nitride coated graphite electrodes for improved electroreduction of dioxygen to water

Blout, Achraf; Billon, Florence; Calers, Christophe; Méthivier, Christophe; Pailleret, Alain; Perrot, Hubert; Jolivalt, Claude

doi:10.1016/j.electacta.2018.04.145

Cited by 5 publications

(11 citation statements)

References 63 publications

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“…No conclusion about the size was possible with AFM alone, but in conjugation with ellipsometry, electrochemistry and enzymatic assays it suggested a flattening of enzyme on gold surface upon adsorption . In the study already mentioned above where a laccase was immobilized on amorphous carbon nitride, correlation between AFM topography image and estimation of height profile via nanoscratching allowed detecting deformed enzymes standing flat on the surface …”

Section: Morphology Of Immobilized Enzymesmentioning

confidence: 99%

“…The attribution was realized thanks to the correlation between AFM topography image and estimation of height profile via nanoscratching. In the case of amine bond, a full layer of enzymes was formed . An Os‐polymer film decorated with gold nanoparticles was imaged by AFM prior to and after incubation in a GOx solution.…”

Section: Enzyme Localizationmentioning

confidence: 99%

“…In the case of amine bond, a full layer of enzymes was formed. [76] An Os-polymer film decorated with gold nanoparticles was imaged by AFM prior to and after incubation in a GOx solution. A clear change in the morphology of the film was observed and GOx seemed to predominantly adsorb on AuNPs rather than on polymeric domains.…”

Section: Scanning Probe Microscopiesmentioning

confidence: 99%

“…[67] In the study already mentioned above where a laccase was immobilized on amorphous carbon nitride, correlation between AFM topography image and estimation of height profile via nanoscratching allowed detecting deformed enzymes standing flat on the surface. [76]…”

Section: Afmmentioning

confidence: 99%

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Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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Redox enzymes, which catalyze electron transfer reactions in living organisms, can be used as selective and sensitive bioreceptors in biosensors, or as efficient catalysts in biofuel cells. In these bioelectrochemical devices, the enzymes are immobilized at a conductive surface, the electrode, with which they must be able to exchange electrons. Different physicochemical methods have been coupled to electrochemistry to characterize the enzyme‐modified electrochemical interface. In this Review, we summarize most efforts performed to investigate the enzymatic electrodes at the micro‐ and even nanoscale, thanks to microscopy techniques. Contrary to electrochemistry, which gives only a global information about all processes occurring at the electrode surface, microscopy offers a spatial resolution. Several techniques have been implemented; mostly scanning probe microscopies like atomic force microscopy, scanning tunneling microscopy, and scanning electrochemical microscopy, but also scanning electron microscopy and fluorescence microscopy. These studies demonstrate that various information can be obtained thanks to microscopy at different scales. Electrode imaging has been performed to confirm the presence of enzymes, to quantify and localize the biomolecules, but also to evaluate the morphology of immobilized enzymes, their possible conformation changes upon turnover, and their orientation at the electrode surface. Local redox activity has also been imaged and kinetics has been resolved.

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Section: Morphology Of Immobilized Enzymesmentioning

confidence: 99%

Section: Enzyme Localizationmentioning

confidence: 99%

Section: Scanning Probe Microscopiesmentioning

confidence: 99%

Section: Afmmentioning

confidence: 99%

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Micro‐ and Nanoscopic Imaging of Enzymatic Electrodes: A Review

2019

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“…Functionalized AuNPs embedded in mesoporous carbon matrixes were shown to induce productive interactions between pyridine moiety on AuNPs and FeS site on formate dehydrogenase favoring direct formate/CO 2 conversion[44]. Porous graphite electrode was used as a platform for amorphous carbon nitride deposition (a-CNx)[45] presenting a versatile surface chemistry. Trametes versicolor Lac was bound to such electrode surface either through amide or imine coupling, and the two binding methods were combined.…”

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confidence: 99%

Recent advances in surface chemistry of electrodes to promote direct enzymatic bioelectrocatalysis

Mazurenko

Hitaishi

Lojou

2020

Current Opinion in Electrochemistry

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Redox enzymes catalyze major reactions in microorganisms to supply energy for life. Their use in electrochemical biodevices requires their integration on electrodes, while maintaining their activity and optimizing their stability. In return, such applicative development puts forward the knowledge on involved catalytic mechanisms, providing a direct electrode connection of the enzyme is fulfilled. Enzymes being large molecules with active site embedded in an insulating moiety, direct bioelectrocatalysis supposes strategies for specific orientation of the enzyme to be developed. In this review, we summarize recent advances during the past three years in the chemical modification of electrodes favoring direct electrocatalysis. We present the different methodologies used according to the electrode materials, including metals, carbon-based electrodes or porous structures, and discuss the gained insights into bioelectrocatalysis. We especially focus on enzyme engineering which appears as an emerging strategy for enzyme anchoring. Remaining challenges will be discussed with regards to these last findings.

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