Orientation Difference of Chemically Immobilized and Physically Adsorbed Biological Molecules on Polymers Detected at the Solid/Liquid Interfaces in Situ

Ye, Shuji; Nguyen, Khoi Tan; Boughton, Andrew P.; Mello, Charlene M.; Chen, Zhan

doi:10.1021/la903932w

Cited by 63 publications

(121 citation statements)

References 87 publications

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“…33,34 Our optimized peptide attachment chemistry described above results in surfaces with a helical axis of 37 ± 11°with respect to the surface (Figure 4, red). We interpret this result as approximately two-thirds of the surface-bound peptides connected by two triazoles as shown in Figure 2, while approximately one-third of the surface contains peptides that are bound by only one triazole linkage.…”

Section: ■ Characterizationmentioning

confidence: 99%

Preparation and Characterization of Biofunctionalized Inorganic Substrates

Dugger

Webb

2015

Langmuir

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Integrating the function of biological molecules into traditional inorganic materials and substrates couples biologically relevant function to synthetic devices and generates new materials and capabilities by combining biological and inorganic functions. At this so-called "bio/abio interface," basic biological functions such as ligand binding and catalysis can be co-opted to detect analytes with exceptional sensitivity or to generate useful molecules with chiral specificity under entirely benign reaction conditions. Proteins function in dynamic, complex, and crowded environments (the living cell) and are therefore appropriate for integrating into multistep, multiscale, multimaterial devices such as integrated circuits and heterogeneous catalysts. However, the goal of reproducing the highly specific activities of biomolecules in the perturbed chemical and electrostatic environment at an inorganic interface while maintaining their native conformations is challenging to achieve. Moreover, characterizing protein structure and function at a surface is often difficult, particularly if one wishes to compare the activity of the protein to that of the dilute, aqueous solution phase. Our laboratory has developed a general strategy to address this challenge by taking advantage of the structural and chemical properties of alkanethiol self-assembled monolayers (SAMs) on gold surfaces that are functionalized with covalently tethered peptides. These surface-bound peptides then act as the chemical recognition element for a target protein, generating a biomimetic surface in which protein orientation, structure, density, and function are controlled and variable. Herein we discuss current research and future directions related to generating a chemically tunable biofunctionalization strategy that has potential to successfully incorporate the highly specialized functions of proteins onto inorganic substrates.

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Section: ■ Characterizationmentioning

confidence: 99%

Preparation and Characterization of Biofunctionalized Inorganic Substrates

Dugger

Webb

2015

Langmuir

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“…Ye et al [170] made use of SFG with varying polarization of the light impinging onto the samples to quantitatively probe peptide orientations adsorbed on polymer surfaces coated on solid CaF 2 substrates. They used polystyrene (PS) and polystyrene maleimide (PS-MA) and the Cysteine-terminated cecropin P1 (CP1) as a probe peptide whose amide I and II bands were monitored.…”

Section: Probing Surface Functionalizationmentioning

confidence: 99%

Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Volpati

Aoki

Aléssio

et al. 2014

Advances in Colloid and Interface Science

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“…SFG is also used to study the formation of -sheet aggregates from human islet amyloid polypeptide (hIAPP) on membrane surfaces [175]. Besides membrane peptides and proteins, SFG has also been used to investigate physically adsorbed and chemically immobilized peptides and proteins at liquid/air [181] or solid/liquid interfaces [57,62,63,65,[189][190][191][192].…”

Section: Sfg: a Tool For The Biological Surface And Interfacementioning

confidence: 99%

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

Zhang

Han

et al. 2013

Chin. Sci. Bull.

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Research in biology and medicine is a rapidly expanding field incorporating some of the most fundamental questions concerning structure, function, and purpose. The forefront of new research demands access to advanced techniques and instrumentation capable of probing these unanswered questions. Over the past several decades, nano-scale materials and devices ranging from quasione dimensional quantum dots to two dimensional graphene sheets have been engineered and have found applications in nano-bio imaging and spectroscopy. In this review, the incorporation of nanomaterials into three influential spectroscopic and microscopic techniques including fluorescence microscopy, surface plasmon resonance, and sum frequency generation will be introduced. Fluorescence imaging has visualized nanomaterials as compliments or replacements to comparable organic fluorphores, act as a quencher for FRET-based sensing, and serve as a nanoscaffold for molecular beacons. Their versatility in coating materials makes nanomaterials an excellent targeting molecule for any cellular macromolecule or structure. In addition to the targeting capabilities of nanomaterials in fluorescence imaging, surface plasmon resonance has incorporated nanomaterials for applications in signal enhancement, selectivity of target molecules, and the development of more refined and accurate detection. Functionalized nanoparticles enhance the capabilities of sum frequency generation vibrational spectroscopy by providing unique surface chemistry which alters target molecule interactions and orientations. In summary, the incorporation of nanomaterials has greatly enhanced the field of biology and medicine and has allowed for the continual advancement of not only research but instrument development. nano-bio interfaces, optical spectroscopy, fluoroscence, SERS, SFG Citation:Zhang X X, Han X F, Wu F G, et al. Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors. Chin Sci Bull, 2013, 58: 25372556, doi: 10.1007/s11434-013-5700-y Nanomaterials, which size ranges (1-100 nm) coincide with fundamentally important length scales in physics, are considered to be promising building blocks for future electrical, optical and optoelectronic applications [1][2][3][4][5][6][7][8]. For example, in metals, the mean free path of an electron at room temperature is ~10-100 nm [9]; the Bohr radius of photo-excited electron-hole pairs in semiconductors is ~1-10 nm [10]; and the 1-100 nm dimension is the range over which molecules assemble into nucleic acids and proteins [11]. Nanostructures on this size scale provide a unique technology to determine many critical structure/function relationships of biological molecules at the cellular level without introducing substantial interference. Advances in nanotechnology have developed at several stages: materials, devices, and systems. A decade ago, extensive research has focused on the development of various methods to precisely control the synthesis of nanomaterials. Therefore, many differ...

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Orientation Difference of Chemically Immobilized and Physically Adsorbed Biological Molecules on Polymers Detected at the Solid/Liquid Interfaces in Situ

Cited by 63 publications

References 87 publications

Preparation and Characterization of Biofunctionalized Inorganic Substrates

Preparation and Characterization of Biofunctionalized Inorganic Substrates

Vibrational spectroscopy for probing molecular-level interactions in organic films mimicking biointerfaces

Nano-bio interfaces probed by advanced optical spectroscopy: From model system studies to optical biosensors

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