Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells

Gui, Zhifang; Li, Shuquan; Liu, Xing; Xu, Bin; Xu, Jian

doi:10.1186/s12906-015-0640-5

Cited by 21 publications

(16 citation statements)

References 42 publications

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“…The majority of them (19 DEMs) have been either verified to be associated with PC with the HMDD and miR2Disease databases or found to be differentially expressed in PC based on the Pancreatic Cancer Database (PCD) and TCGA. The rest DEMs were found to be differentially expressed in a recent study on human pancreatic cancer cells, especially hsa‐miR‐216b‐3p, hsa‐miR‐196b‐5p, hsa‐miR‐2114‐5p, and hsa‐miR‐216a‐3p whose expression level changes identified here were consistent with previous observations. This suggests that our results agree well with previous findings.…”

Section: Resultssupporting

confidence: 91%

“…DEMs were found to be differentially expressed in a recent study on human pancreatic cancer cells, 63 especially hsa-miR-216b-3p, hsa-miR-196b-5p, hsa-miR-2114-5p, and hsa-miR-216a-3p whose expression level changes identified here were consistent with previous observations. This suggests that our results agree well with previous findings.…”

Section: Analysis Of Differentially Expressed Mirnassupporting

confidence: 91%

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Network‐based integration of mRNA and miRNA profiles reveals new target genes involved in pancreatic cancer

Lin

Liang

et al. 2018

Molecular Carcinogenesis

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Pancreatic cancer is regarded as the most fatal and aggressive malignancy cancer due to its low 5‐year survival rate and poor prognosis. The approaches of early diagnosis and treatment are limited, which makes it urgent to identify the complex mechanism of pancreatic oncogenesis. In this study, we used RNA‐seq to investigate the transcriptomic (mRNA and miRNA) profiles of pancreatic cancer in paired tumor and normal pancreatic samples from ten patients. More than 1000 differentially expressed genes were identified, nearly half of which were also found to be differentially expressed in the majority of examined patients. Functional enrichment analysis revealed that these genes were significantly enriched in multicellular organismal and metabolic process, secretion, mineral transport, and intercellular communication. In addition, only 24 differentially expressed miRNAs were found, all of which have been reported to be associated with pancreatic cancer. Furthermore, an integrated miRNA‐mRNA interaction network was generated using multiple resources. Based on the calculation of disease correlation scores developed here, several genes present in the largest connected subnetwork, such as albumin, ATPase H+/K+ exchanging alpha polypeptide and carcinoembryonic antigen‐related cell adhesion molecule 1, were considered as novel genes that play important roles in the development of pancreatic cancer. Overall, our data provide new insights into further understanding of key molecular mechanisms underlying pancreatic tumorigenesis.

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Section: Resultssupporting

confidence: 91%

Section: Analysis Of Differentially Expressed Mirnassupporting

confidence: 91%

Network‐based integration of mRNA and miRNA profiles reveals new target genes involved in pancreatic cancer

Lin

Liang

et al. 2018

Molecular Carcinogenesis

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“…In our previous study, we found that oridonin alters the expression profiles of miRNAs in the BxPC-3 human pancreatic cancer cells, 105 miRNAs were significantly altered: 49 miRNAs were significantly downregulated, while 56 were significantly upregulated. Among them, miR-200b-3p was significantly downregulated (19), and also significantly downregulated in the PANC-1 cells (Fig. 1C).…”

Section: Discussionmentioning

confidence: 91%

“…A previous microarray study has shown that 105 miRNAs are differentially expressed in BxPC-3 cells treated with oridonin at concentrations of 0 and 87.8 µM (19). As shown in Fig.…”

Section: Oridonin Treatment Alters Mir-200b-3p Expression In Bxpc-3 Amentioning

confidence: 81%

Oridonin inhibition and miR-200b-3p/ZEB1 axis in human pancreatic cancer

Gui

Luo

Yang

et al. 2016

International Journal of Oncology

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The relationship among oridonin, miR-200b-3p and pancreatic cancer on epithelial-to-mesenchymal transition (EMT) was investigated for the molecular mechanism or signaling pathways on the migration in pancreatic cancer. BxPC-3 and PANC-1 cells were cultivated and the IC50 of oridonin in BxPC-3 and PANC-1 cells were obtained by the CCK-8 array. The expression of miR‑200b-3p was verified by using real-time PCR and its target gene was predicted. BxPC-3 and PANC-1 cells were treated with oridonin or transfected by miR-200b-3p, those cells were used for western blot assay, Transwell assay, ELISA, immunofluorescence staining, tumorigenesis assay in nude mice and immunohistochemical assay to verify the effects of oridonin or miR-200b-3p on pancreatic cancer. We found that oridonin inhibited the proliferation of BxPC-3 and PANC-1 cells in a dose-dependent manner. miR-200b-3p was downregulated by oridonin in BxPC-3 and PANC-1 cells. ZEB1 was a target gene for miR-200b-3p. Oridonin or overexpression of miR‑200b-3p can inhibit the cell migration in BxPC-3 and PANC-1 cells. miR-200b-3p can inhibit the EMT and oridonin can inhibit the expression of ZEB1, N-cadherin and fibronectin but not increase the expression of E-cadherin, while the cell adhesion molecules ICAM-1 and VCAM-1 were decreased by oridonin in BxPC-3 and PANC-1 cells and the cytoskeleton was altered by oridonin in PANC-1 cells compared with the control. In summary, the results demonstrate that miR‑200b-3p was able to inhibit the EMT of human pancreatic cancer in vivo and in vitro by targeted ZEB1. In vitro, oridonin had a certain effect on the migration in BxPC-3 and PANC-1 cells, but not though type III EMT by miR-200-3p/ZEB1 axis, and may be related to type Ⅱ EMT, tumor microenvironment or altering the cytoskeleton. In vivo, oridonin inhibited the cancer migration in the nude mouse model though inhibiting EMT.

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“…The diterpenes are multiedged swords in their activities. Dysoxydensin B, C and E isolated from Dysoxylum densiflorum exhibited a moderate cytotoxic effect on a number of human cancer cell lines (Gu et al , ), while oridonin is evident to inhibit the cell growth, probably via regulating the expression of miRNAs in BxPC‐3 human pancreatic cancer cells (Gui et al , ).…”

Section: Diterpenic Anticancer Traitsmentioning

confidence: 99%

Diterpenes and Their Derivatives as Potential Anticancer Agents

Islam

2017

Phytotherapy Research

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As therapeutic tools, diterpenes and their derivatives have gained much attention of the medicinal scientists nowadays. It is due to their pledging and important biological activities. This review congregates the anticancer diterpenes. For this, a search was made with selected keywords in PubMed, Science Direct, Web of Science, Scopus, The American Chemical Society and miscellaneous databases from January 2012 to January 2017 for the published articles. A total 28, 789 published articles were seen. Among them, 240 were included in this study. More than 250 important anticancer diterpenes and their derivatives were seen in the databases, acting in the different pathways. Some of them are already under clinical trials, while others are in the nonclinical and/or pre-clinical trials. In conclusion, diterpenes may be one of the lead molecules in the treatment of cancer. Copyright © 2017 John Wiley & Sons, Ltd.

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Oridonin alters the expression profiles of MicroRNAs in BxPC-3 human pancreatic cancer cells

Cited by 21 publications

References 42 publications

Network‐based integration of mRNA and miRNA profiles reveals new target genes involved in pancreatic cancer

Network‐based integration of mRNA and miRNA profiles reveals new target genes involved in pancreatic cancer

Oridonin inhibition and miR-200b-3p/ZEB1 axis in human pancreatic cancer

Diterpenes and Their Derivatives as Potential Anticancer Agents

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