Organospecific responses of lupin seedlings to lead Localization of hydrogen peroxide and peroxidase activity

Przymusiński, Roman; Ilska, Bogna; Gwóźdź, Edward A.

doi:10.1007/s11738-007-0049-y

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…H 2 O 2 is an example of ROS whose content increased in the presence of lead ions. A similar effect was found in many species of land plants, including Allium sativum [ 132 ], Brassica campestris [ 125 ], Lupinus luteus [ 133 ], Pisum sativum [ 102 ], Sedum alfredii [ 134 ], Talinum triangulare [ 135 ], Triticum aestivum [ 136 ], and Vicia faba [ 137 ]. There is little information about aquatic plants in this context.…”

Section: Discussionsupporting

confidence: 54%

Pb-Induced Avoidance-Like Chloroplast Movements in Fronds of Lemna trisulca L.

et al. 2015

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Lead ions are particularly dangerous to the photosynthetic apparatus, but little is known about the effects of trace metals, including Pb, on regulation of chloroplast redistribution. In this study a new effect of lead on chloroplast distribution patterns and movements was demonstrated in mesophyll cells of a small-sized aquatic angiosperm Lemna trisulca L. (star duckweed). An analysis of confocal microscopy images of L. trisulca fronds treated with lead (15 μM Pb2+, 24 h) in darkness or in weak white light revealed an enhanced accumulation of chloroplasts in the profile position along the anticlinal cell walls, in comparison to untreated plants. The rearrangement of chloroplasts in their response to lead ions in darkness was similar to the avoidance response of chloroplasts in plants treated with strong white light. Transmission electron microscopy X-ray microanalysis showed that intracellular chloroplast arrangement was independent of the location of Pb deposits, suggesting that lead causes redistribution of chloroplasts, which looks like a light-induced avoidance response, but is not a real avoidance response to the metal. Furthermore, a similar redistribution of chloroplasts in L. trisulca cells in darkness was observed also under the influence of exogenously applied hydrogen peroxide (H2O2). In addition, we detected an enhanced accumulation of endogenous H2O2 after treatment of plants with lead. Interestingly, H2O2-specific scavenger catalase partly abolished the Pb-induced chloroplast response. These results suggest that H2O2 can be involved in the avoidance-like movement of chloroplasts induced by lead. Analysis of photometric measurements revealed also strong inhibition (but not complete) of blue-light-induced chloroplast movements by lead. This inhibition may result from disturbances in the actin cytoskeleton, as we observed fragmentation and disappearance of actin filaments around chloroplasts. Results of this study show that the mechanisms of the toxic effect of lead on chloroplasts can include disturbances in their movement and distribution pattern.

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Section: Discussionsupporting

confidence: 54%

Pb-Induced Avoidance-Like Chloroplast Movements in Fronds of Lemna trisulca L.

et al. 2015

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“…The excess of TMs in plant cells can cause a range of toxic alterations, e.g., the generation of reactive oxygen species (ROS) resulting in oxidative stress (Rucińska-Sobkowiak and Pukacki 2006;Przymusiński et al 2007), which leads to the destruction of cell membranes: the PM, the endomembrane system and membranous organelles, in particular chloroplast, mitochondrion (Hall 2002;Patra et al 2004) and nucleus (Prasad 2004;Glińska et al 2007). Furthermore, trace metals, especially non-essential ones, can destroy enzyme functioning by entering in place of proper ions for building their molecules or by binding to their sulfhydryl and carboxyl groups, thereby changing their conformation (Hall 2002;Patra et al 2004).…”

Section: Introductionmentioning

confidence: 99%

The cell wall in plant cell response to trace metals: polysaccharide remodeling and its role in defense strategy

2010

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This review paper is focused predominantly on the role of the cell wall in the defense response of plants to trace metals. It is generally known that this compartment accumulates toxic divalent and trivalent metal cations both during their uptake by the cell from the environment and at the final stage of their sequestration from the protoplast. However, from results obtained in recent years, our understanding of the role played by the cell wall in plant defense response to toxic metals has markedly altered. It has been shown that this compartment may function not only as a sink for toxic trace metal accumulation, but that it is also actively modified under trace metal stress. These modifications lead to an increase in the capacity of the cell wall to accumulate trace metals and a decrease of its permeability for trace metal migration into the protoplast. One of the most striking alterations is the enhancement of the level of low-methylesterified pectins: the polysaccharides able to bind divalent and trivalent metal ions. This review paper will present the most recent results, especially those that are concerned with polysaccharide level, composition and distribution under trace metal stress, and describe in detail the polysaccharides responsible for metal binding and immobilization in different groups of plants (algae and higher plants). The review also contains information related to the entry pathways of trace metals into the cell wall and their detection methods.

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“…This result complements the previous studies carried out on lupine roots, where an increased level of free radicals (Rucińska et al 1999) and superoxide dismutase activity (Przymusiński et al 1995) was demonstrated in lead treated lupine seedlings. It has been proved that H 2 O 2 induces gene expression of PR-proteins and their accumulation under lead treatment (Przymusiński et al 2001(Przymusiński et al , 2007.…”

Section: Resultsmentioning

confidence: 99%