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Key words: soy bean, cad mium, ni tric ox ide, superoxide dismutase, ox i dized stress, con fo cal mi cros copy, ox i dized pro teins, re ac tive ox y gen spe cies, so dium nitroprusside, cell sus pen sion Ab stractNi tric ox ide (NO) is a small, ubiq ui tous mol e cule, whose physi o log i cal func tion in plants has re cently been widely in ves tigated. It seems that one of its piv otal prop er ties is the an ti ox idant ca pac ity, en abling plants to al le vi ate the ef fects of the ox idized stress. In this work we in ves ti gated the role of NO in soybean (Glycine max L. Cv. Navico) cell sus pen sion treated with cad mium. So dium nitroprusside (SNP), ni tric ox ide do nor, mark edly de creased the neg a tive in flu ence of Cd 2+ on cell growth. It was also found to stim u late superoxide dismutase (SOD, EC 1.15.1.1). Us ing spe cific fluorochromes -dihydroethidine (DHE) and 2',7'-dichlorofluorescein (DCFH-DA) it was shown that NO was very ef fec tive in re ducing the level of superoxide an ion (O 2 ·-) and hy dro gen per ox -ACTA PHYSIOLOGIAE