Organoselenium Coating on Cellulose Inhibits the Formation of Biofilms byPseudomonas aeruginosaandStaphylococcus aureus

Abstract

Among the most difficult bacterial infections encountered in treating patients are wound infections, which may occur in burn victims, patients with traumatic wounds, necrotic lesions in people with diabetes, and patients with surgical wounds. Within a wound, infecting bacteria frequently develop biofilms. Many current wound dressings are impregnated with antimicrobial agents, such as silver or antibiotics. Diffusion of the agent(s) from the dressing may damage or destroy nearby healthy tissue as well as compromise the effectiveness of the dressing. In contrast, the antimicrobial agent selenium can be covalently attached to the surfaces of a dressing, prolonging its effectiveness. We examined the effectiveness of an organoselenium coating on cellulose discs in inhibiting Pseudomonas aeruginosa and Staphylococcus aureus biofilm formation. Colony biofilm assays revealed that cellulose discs coated with organoselenium completely inhibited P. aeruginosa and S. aureus biofilm formation. Scanning electron microscopy of the cellulose discs confirmed these results. Additionally, the coating on the cellulose discs was stable and effective after a week of incubation in phosphatebuffered saline. These results demonstrate that 0.2% selenium in a coating on cellulose discs effectively inhibits bacterial attachment and biofilm formation and that, unlike other antimicrobial agents, longer periods of exposure to an aqueous environment do not compromise the effectiveness of the coating.

Biogenic selenium nanoparticles synthesized by Stenotrophomonas maltophilia SeITE02 loose antibacterial and antibiofilm efficacy as a result of the progressive alteration of their organic coating layer

“…In particular, antimicrobial effects of different selenium compounds have been attributed to the formation of free radicals (Tran et al ., 2009). In this study, exposure of three bacterial strains (namely P .…”

Organo-Selenium-containing Dental Sealant Inhibits Bacterial Biofilm

“…Also, we have shown, in a previous study, that organo-selenium in polymer form can generate superoxide radicals (Tran et al, 2009).…”

“…The superoxide radical appears to account for most of selenium's toxicity toward different bacteria, such as Staphylococcus epidermidis, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, and Escherichia coli in vitro (Babior et al, 1975;Bortolussi et al, 1987;Kramer and Ames, 1988). We previously showed that organo-selenium covalently attached to different biomaterials and medical devices, such as intravenous catheter, contact lenses, and reverse osmosis (RO) membranes, as well as cellulose, blocks the formation of Staphylococcus aureus and Pseudomonas aeruginosa biofilms (Mathews et al, 2006;Tran et al, 2009Tran et al, , 2012Reid et al, 2010;Low et al, 2011). Low concentrations of organo-selenium (0.1% or 0.2%) were sufficient to inhibit bacterial attachment to these materials.…”

Selenium nanoparticles inhibit Staphylococcus aureus growth

“…15,16 Others reported inhibitory effects of selenium compounds (in the form of coatings) on the growth of some types of bacteria. 17 However, the influence of selenium nanoparticles on bacteria growth remains largely unexplored. …”