Organophosphorus Pesticides Determination by Electrochemical Biosensors

Stoytcheva, Margarita; Zlatev, Roumen; Velkova, Zdravka; Valdez, Benjamín

doi:10.5772/13690

Cited by 7 publications

(6 citation statements)

References 64 publications

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“…The most extensively reported enzymes were acetylcholinesterase (AChE) and butyryl-cholinesterase (BChE), which were widely regarded as the initial enzymatic sensors for the detection of organophosphates. The enzyme-catalyzed hydrolysis of the choline esters results in a decrease in the amount of acid released, and hence, causing a shift in the pH, which was further detected by the AchE biosensors [ 62 ]. The amperometric AChE biosensors function via detecting the concentration of thiocholine, a hydrolysate of acetylthiocholine.…”

Section: Expanded Application Of the Pollutant-degrading Enzymes In Industriesmentioning

confidence: 99%

Pollutant Degrading Enzyme: Catalytic Mechanisms and Their Expanded Applications

Zhang

et al. 2021

Molecules

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The treatment of environmental pollution by microorganisms and their enzymes is an innovative and socially acceptable alternative to traditional remediation approaches. Microbial biodegradation is often characterized with high efficiency as this process is catalyzed via degrading enzymes. Various naturally isolated microorganisms were demonstrated to have considerable ability to mitigate many environmental pollutants without external intervention. However, only a small fraction of these strains are studied in detail to reveal the mechanisms at the enzyme level, which strictly limited the enhancement of the degradation efficiency. Accordingly, this review will comprehensively summarize the function of various degrading enzymes with an emphasis on catalytic mechanisms. We also inspect the expanded applications of these pollutant-degrading enzymes in industrial processes. An in-depth understanding of the catalytic mechanism of enzymes will be beneficial for exploring and exploiting more degrading enzyme resources and thus ameliorate concerns associated with the ineffective biodegradation of recalcitrant and xenobiotic contaminants with the help of gene-editing technology and synthetic biology.

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Section: Expanded Application Of the Pollutant-degrading Enzymes In Industriesmentioning

confidence: 99%

Pollutant Degrading Enzyme: Catalytic Mechanisms and Their Expanded Applications

Zhang

et al. 2021

Molecules

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“…It is well known that organophosphorus and carbamate pesticides quantitatively inhibit cholinesterase. For this reason, amperometric biosensors based on inhibition of AChE have been extensively applied to rapid, simple, and selective analysis of pesticides (organophosphorous and carbamates) [11,12].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensor Based on Optimized Biocomposite for Organophosphorus and Carbamates Pesticides Detection

Montes

Céspedes

Gabriel

et al. 2018

Journal of Nanomaterials

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This paper presents the characterization and optimization of biosensors based on graphite-epoxy which incorporates the enzyme acetylcholinesterase (AChE). By means of advanced electrochemical techniques, such as electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV), the characterization and optimization of graphite-epoxy-AChE biosensors have been performed. In order to obtain sensitive electrodes, the optimal composition of the transducer material (graphite-epoxy-enzyme ratio) was studied. The optimization of the conductive particles distribution inside the biomaterial has allowed an improvement of the electrochemical properties. Optimal composition guarantees improving electrochemical properties required, such as high electron-transfer rate, high signal-to-noise ratio, and suitable sensitivity. The optimal biocomposite composition range was obtained between 16% and 17% of graphite and 0.12% of AChE. The biosensors were applied to the analysis of different pesticides, organophosphorus and carbamates, using indirect measurements based on enzymatic inhibition process. These optimized biosensors present detection limit one order of magnitude lower compared to the standard composition (nonoptimized) and allow achieving concentrations lower than the established ones by the pesticides regulation. Finally, spiked tap water samples with pesticides were analyzed with the optimized biosensors.

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“…These risks call for the development of sensitive and field-deployable screening methods of low cost for their rapid determination, as an alternative to the timeconsuming and expensive chromatographic laboratory techniques, currently in use [8][9][10]. As reported in the literature, the electrochemical methods are well suited for such analytical purposes [8,[11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…The electrochemical methods of choice for organophosphorus pesticides determination are the biosensors based ones [8,12,14,15]. They take advantage of the cholinesterases activity inhibition by OPs or of the ability of the organophosphorus hydrolase (OPH) to catalyse their hydrolysis.…”

Section: Introductionmentioning

confidence: 99%

A nanotechnological approach to biosensors sensitivity improvement: application to organophosphorus pesticides determination

Stoytcheva

Zlatev

Montero

et al. 2017

Biotechnology & Biotechnological Equipment

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An electrochemical organophosphorus hydrolase-based sensor for direct organophosphorus pesticides determination was developed, analytically characterized and applied for paraoxon quantification. The biosensing platform was constructed by one-step electrodeposition onto the surface of a glassy carbon electrode of a bionanocomposite of chitosan with carbon nanotube (CNTs), hydroxyapatite (HA) and organophosphorus hydrolase. The sensor, optimized with respect to the CNTs and HA load, was characterized by the application of cyclic voltammetry and chronoamperometry. The limit of detection (LOD) was found to be as low as 0.1 mmol L ¡1 , the linear concentration range was extended up to 80 mmol L ¡1 , and the sensitivity was as high as 5.10 nA L mmol ¡1 . The sensor possesses enhanced sensitivity towards organophosphorus pesticides (OPs), because of the CNTs and the HA nanoparticles synergistic action.

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Organophosphorus Pesticides Determination by Electrochemical Biosensors

Cited by 7 publications

References 64 publications

Pollutant Degrading Enzyme: Catalytic Mechanisms and Their Expanded Applications

Pollutant Degrading Enzyme: Catalytic Mechanisms and Their Expanded Applications

Electrochemical Biosensor Based on Optimized Biocomposite for Organophosphorus and Carbamates Pesticides Detection

A nanotechnological approach to biosensors sensitivity improvement: application to organophosphorus pesticides determination

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