Organoid Studies in COVID-19 Research

Kim, Jihoon; Koo, Bon‐Kyoung

doi:10.15283/ijsc21251

Cited by 18 publications

(9 citation statements)

References 72 publications

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“…Aside from mechanistic findings, it has been proposed by Bose [89] that lung organoids originating from varying ethnicities can be used to understand the infectivity of SARS-CoV-2 across the world. Further findings using organoids are also documented in numerous reviews [53,88,[90][91][92][93].…”

Section: Respiratory Organoidmentioning

confidence: 75%

3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases

2022

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The COVID-19 pandemic has driven the scientific community to adopt an efficient and reliable model that could keep up with the infectious disease arms race. Coinciding with the pandemic, three dimensional (3D) human organoids technology has also gained traction in the field of infectious disease. An in vitro construct that can closely resemble the in vivo organ, organoid technology could bridge the gap between the traditional two-dimensional (2D) cell culture and animal models. By harnessing the multi-lineage characteristic of the organoid that allows for the recapitulation of the organotypic structure and functions, 3D human organoids have emerged as an essential tool in the field of infectious disease research. In this review, we will be providing a comparison between conventional systems and organoid models. We will also be highlighting how organoids played a role in modelling common infectious diseases and molecular mechanisms behind the pathogenesis of causative agents. Additionally, we present the limitations associated with the current organoid models and innovative strategies that could resolve these shortcomings.

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Section: Respiratory Organoidmentioning

confidence: 75%

3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases

2022

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“…Although hPSC-AECs offer a robust in vitro tool to assess pulmotoxicity of various air pollutants and harmful chemicals, the 2D AEC cultures remain incomplete as they lack of key components of alveolar tissues, including immune cells and vessels. Moreover, recent progress in 3D organoid system has gained great attention for the replacement of 2D culture system ( Lee et al., 2021 ; Heo et al, 2022 ; Kim et al, 2022 ; Kook et al, 2022 ; Kwon et al., 2022 ). Thus, development of 3D alveolar organoid system with integration of key missing components will facilitate a significant advancement in the in vitro toxicity screening platform.…”

Section: Resultsmentioning

confidence: 99%

Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract

Kim¹,

Kang²,

Jung³

et al. 2022

Dev. Reprod.

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Human pluripotent stem cells (hPSCs) can give rise to a vast array of differentiated derivatives, which have gained great attention in the field of in vitro toxicity evaluation. We have previously demonstrated that hPSC-derived alveolar epithelial cells (AECs) are phenotypically and functionally similar to primary AECs and could be more biologically relevant alternatives for assessing the potential toxic materials including in fine dust and cigarette smoking. Therefore, in this study, we employed hPSC-AECs to evaluate their responses to exposure of various concentrations of diesel particulate matter (dPM), cigarette smoke extract (CSE) and nicotine for 48 hrs in terms of cell death, inflammation, and oxidative stress. We found that all of these toxic materials significantly upregulated the transcription of pro-inflammatory cytokines such as IL-1α , IL-β , IL-6 , and TNF-α . Furthermore, the exposure of dPM (100 μg/mL) strongly induced upregulation of genes related with cell death, inflammation, and oxidative stress compared with other concentrations of CSE and nicotine. These results suggest that hPSC-AECs could be a robust in vitro platform to evaluate pulmotoxicity of various air pollutants and harmful chemicals.

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“…While research on infectious pulmonary diseases has been conducted before, the outbreak of the COVID-19 pandemic in 2019 urged a rapid increase in the research on in vitro respiratory models for investigating viral infections ( Table 1 ). 155 …”

Section: Respiratory Infection Modelingmentioning

confidence: 99%

Advanced lung organoids for respiratory system and pulmonary disease modeling

Joo,

Min,

Cho

2024

J Tissue Eng

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Amidst the recent coronavirus disease 2019 (COVID-19) pandemic, respiratory system research has made remarkable progress, particularly focusing on infectious diseases. Lung organoid, a miniaturized structure recapitulating lung tissue, has gained global attention because of its advantages over other conventional models such as two-dimensional (2D) cell models and animal models. Nevertheless, lung organoids still face limitations concerning heterogeneity, complexity, and maturity compared to the native lung tissue. To address these limitations, researchers have employed co-culture methods with various cell types including endothelial cells, mesenchymal cells, and immune cells, and incorporated bioengineering platforms such as air-liquid interfaces, microfluidic chips, and functional hydrogels. These advancements have facilitated applications of lung organoids to studies of pulmonary diseases, providing insights into disease mechanisms and potential treatments. This review introduces recent progress in the production methods of lung organoids, strategies for improving maturity, functionality, and complexity of organoids, and their application in disease modeling, including respiratory infection and pulmonary fibrosis.

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Organoid Studies in COVID-19 Research

Cited by 18 publications

References 72 publications

3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases

3D Human Organoids: The Next “Viral” Model for the Molecular Basis of Infectious Diseases

Human Pluripotent Stem Cell-Derived Alveolar Epithelial Cells as a Tool to Assess Cytotoxicity of Particulate Matter and Cigarette Smoke Extract

Advanced lung organoids for respiratory system and pulmonary disease modeling

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