Organogenesis and somatic embryogenesis in callus cultures ofRosa hybrida andRosa chinensis minima

Hsia, Chi Ni; Korban, Schuyler S.

doi:10.1007/bf00045906

Cited by 65 publications

(33 citation statements)

References 13 publications

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“…Similar results were found on media containing sucrose and fructose. The superiority of glucose in promoting shoot regeneration from stem explants of P. amurense is consistent with the results obtained with Beech, Rosa hybrid and chinensis minima (Cuenca and Vieitez, 2000;Hsia and Korban, 1996;Lemos and Baker, 1998). This may be because that glucose is more efficiently uptake and transported in tissues or more immediately used to provide energy for shoot organogenesis.…”

Section: Effect Of Carbon Source On Shoot Regenerationsupporting

confidence: 89%

Adventitious shoot regeneration from in vitro stem explants of Phellodendron amurense

Mei¹,

Jie²,

Di³

et al. 2011

Afr. J. Biotechnol.

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An efficient in vitro plant regeneration system from stem explants was established in Phellodendron amurense. Factors influencing shoot regeneration from stems including culture medium type, combinations of plant growth regulators and carbon source in the medium were investigated. Adventitious shoot regeneration was significantly influenced by the type of medium. Murashige and Skoog medium (MS) was the best for promoting shoot regeneration, followed by Gamborg medium (

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Section: Effect Of Carbon Source On Shoot Regenerationsupporting

confidence: 89%

Adventitious shoot regeneration from in vitro stem explants of Phellodendron amurense

Mei¹,

Jie²,

Di³

et al. 2011

Afr. J. Biotechnol.

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“…In general, sucrose is commonly used in tissue culture media to induce adventitious shoots or buds. However, there are few reports whereby glucose has been found to be better source of carbon than sucrose (Hsia and Korban 1996, Sul and Korban 1998, Cuenca and Vietiez 2000. Among carbon sources tested, sucrose was found best for multiple shoot proliferation of banana.…”

Section: ⎯⎯⎯⎯mentioning

confidence: 98%

Effects of carbon sources and auxins on in vitro propagation of banana

Madhulatha¹,

Kirubakaran²,

Sakthivel³

2006

Biol. plant.

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The effects of carbon sources (sucrose, glucose, fructose and mannitol) and auxins [indolebutyric acid (IBA) and α-naphthaleneacetic acid (NAA)] on in vitro propagation of banana (Musa spp. AAA) were studied. Over all carbon sources tested, sucrose induced highest frequency of shoot proliferation. Optimal shoot proliferation rates were achieved on the Murashige and Skoog (MS) medium supplemented with sucrose and glucose combination (1:1) at the concentration of 30 g dm -3 . Similarly, higher frequency of root induction was obtained at IBA and NAA combination (1:1; concentration of 2 mg dm -3 ) than at other concentrations of IBA or NAA alone or their combinations.

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“…These researchers observed also that the tissue producing roots diff erentiated shoots very rarely. On the other hand, in some plant species shoots regenerated only from callus with the high level of rhizogenesis (Hsia and Korban, 1996). Because auxin is gradually degraded on the light (Stasinopoulos and Hangarter, 1990), hence may arise a much higher degree of the rhizogenesis noted for the explants cultivated in the dark.…”

Section: Discussionmentioning

confidence: 99%

Callus Induction and Rhizogenesis in Lathyrus sativus L.

Piwowarczyk¹,

Pindel²,

Muszyńska³

2016

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques, including in vitro culture, are new and modern tools supporting plant breeding. Unfortunately, grass pea belongs to grain legumes that are well-known for their recalcitrance to in vitro manipulation and consequently plant regeneration. The aim of the study was the evaluation of diff erent factors infl uence on callus induction and proliferation, as well as cell diff erentiation and organogenesis. Callus culture were initiated from diff erent explants of two Polish cultivars of grasspea on media supplemented with auxin and various cytokinins both on the light and in the dark. The rate of tissue proliferation was signifi cantly increased in both tested cultivars by light. More than 400 mg of tissue from one explant was obtained a er 12 weeks of culture. The most intensive increase in callus mass was noted for internode fragments of 'Krab' (380 mg/one explant) and root fragments of 'Derek' (361 mg/one explant). On the media with the addition of thidiazuron callus tissue grew better (852 mg/one explants) than on the media with zeatin and kinetin (56-164 mg/one explant). Formation of the roots was the only type of organogenesis observed during the study. In 'Derek' callus on LP medium rhizogenesis occurred the most frequently (38%). The roots regenerated from this callus was also the most numerous (1.6) and the longest (12.5 mm).

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Organogenesis and somatic embryogenesis in callus cultures ofRosa hybrida andRosa chinensis minima

Cited by 65 publications

References 13 publications

Adventitious shoot regeneration from in vitro stem explants of Phellodendron amurense

Adventitious shoot regeneration from in vitro stem explants of Phellodendron amurense

Effects of carbon sources and auxins on in vitro propagation of banana

Callus Induction and Rhizogenesis in Lathyrus sativus L.

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