Effects of carbon sources and auxins on in vitro propagation of banana

Abstract

The effects of carbon sources (sucrose, glucose, fructose and mannitol) and auxins [indolebutyric acid (IBA) and α-naphthaleneacetic acid (NAA)] on in vitro propagation of banana (Musa spp. AAA) were studied. Over all carbon sources tested, sucrose induced highest frequency of shoot proliferation. Optimal shoot proliferation rates were achieved on the Murashige and Skoog (MS) medium supplemented with sucrose and glucose combination (1:1) at the concentration of 30 g dm -3 . Similarly, higher frequency of root induction was obtained at IBA and NAA combination (1:1; concentration of 2 mg dm -3 ) than at other concentrations of IBA or NAA alone or their combinations.

Musa sp. paradisiaca Cultivar Gopi and Clonal fidelity Assessment using ISSR Marker

“…1E). Necessity of auxin application for better rooting of banana cultivars has been reported in previous works (Madhulata et al 2006, Safarpour et al 2017. Hardening of the in vitro regenerants in field conditions was successful with high survival rate and they were also healthy with no morphological abnormalities (Fig.…”

Abscisic acid and auxin accumulation in Catasetum fimbriatum roots growing in vitro with high sucrose and mannitol content

“…However, as the increase in root length at 4 % sucrose, compared to the standard 2 % condition for orchids (Vacin andWent 1949, Wotavová-Novotná et al 2007), could also be attributed to nutritional effects, we tested various combinations of 2 % sucrose plus mannitol as an osmoticum. As in other species (Stoop et al 1996, Madhulatha et al 2006, it seems that mannitol is not metabolized by C. fimbriatum roots, since there is no significant difference between root length at 0 % sucrose and 1.08 % mannitol (Table 1). Treatments with mannitol also presented a significant decrease in root water content, combined with increased Table 1.…”

Micropropagation and Assessment of Genetic Stability of Musa sp. cv. Williams using RAPD and SRAP Markers.

“…In our study, we providea very simple efficient protocol for commercial banana micropropagation using MS medium supplemented with 3mg/l BAP and 0.2mg/l NAA, followed by 3mg/l IAA for rooting, which induced a vigorous root system. Similarly, previous reports have declared the necessity of using auxins for better rooting (Madhulatha et al, 2006 andSafarpour et al, 2017). According to Gebeyehu (2015) banana plantlets regenerated through tissue culture have higher survival rate and reduce the cost of disease management.…”