Organogénesis a partir de hojas de lisianthus (Eustoma grandiflorum) en respuesta a diferentes reguladores de crecimiento

Abstract

Lisianthus´ characteristics make it a cut flower of great interest, so the use of micropropagation techniques favors a rapid and massive multiplication, with plants free of pathogens. The organogenesis, resulted of use regulators of growth of different action mode (auxin, cytokinin and gibberellin), included the formation of shoots and roots that it was generated in some cases directly and in other from callus, employing the foliar segments as explant. The addition of low concentrations of cytokinin and gibberellin (1.0 mg/L benzyladenine (BA) + 0.5 mg/L gibberellic acid (GA3)) to the culture medium were necessary to generate the highest number of shoots (14.16 shoots per explant).

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“…It was observed that the treatment with MS I medium and intermediate explant showed the highest mean value of multiplication, with 3.625 shoots per explant (Table 2). The higher ratio of cytokinin/auxin in the MS I medium, together with the use of the intermediate explant in which apical dominance is interrupted, are some of the factors that explain this superior response compared to the other treatments (Kaviani, 2015;Falcón-Bautista et al, 2018). The results of this study refer to the first subculture of the multiplication phase.…”

“…These results may be explained by the fact that the MS II culture medium has a lower cytokinin/auxin ratio compared to the MS I medium . The lower ratio favors the direction of explant metabolism towards elongation rather than multiplication (Oliveira et al, 2011, Falcón-Bautista et al, 2018. For the trait of number of shoots emitted per explant, the treatments did not differ statistically, as shown by ANOVA (p > 0.05).…”

Effect of growth regulators BAP and NAA for in vitro multiplication and elongation of Corymbia citriodora juvenile explants

“…It was observed that the treatment with MS I medium and intermediate explant showed the highest mean value of multiplication, with 3.625 shoots per explant (Table 2). The higher ratio of cytokinin/auxin in the MS I medium, together with the use of the intermediate explant in which apical dominance is interrupted, are some of the factors that explain this superior response compared to the other treatments (Kaviani, 2015;Falcón-Bautista et al, 2018). The results of this study refer to the first subculture of the multiplication phase.…”

“…These results may be explained by the fact that the MS II culture medium has a lower cytokinin/auxin ratio compared to the MS I medium (Xavier et al, 2013). The lower ratio favors the direction of explant metabolism towards elongation rather than multiplication (Oliveira et al, 2011, Falcón-Bautista et al, 2018. For the trait of number of shoots emitted per explant, the treatments did not differ statistically, as shown by ANOVA (p > 0.05).…”