Effect of growth regulators BAP and NAA for in vitro multiplication and elongation of Corymbia citriodora juvenile explants

Abstract

Corymbia citriodora is a species of great importance to Brazilian forestry. Vegetative propagation of selected Corymbia genotypes has been a challenge due to low rooting percentages. Micropropagation represents an alternative to advance the cloning techniques. This study aimed to evaluate the use of growth regulators BAP and NAA in vitro elongation and multiplication of Corymbia explants. A completely randomized experimental design was adopted, with six treatments defined by two culture medium compositions and three types of explants (MS I + base; MS I + intermediate; MS I + apical and MS II + base; MS II + intermediate; MS II + apical),with eight replications (tubes) with one explant per replication. The statistical analysis comprises of a residual normality test (Shapiro-Wilk), followed by an analysis of variance (ANOVA) using the F-test. We did not find a significant difference between treatments for the multiplication and elongation of Corymbia citriodora.