Organochlorine Pesticides in Specimens from Women Undergoing Spontaneous Abortion, Premature or Full-Term Delivery

Saxena, M.; Siddiqui, M.K.J.; Seth, T. D.; Murti, C. R. Krishna; Bhargava, A. K.; Kutty, D

doi:10.1093/jat/5.1.6

Cited by 107 publications

(51 citation statements)

References 8 publications

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“…In mammals, some studies have described relationships between organochlorine pesticides and preterm birth (Longnecker et al 2001;Saxena et al 1981). DDT metabolites are able to impede the binding of some sex steroids (androgen or progesterone) to their receptor through indirect or direct paths, shortening the duration of gestation (Klotz et al 1997;Lyon and Glenister 1980).…”

Section: Data)mentioning

confidence: 99%

The stress of being contaminated? Adrenocortical function and reproduction in relation to persistent organic pollutants in female black legged kittiwakes

Tartu

Angelier

Herzke

et al. 2014

Science of The Total Environment

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High levels of environmental pollutants such as persistent organic pollutants (POPs) including PCB and DDT have been found in the Arctic and many of those pollutants may impair reproduction through endocrine disruption. Nevertheless, their effects on stress hormones remain poorly understood, especially in free-ranging birds. Corticosterone, the principal glucocorticoid in birds, can indirectly impair reproduction. The aim of the present study was to examine the relationships between POPs and reproduction through their potential consequences on different reproductive traits (breeding decision, egg-laying date, breeding success) and corticosterone secretion (baseline and stress-induced levels). We addressed those questions in an Arctic population of female black-legged kittiwakes during the pre-breeding stage and measured several legacy POPs (PCBs and pesticides: HCB, p,p'-DDE, CHL) in whole blood. POP levels were not related to breeding decision neither to breeding success, whereas females with high levels of pesticides laid their eggs earlier in the season. We found a negative relationship between POP levels and body condition index in non-breeding females. Black-legged kittiwakes with higher levels of PCB showed stronger adrenocortical response when subjected to a capture-handling stress protocol.We suggest that PCBs may disrupt corticosterone secretion whereas the positive relationship between pesticides and egglaying date could either originate from a direct effect of pesticides or may be related to other confounding factors such as age or individual's quality. Although no direct negative reproduction output of POPs were found in this study, it is possible that the most contaminated individuals would be more sensitive to environmental stress and would be less able to maintain parental investment than less polluted individuals.

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Section: Data)mentioning

confidence: 99%

The stress of being contaminated? Adrenocortical function and reproduction in relation to persistent organic pollutants in female black legged kittiwakes

Tartu

Angelier

Herzke

et al. 2014

Science of The Total Environment

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“…Recent reports suggested an association between exposure to pesticides and different types of human cancer (Amaral Mendes, 2002;Safi, 2002). The facts that such residues persist in adipose tissue over a period of many decades, are endocrine disrupters, cause immune suppression, and inhibit various enzymes, lead to higher susceptibility to cancer onset (Iscan et al, 2002;Wolff et al, 2000;Safi, 2002;Saxena et al, 1981). Biological monitoring of exposure can be carried out by determination of intact compounds or their metabolites in blood, serum, plasma and urine (Aprea et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of organochlorine pesticide residues in human serum from an urban and two rural populations in Portugal

Cruz

Lino

Silveira

2003

Science of The Total Environment

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Organochlorine pesticide residues were measured in human serum from an urban and two rural populations in Portugal, in an attempt to evaluate the contamination level of Portuguese population. Serum levels of 12 residues were determined using a validated methodology that included gas chromatography-electron-capture detection. It was made an attempt to point out the differences of contamination between rural and urban populations; and among these, if it could be established a relation with sex and with age of individuals. p,p9DDE, a-hexachlorocyclohexane (HCH), p,p9DDD, and b-HCH were the most frequently identified residues. p,p9DDE concentrations ranged from undetected to 390.5 mgyl in urban samples, and from undetected to 43.5 mgyl and to 171.2 mgyl in both rural samples. Maximum a-HCH concentration level was 114.4 mgyl in urban samples, 261.3 and 45.5 mgyl in both rural samples. Mean total DDT levels were always higher than mean total HCH levels. About p,p9DDE, in all three populations, the majority of the results above the limit of quantification were found among female sex. The analysis of different aged groups showed that younger groups continue to reveal contamination. Comparing obtained results with others from Europe, Asia and America, it was observed that Portugal is between the highest levels of contamination. ᮊ

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“…However, in vitro exposure of rat uterine strips isolated from pregnant (Gestation Day 10) rats to cumulative concentrations of lindane at 10-min intervals was found to depress spontaneous contractions at 10 μM lindane and abolish contractions at 30 μM lindane (Criswell and Loch-Caruso, 1999). The inhibitory action of lindane was in contrast to stimulation of uterine contraction frequency that we observed with DDT (Juberg and Loch-Caruso, 1991;Juberg et al, , 1995 and polychlorinated biphenyl isomers (Tsai et al, 1996;Bae et al, 1999a Bae et al, ,b, 2001, which were also elevated in the blood of women who experienced premature delivery or spontaneous abortion (Saxena et al, 1981;Wassermann et al, 1982). We subsequently used lindane as an inhibitor for probing physiologic and pathologic mechanisms of regulation of uterine contraction, and identified a previously unrecognized role for reactive oxygen species (Krieger and Loch-Caruso, 2001) and a previously unidentified myometrial phospholipase A 2 activity (Wang et al, 2001).…”

Section: Introductionmentioning

confidence: 63%

“…In preliminary experiments we observed that uterine strips excised from nearterm (Gestation Day 20) pregnant rats exhibited depressed spontaneous, carbachol-induced, and oxytocin-induced contractions 24 h after a single subcutaneous injection of 4 or 6 mg lindane/kg body weight (unpublished). In women admitted to a hospital in India for obstetrical care who subsequently experienced preterm birth or spontaneous abortion, blood levels of lindane averaged 39.7 ppb (15-79 ppb range, n = 15) and 73.3 ppb (40-136 ppb range, n = 10), respectively (Saxena et al, , 1981. In another study of 17 Israeli women, significantly elevated concentrations of lindane (15 ppb) were detected in serum lipids of women who delivered prematurely compared with women who delivered at term (4.3 ppb) (Wassermann et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

“…The present study utilizes lindane, the γ-isomer of hexachlorocyclohexane, to investigate mechanisms of inhibition of spontaneous uterine contractions. Our laboratory initially investigated lindane's actions on uterine contraction because it was one of several organochlorine substances found in significantly elevated concentrations (15-136 ppb) in the blood lipids of women who had experienced spontaneous abortions or premature delivery compared with women who had undergone full-term pregnancy (7-39 ppb) (Saxena et al, 1981;Wassermann et al, 1982). However, in vitro exposure of rat uterine strips isolated from pregnant (Gestation Day 10) rats to cumulative concentrations of lindane at 10-min intervals was found to depress spontaneous contractions at 10 μM lindane and abolish contractions at 30 μM lindane (Criswell and Loch-Caruso, 1999).…”

Section: Introductionmentioning

confidence: 99%

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Phospholipase-Mediated Inhibition of Spontaneous Oscillatory Uterine Contractions by Lindane in Vitro,

Wang

Loch‐Caruso

2002

Toxicology and Applied Pharmacology

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Although regulation of uterine contractility is fundamental for parturition, mechanisms by which toxicants modify uterine muscle contractions remain poorly understood. In a previous cumulative concentration-response study, 10 μM lindane (γ-hexachlorocyclohexane) reduced contraction force and 30 μM lindane abolished contractions in Gestation Day 10 rat uterine strips when lindane was added to muscle baths at 10-min intervals. Other studies showed that brief (<10 min) exposures to 10-100 μM lindane inhibit gap junctions and activate phospholipase pathways in rat myometrial cells in culture. Consequently, lindane was used as a prototype toxicant with known uterine activity to investigate the hypothesis that activation of a specific phospholipase pathway provides a mechanistic link between inhibition of uterine contraction and inhibition of myometrial gap junctions. Uterine tissue and cells were pretreated with phospholipase pathway inhibitors to evaluate the role of phospholipase pathways in lindane's actions in the uterus. Concentrations of inhibitors were selected based on previous reports of effective concentrations for the enzyme activity and on pilot toxicity studies of the inhibitors on uterine contraction and gap junction communication. To monitor uterine contractions, longitudinal uterine strips were excised from Gestation Day 10 rats and suspended in isometric muscle baths, consistent with previous experiments. Exposure in vitro for 60 min to 10-50 μM lindane, an effective concentration range for the uterine responses of interest, revealed that 30 μM lindane rapidly abolished contractions. Subsequently, uterine strips were pretreated with phospholipase pathway inhibitors and then challenged with 30 μM lindane, the lindane concentration that elicited maximal inhibition of uterine contraction. Pretreatment with 20-50 μM of the phosphatidylinositol-specific phospholipase C inhibitor 1-O-octadecyl-2-O-methyl-sn -glycerol-3-phosphorylcholine (ET-18-OCH 3 ) reversed lindane-induced inhibition of spontaneous uterine contractions. Gap junction intercellular communication was monitored by injecting the fluorescent dye Lucifer yellow into rat myometrial cells grown in culture and assessing dye transfer to adjacent cells using epifluorescence microscopy. Similar to uterine contraction, pretreatment of cell cultures with phospholipase C inhibitors (30 μM ET-18-OCH 3 , 50 μM tricyclodecan-p-yl-xanthogenate · K [D609] or 50 μM tricyclodecan-p-yl-xanthogenate · K or 2-nitro-4-carboxyphenyl-N,Ndophenylcarbamate [NCDC]) partially reversed inhibition of dye transfer by 100 μM lindane, a 4To whom correspondence and reprint requests should be addressed at 1420 Washington Heights, Department of Environmental Health Sciences, School of Public Health, University of Michigan, Ann Arbor, MI 48109-2029. Fax: (734) lindane concentration previously shown to abolish myometrial Lucifer yellow dye transfer under similar culture conditions. In contrast, pretreatment with 20 μM of bromoenol lactone (BEL) to inhibit the calcium-i...

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Organochlorine Pesticides in Specimens from Women Undergoing Spontaneous Abortion, Premature or Full-Term Delivery

Cited by 107 publications

References 8 publications

The stress of being contaminated? Adrenocortical function and reproduction in relation to persistent organic pollutants in female black legged kittiwakes

The stress of being contaminated? Adrenocortical function and reproduction in relation to persistent organic pollutants in female black legged kittiwakes

Evaluation of organochlorine pesticide residues in human serum from an urban and two rural populations in Portugal

Phospholipase-Mediated Inhibition of Spontaneous Oscillatory Uterine Contractions by Lindane in Vitro,

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