Organization, ultrastructure, and development of midgut visceral muscle in larval Aedes aegypti

Bernick, E.P.; Moffett, Stacia B.; Moffett, David F.

doi:10.1016/j.tice.2007.05.003

Cited by 31 publications

(24 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bifurcating circular muscle bands are present in both the anterior and posterior midgut (anterior seen in Fig. 3a), a feature identified in the larval muscularis (Bernick et al, 2007). Bifurcating longitudinal muscle bands, yet another larval feature, were identified in the pupal posterior midgut, approaching the junction with the hindgut (Fig.…”

Section: Gross Morphology and Development Of The Pupal Midgut Muscularismentioning

confidence: 91%

“…Preparation for transmission electron microscopy occurred in a manner quite similar to that occurring in our previous studies on the larval midgut muscularis (Bernick et al, 2007). The major difference involved the infiltration of the liquid SPURRS resin for pupal tissues, which included an overnight incubation of two parts acetone:one part SPURRS not used during previous larval experiments.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

“…aegypti midguts (Bernick et al, 2007). Following preparation, specimens were gold coated on a Technics Hummer V sputter coater (Technics, San Jose, CA).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

“…In Ae. aegypti, the larval midgut muscularis arises by the second larval instar and develops extensively throughout the larval stadia (Bernick et al, 2007). Both the larval and adult midgut muscularis take the form of a grid of circularly and longitudinally oriented bands of muscle (Bernick et al, 2007;Park and Shahabuddin, 2000).…”

Section: Introductionmentioning

confidence: 99%

“…In larvae, grid intersections include regions of band overlap and cruciform fibers with both circularly and longitudinally oriented filaments. Extensive bifurcation of individual muscle bands are present (Bernick et al, 2007). Sharing of filaments between otherwise discrete bands occurs in both larvae and adults (Bernick et al, 2007;Park and Shahabuddin, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Ultrastructure and morphology of midgut visceral muscle in early pupal Aedes aegypti mosquitoes

Bernick

Moffett

2008

Tissue and Cell

Self Cite

View full text Add to dashboard Cite

These studies focus on the pupal Aedes aegypti midgut muscularis for the first 26 h following larvalpupal transition. The midgut muscularis of Ae. aegypti pupae during this first half of the pupal stadium is a grid of both circularly and longitudinally oriented muscle bands, arranged in a manner resembling that of the larvae. While many muscle bands exhibit signs of degeneration during the time period studied, not all bands degrade, nor is this degradation simultaneous. Band deterioration involves destruction of internal elements while the muscle fiber plasma membrane remains intact. Deterioration of contractile elements may involve proteosome-like structures and associated enzymes. Many features of the larval muscularis including cruciform cells, bifurcating circular bands, and bifurcating longitudinal bands of muscle are retained during the time period investigated. Neuromuscular junctions along some muscle bands are retained through at least 16 h into the pupal stadium. The selective nature of muscle fiber degradation, coupled with the retention of larval features and neural input, may allow for limited functionality of the muscularis during metamorphosis. Evidence of sexual dimorphism in the midgut muscularis of male and female Ae. aegypti pupae was not observed during the time period studied.

show abstract

Section: Gross Morphology and Development Of The Pupal Midgut Muscularismentioning

confidence: 91%

Section: Transmission Electron Microscopymentioning

confidence: 99%

“…aegypti midguts (Bernick et al, 2007). Following preparation, specimens were gold coated on a Technics Hummer V sputter coater (Technics, San Jose, CA).…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Ultrastructure and morphology of midgut visceral muscle in early pupal Aedes aegypti mosquitoes

Bernick

Moffett

2008

Tissue and Cell

Self Cite

View full text Add to dashboard Cite

show abstract

Embryonic development of the alimentary canal of the scorpionfly Panorpa obtusa Cheng (Mecoptera: Panorpidae)

Yue

Hua

2013

Microscopy Res & Technique

View full text Add to dashboard Cite

The embryonic origin of the alimentary canal, especially the midgut, is a controversial problem in insects, and it has not been satisfactorily resolved to date. The organogenesis of the digestive system in the embryonic development was observed in the scorpionfly Panorpa obtusa Cheng using light, transmission, and scanning electron microscopy. The embryonic development lasts about 150-160 h at 24°C. The stomodaeum is formed from an invagination in the medioposterior portion of the protocephalon mid-ventrally posterior to the labral segment at 76 h after oviposition. The proctodaeum arises as an invagination from the caudal end of the abdomen at 78 h. Four anal forks are formed from within the opening of proctodaeum. Three pairs of proctodaeal evaginations are formed from the anterior part of the proctodaeum, and eventually developing into Malpighian tubules, thus are of ectodermal origin. The cardiac and pyloric valves develop from stomodaeum and proctodaeum, respectively, and also of ectodermal origin. The midgut epithelium originates from anterior and posterior midgut rudiments in blind ends of the stomodaeum and proctodaeum, and it is of endodermal origin. The two cell-bands (rudiments) cover the yolk ventrally and then dorsally, elongate to each other, and eventually fuse to form the midgut. The midgut formation pattern is briefly discussed in different insects.

show abstract

Larvicidal activity of catechin isolated from Leucas aspera against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)

et al. 2015

View full text Add to dashboard Cite

Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of plant origin my serve as an alternative biocontrol technique in the future. The aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of Leucas aspera on the fourth-instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus. The larvae were exposed to fractions with concentrations ranging from 1.25, 2.25, 5, 10, and 20 ppm and isolated compounds. After 24 h exposure, larval mortality was assessed. Among the eight fractions, four from hexane extractions showed potent larvicidal activity against tested mosquito species at 20 ppm concentration. The isolated compound catechin showed pronounced larvicidal activity at very low concentrations. The LC50 and LC90 values of catechin were 3.05 and 8.25 ppm against Ae. aegypti, 3.44 and 8.89 ppm against An. stephensi, and 3.76 and 9.79 ppm against C. quinquefasciatus, respectively. The isolated compound was subjected to spectral analyses (GC-MS, FTIR, (1)H NMR, and (13)C NMR) to elucidate the structure and to compare with spectral data literature.

show abstract

Organization, ultrastructure, and development of midgut visceral muscle in larval Aedes aegypti

Cited by 31 publications

References 22 publications

Ultrastructure and morphology of midgut visceral muscle in early pupal Aedes aegypti mosquitoes

Ultrastructure and morphology of midgut visceral muscle in early pupal Aedes aegypti mosquitoes

Embryonic development of the alimentary canal of the scorpionfly Panorpa obtusa Cheng (Mecoptera: Panorpidae)

Larvicidal activity of catechin isolated from Leucas aspera against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)

Contact Info

Product

Resources

About