Organization of the Haemophilus ducreyi 35000 chromosome

Hobbs, Marcia M.; Leonardi, Mary Jean; Zaretzky, Franca R.; Wang, Ting Hsien; Kawula, Thomas H.

doi:10.1099/00221287-142-9-2587

Cited by 8 publications

(4 citation statements)

References 21 publications

(7 reference statements)

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“…The sizes of the chromosomes of the A. pleuropneumoniae type and serotype reference strains were determined to range between 2.3 and 2.4 Mb using PFGE analysis of AscI and ApaI digested chromosomal DNA. The size of the A. pleuropneumoniae chromosome is comparable to that of other species of the family Pasteurellaceae which includes Actinobacillus actinomycetemcomitans (2.3 Mb) [21], Haemophilus parain£uenzae (2.34 Mb), Haemophilus in£uenzae (1.83 Mb) [22] and Haemophilus ducreyi (1.76 Mb) [23]. The sizes of the chromosomes of the di¡erent A. pleuropneumoniae strains analyzed vary relatively little compared to other bacterial species.…”

Section: Discussionmentioning

confidence: 73%

Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Chevallier¹

1998

FEMS Microbiology Letters

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The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074 (serotype 1) was determined to be 2404 þ 40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to crossreact serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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Section: Discussionmentioning

confidence: 73%

Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Chevallier¹

1998

FEMS Microbiology Letters

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“…The sizes of the chromosomes of the A. pleuropneumoniae type and serotype reference strains were determined to range between 2.3 and 2.4 Mb using PFGE analysis of Asc I and Apa I digested chromosomal DNA. The size of the A. pleuropneumoniae chromosome is comparable to that of other species of the family Pasteurellaceae which includes Actinobacillus actinomycetemcomitans (2.3 Mb) [21], Haemophilus parainfluenzae (2.34 Mb), Haemophilus influenzae (1.83 Mb) [22] and Haemophilus ducreyi (1.76 Mb) [23]. The sizes of the chromosomes of the different A. pleuropneumoniae strains analyzed vary relatively little compared to other bacterial species.…”

Section: Discussionmentioning

confidence: 74%

Chromosome sizes and phylogenetic relationships between serotypes ofActinobacillus pleuropneumoniae

Chevallier¹,

Dugourd

Tarasiuk

et al. 1998

FEMS Microbiology Letters

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The genome size of Actinobacillus pleuropneumoniae was determined by pulsed field gel electrophoresis of AscI and ApaI digested chromosomal DNA. The genome size of the type strain 4074T (serotype 1) was determined to be 2404 +/- 40 kb. The chromosome sizes for the reference strains of the other serotypes range between 2.3 and 2.4 Mb. The restriction pattern profiles of AscI, ApaI and NheI digested chromosomes showed a high degree of polymorphism among the different serotype reference strains and allowed their discrimination. The analysis of the macrorestriction pattern polymorphism revealed phylogenetic relationships between the different serotype reference strains which reflect to some extent groups of serotypes known to cross-react serologically. In addition, different pulsed fields gel electrophoresis patterns also revealed heterogeneity in the chromosomal structure among different field strains of serotypes 1, 5a, and 5b, while strains of serotype 9 originating from most distant geographical places showed homogeneous ApaI patterns in pulsed field gel electrophoresis.

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“…The rRNA genes and groEL gene exist in multiple copies in most eubacteria, including H. ducreyi 19,33 . Species-speci® c regions of these evolutionarily conserved genes have been successfully used as targets for PCR assays for the detection and differentiation of pathogenic microorganisms 31 .…”

Section: Discussionmentioning

confidence: 99%

Development of a heminested polymerase chain reaction assay for the detection of Haemophilus ducreyi in clinical specimens

et al. 2001

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Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens.

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Organization of the Haemophilus ducreyi 35000 chromosome

Cited by 8 publications

References 21 publications

Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Chromosome sizes and phylogenetic relationships between serotypes ofActinobacillus pleuropneumoniae

Development of a heminested polymerase chain reaction assay for the detection of Haemophilus ducreyi in clinical specimens

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