Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Chevallier, B

doi:10.1016/s0378-1097(98)00033-0

Cited by 7 publications

(10 citation statements)

References 13 publications

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“…bacterial genomes as well as for strain identification [29,32], As shown in Fig. 1, the genome organization of the strain T. ferrooxidans 33020 differs significantly from those of the strains 19859, 21834 and 23270 T (compare lane 3 to the lanes 2, 4, and 5 in the figure).…”

Section: Genomic Analysis Of the T Ferrooxidans Strainsmentioning

confidence: 94%

Complexation of U(VI) with Cells of Thiobacillus ferrooxidans and Thiomonas cuprina of Different Geological Origin

et al. 1999

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Uranium / Thiobacillus ferrooxidans / Bioaccumulation / Time-resolved laser fluorescence spectroscopy SummaryRecently, we demonstrated that the strain Thiobacillus ferrooxidans ATCC 33020 which is recovered from a uranium mine is taxonomically neither closely related to the type strain of the species Thiobacillus ferrooxidans nor to several other strains of this species stemming from copper and coal mines. In the present work, the interaction was studied of the above-mentioned strains with U(VI). We found that the uranium mine isolate 33020 accumulates significantly higher amounts of U(VI) in comparison to the other T. ferrooxidans strains studied. Extraction studies with sulfuric acid released none, and with EDTA only a small fraction (10-42%) of the U(VI) that was accumulated by the bacteria. The main part of the uranium was irreversibly bound to the biomass. Time-resolved laser fluorescence spectroscopy demonstrated the formation of strong inner-sphere complexes of U(VI) with the bacteria. Moreover, the binding strength of the Thiobacillus ferrooxidans complexes corresponds to the accumulation capability of the strains, i.e., the strongest was the complex with the uranium mine isolate 33020.In addition, the interaction was investigated with U(VI) of a bacterial strain recovered from a uranium mine, but belonging to another bacterial species, Thiomonas cuprina. This strain interacts in a different manner with uranium. Its capability to bind U(VI) was higher than those of all T. ferrooxidans strains, but the complexes formed were less stable.

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Section: Genomic Analysis Of the T Ferrooxidans Strainsmentioning

confidence: 94%

Complexation of U(VI) with Cells of Thiobacillus ferrooxidans and Thiomonas cuprina of Different Geological Origin

et al. 1999

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“…After being washed with wash buffer [20 mM tris-HCl (pH 8), 50 mM EDTA and distilled water] and then digested with proteinase K solution overnight at 55°C, each genomic DNA was digested with AscI (New England Bio Labs Inc., Beverly, MA, USA) and ApaI (Takara Bio Inc., Otsu, Japan) for 18 hours. The enzymes best suited for this test through preliminary experiments and previous reports showing distinctive DNA patterns, 6–12 fragments ranging from 11 kb 50 1217 kb in A pleuropneumoniae strains (Chevallier and others 1998). These were separated on 1 per cent SeaKem Gold agarose (Lonza, Rockland, ME, USA) using CHEF MAPPER (Bio-Rad, Hercules, FL, USA).…”

Section: Methodsmentioning

confidence: 99%

Serotypes and antimicrobial resistance patterns of the recent Korean Actinobacillus pleuropneumoniae isolates

Yoo

Shin

et al. 2014

Veterinary Record

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In Korea, the addition of antibiotics to feed was banned in July 2011; since then, reports of outbreaks of porcine pleuropneumonia have increased. Therefore, knowledge of the characteristics of clinical isolates emerging in recent years is needed. This study analysed the serotype and antimicrobial susceptibility of 102 Actinobacillus pleuropneumoniae isolates from pigs with pleuropneumonia in Korea from 2006 to 2010. Serotype 5 was the most frequently isolated but the incidence of serotype 1 significantly increased in 2010. The susceptibility to florfenicol decreased considerably from 2006 to 2010, as did that of a minor percentage of isolates to ampicillin and amoxicillin. Of 11 tetracycline resistance genes, tet (B) was predominant (62 per cent), followed by tet (H) and tet (O). The florfenicol resistance (flo R) gene (34 per cent) and β-lactam antibiotic resistance (bla ROB-1) gene (15 per cent) were also detected. The expression levels of the tetracycline repressor gene and resistance gene were not significantly correlated with the minimum inhibitory concentration values of tetracycline-resistant strains. Interspecies transferability of the resistance genes (flo R, tet (B)/bla ROB-1) was observed with frequencies of 5.7×10 −3 and 3.5×10 −2 , respectively. In analysing DNA patterns using pulsed field gel electrophoresis, there was a slight positive correlation with serotypes but not with isolated location and year. This study acknowledges the emergence of new serotype and antimicrobial resistance of A pleuropneumoniae based on the current situation in Korea. This knowledge may be useful in controlling the disease caused by A pleuropneumoniae.

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“…The top and bottom gels were generated by using short and long running conditions, respectively. The A. pleuropneumoniae serotype reference strains (S1 to S12) are grouped based on their phylogenetic relationship (7). The position of the mapped A. pleuropneumoniae clinical isolate AP76 is indicated by "76."…”

Section: Resultsmentioning

confidence: 99%

“…The different serotypes have been also grouped based either on outer membrane protein profiles (32) or on antigenic similarities (28) which are most likely due to shared species-specific antigens such as lipopolysaccharide or membrane proteins (31). More recently, Chevallier and coworkers assessed the phylogenetic relationship among the different serotype reference strains based on restriction enzyme fragment polymorphisms after pulsed-field gel electrophoresis (PFGE) (7).…”

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confidence: 99%

First Chromosomal Restriction Map ofActinobacillus pleuropneumoniaeand Localization of Putative Virulence-Associated Genes

et al. 1999

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Combined physical and genetic maps of the genomes ofActinobacillus pleuropneumoniae AP76 (serotype 7 clinical isolate) and of A. pleuropneumoniae ATCC 27088 (serotype 1 reference strain) were constructed by using the restriction endonucleases ApaI, AscI, NotI, andSalI. The chromosome sizes as determined by the addition of estimated fragment sizes were 2.4 Mbp, and both maps had a resolution of approximately 100 kbp. The linkages between the ApaI,AscI, NotI, and SalI fragments and their relative positions were determined by (i) fragment excision and redigestion and (ii) partial digests of defined fragments and Southern blot using end-standing probes. The single SalI site within the chromosome of strain A. pleuropneumoniae AP76 was defined as position 1 of the map; for the map of A. pleuropneumoniae ATCC 27088, the corresponding SalI site was chosen. Putative virulence-associated genes (apx,omlA, sodA, tbpBA,ureC, and a repeat element) and housekeeping genes (glyA, metJ, recA, andrhoAP) were positioned on the physical maps and located on the ApaI and NotI fragments of A. pleuropneumoniae serotype reference strains.

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Chromosome sizes and phylogenetic relationships between serotypes of Actinobacillus pleuropneumoniae

Cited by 7 publications

References 13 publications

Complexation of U(VI) with Cells of Thiobacillus ferrooxidans and Thiomonas cuprina of Different Geological Origin

Complexation of U(VI) with Cells of Thiobacillus ferrooxidans and Thiomonas cuprina of Different Geological Origin

Serotypes and antimicrobial resistance patterns of the recent Korean Actinobacillus pleuropneumoniae isolates

First Chromosomal Restriction Map ofActinobacillus pleuropneumoniaeand Localization of Putative Virulence-Associated Genes

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