Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit

Howe, Timothy R.; LaQuier, F W; Barbour, Alan G.

doi:10.1128/iai.54.1.207-212.1986

Cited by 102 publications

(48 citation statements)

References 36 publications

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“…Previous mapping studies had indicated that the ospA gene was at the 5' end of the operon and that ospB was located downstream of ospA (Howe etal., 1986). To our knowledge, ospA and ospB are the first genes sequenced from the genus Borrelia and the data strongly support the suggestion by Howe et al, (1986) that the two genes are organized into an operon. The nucleotide sequence of the ospA-ospB operon and flanking regions together with the deduced amino acid sequence of the ospA and ospB gene products are shown in Fig.…”

Section: Dna Sequence Anaiysis Of the Osp Operonsupporting

confidence: 83%

Molecular analysis of linear plasmid‐encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi

Bergström

Bundoc

Barbour

1989

Molecular Microbiology

305

249

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The ospA and ospB genes encode the major outer membrane proteins of the Lyme disease spirochaete Borrelia burgdorferi. The deduced translation products from the ospA and ospB genes were: (OspA) 273 amino acids long with a molecular weight of 29,334, and (OspB) 296 amino acids long with a molecular weight of 31,739. The two Osp proteins showed a great degree of sequence similarity indicating a recent evolutionary event. Molecular analysis and sequence comparison of OspA and OspB with other proteins revealed a sequence similarity to the signal peptides of prokaryotic lipoproteins. These are the first sequences from Borrelia and provide interesting data on the evolutionary relationship between spirochaetes and other species as well as providing potential for spirochaete diagnostics and vaccines.

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Section: Dna Sequence Anaiysis Of the Osp Operonsupporting

confidence: 83%

Molecular analysis of linear plasmid‐encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi

Bergström

Bundoc

Barbour

1989

Molecular Microbiology

305

249

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“…Extractions of total DNA and plasmid-enriched DNA from the borrelias, standard agarose dectrophoresis, Southern blot analysis, and direct gel hybridization of low-percentage agarose gels were performed as described previously (22,30,48,49). The probes for the ospA and ospB genes of HIB19 and B31 were recombinant plasmids pTRH43 and pTRH46, respectively (50)(51)(52). The probe DNA was labded with ol-[32p]dATP by nick transhtion using a commercial kit (Bethesda Research Laboratories, Gaithersburg, MD).…”

Section: Methodsmentioning

confidence: 99%

Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization.

Sadziene

Rosa

Thompson

et al. 1992

The Journal of Experimental Medicine

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146

152

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SummaryWe used polyclonal antisera and monodonal antibodies (mAbs) to inhibit the growth of clonal populations of two strains of Borrelia burgdorferi, the Lyme disease agent, and thereby select for antibody-resistant mutants, mAbs were directed at the outer membrane proteins, OspA or OspB. Mutants resistant to the growth-inhibiting properties of the antibodies were present in the populations at frequencies ranging from 10 -s to 10 -2. The several escape variants that were examined were of four classes. Class I mutants were resistant to all mAbs; they lacked OspA and OspB and the linear plasmid that encodes them. Two other proteins were expressed in larger amounts in class I mutants; mAbs to these proteins inhibited the mutant but not the wild-type cells. Class II mutants were resistant to some but riot all mAbs; they had truncated OspA and/or OspB proteins. Class III mutants were resistant only to the selecting mAb; they had full-length Osp proteins that were not bound by the selecting antibody in Western blots. In two class III mutants resistant to different anti-OspA mAbs, missense mutations were demonstrated in the ospA genes. Class IV mutants were likewise resistant only to selecting antibody, but in this case the selecting antibody still bound in Western blots.

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“…We and others demonstrated previously that a single copy of this locus containing these two genes is located on the spirochete's chromosome [1,10]. The genes encoding OspA and OspB of B. burgdorferi are encoded by a single transcriptional unit [11,12]; however, this locus is located on the 49-53-kb linear plasmid having covalently closed ends [13]. The ospD gene is also located on a different linear plasmid of approximately 38 kb [9] while the ospC gene has been mapped recently to a 26-27-kb circular plasmid [14,15].…”

Section: Consensus Sequences (Cons) Are Also Shown Dots Indicate Gapmentioning

confidence: 96%

Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen

Simpson

1994

FEMS Microbiology Letters

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The P39 antigen is a specific, highly conserved, and immunogenic protein of Lyme disease spirochetes, Borrelia burgdorferi sensu lato. The nucleotide sequence of the gene encoding this protein was determined and found to be the first of two tandemly arranged open reading frames located on the spirochete's chromosome. These two open reading frames were designated bmpA for the gene encoding P39 and bmpB for the gene encoding the putative protein ORF2 encoded by the second open reading frame. The nucleic acid sequence identity for the two open reading frames was 62% while their deduced amino acid sequences were 52% identical. Comparison to sequence data bases demonstrated that the deduced amino acid sequences of both P39 and ORF2 were homologous to TmpC, a putative outer or cytoplasmic membrane lipoprotein of the syphilis spirochete, Treponema pallidum.

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Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit

Cited by 102 publications

References 36 publications

Molecular analysis of linear plasmid‐encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi

Molecular analysis of linear plasmid‐encoded major surface proteins, OspA and OspB, of the Lyme disease spirochaete Borrelia burgdorferi

Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization.

Nucleotide sequence and analysis of the gene in Borrelia burgdorferi encoding the immunogenic P39 antigen

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