“…The increase in the value of I 384 /I 373 ratio indicates the increase in the nonpolarity of the system [21,25,37] . By investigating the change in I 384 /I 373 ratio, the formation of the aggregates can be revealed [21,25,37]. by the sharp change in the polarity parameter of pyrene without BSA as observed by us earlier [25,37] and excludes the formation of micelles through premicellar aggregates formed in a step-wise manner [20].…”