Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus

Gorbulev, Valentin; Akhundova, Aida; Grzeschik, K.-H.; Fahrenholz, Falk

doi:10.1016/0014-5793(94)80150-9

Cited by 36 publications

(29 citation statements)

References 15 publications

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“…2). The gene encoded for human homologue of BRS-3 was reported subsequently (Fathi et al, 1993;Gorbulev et al, 1994). As in the guinea-pig, BRS-3 is expressed in human uterus.…”

Section: Cloning Of Bombesin-like Peptide Receptor Subtype-3 (Brs-3)mentioning

confidence: 96%

Development and function of bombesin-like peptides and their receptors

Ohki‐Hamazaki

Iwabuchi

Maekawa³

2005

Int. J. Dev. Biol.

151

123

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Amphibian bombesin and its related peptides consist a family of neuropeptides in many vertebrate species. Bombesin and two major bombesin-like peptide in mammals, gastrinreleasing peptide (GRP) and neuromedin B (NMB), have been shown to elicit various physiological effects. These include inhibition of feeding, smooth muscle contraction, exocrine and endocrine secretions, thermoregulation, blood pressure and sucrose regulations and cell growth. Receptors for GRP and NMB (GRP-R and NMB-R), as well as third subtype of bombesin-like peptide receptor (BRS-3) have been cloned. These receptors are G-protein-coupled receptors and are expressed in various brain regions and in the digestive tract. In this paper, we will summarize studies on these peptides and their receptors, with special reference to research using gene-knockout mice. These studies clearly demonstrated the role of three receptors in vivo and in vitro. We will also discuss the phylogeny of these receptors. KEY WORDS: gastrin-releasing peptide, neuromedin B, bombesin-like peptide receptor subtype-3 (BRS-3)Int.

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“…2). The gene encoded for human homologue of BRS-3 was reported subsequently (Fathi et al, 1993;Gorbulev et al, 1994). As in the guinea-pig, BRS-3 is expressed in human uterus.…”

Section: Cloning Of Bombesin-like Peptide Receptor Subtype-3 (Brs-3)mentioning

confidence: 96%

Development and function of bombesin-like peptides and their receptors

Ohki‐Hamazaki

Iwabuchi

Maekawa³

2005

Int. J. Dev. Biol.

151

123

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“…The human BB 3 receptor gene is localized at human chromosome Xq25 and in the mouse on chromosome XA7.1-7.2 (Fathi et al, 1993b;Gorbulev et al, 1994;Weber et al, 1998). The human BB 3 receptor gene (Fathi et al, 1993b;Gorbulev et al, 1994;Weber et al, 1998) contained two introns and three exons similar to the sheep (Whitley et al, 1999), rhesus (Sano et al, 2004), mouse (Ohki-Hamazaki et al, 1997a), and rat BB 3 receptor genes (Liu et al, 2002).…”

Section: Bb 3 Receptor Genomic Organizationmentioning

confidence: 99%

“…The human BB 3 receptor gene (Fathi et al, 1993b;Gorbulev et al, 1994;Weber et al, 1998) contained two introns and three exons similar to the sheep (Whitley et al, 1999), rhesus (Sano et al, 2004), mouse (Ohki-Hamazaki et al, 1997a), and rat BB 3 receptor genes (Liu et al, 2002). In the mouse the BB 3 receptor gene spanned more than 5 kb with exon 1 of the BB 3 gene separated from exon 2 by 1.6 kb and this in turn separated from exon 3 by 1.6 kb .…”

Section: Bb 3 Receptor Genomic Organizationmentioning

confidence: 99%

International Union of Pharmacology. LXVIII. Mammalian Bombesin Receptors: Nomenclature, Distribution, Pharmacology, Signaling, and Functions in Normal and Disease States

Jensen

Battey

Spindel³

et al. 2007

Pharmacol Rev

475

720

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“…Bombesin receptor subtype 3 (BRS-3) mRNA has been detected using reverse transcription PCR in the pregnant myometrium of guinea pigs (Gorbulev et al 1992) and women (Fathi et al 1993, Gorbulev et al 1994 but not in the non-pregnant myometrium of these species. Our own studies found BRS-3 mRNA to be expressed in the pregnant endometrium, myometrium, amnion, chorion and placenta isolated from three women at term delivery by caesarean section .…”

Section: Figurementioning

confidence: 99%

Temporal expression and cellular localization of a gastrin-releasing peptide-related gene in ovine uterus during the oestrous cycle and pregnancy

Whitley

Shulkes²,

Salamonsen³

et al. 1998

Journal of Endocrinology

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Synthesis of both mRNA and peptide for gastrin-releasing peptide (GRP) has been demonstrated in the pregnant endometrium of sheep and women. However, it is not known whether GRP is synthesized in the sheep uterus during the oestrous cycle. Furthermore the cellular site of GRP mRNA synthesis in the uterus has not been determined. Therefore we examined the synthesis of GRP and determined the cellular location of GRP peptide and mRNA in sheep uterus taken at different times during the oestrous cycle (duration 17 days) and pregnancy (duration 145 days). Northern blot analysis of RNA isolated from ovine endometrium revealed low or no GRP mRNA at days 4, 10, 12 and 14 of the oestrous cycle and a 24-fold rise in GRP mRNA (normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA) between days 14 and 16. A similar pattern was observed during early pregnancy, with a 12-fold rise in GRP mRNA-:GAPDH mRNA between days 17 and 20 of pregnancy. Levels of GRP peptide were determined by RIA and found to be low in endometrium isolated at days 4, 10, 12 and 14 of the oestrous cycle (1·0-1·6 pmol/g) and 4 to 5-fold higher at day 16. In situ hybridization localized GRP synthesis to the epithelial cells of the uterine glands at day 16 of the oestrous cycle and at days 17, 20, 40 and 50 of pregnancy. At day 140 of pregnancy diffuse hybridization to cells of the myometrium was also observed. Immunohistochemistry localized GRP peptide to the apical cytoplasm of uterine glandular epithelial cells at day 16 of the oestrous cycle. For samples obtained at day 20 of pregnancy, the area surrounding the glands also showed moderately strong staining. Further staining in the glandular lumen and the stromal tissue surrounding the glands was apparent at day 140 of pregnancy. No GRP immunoreactivity could be detected in the peripheral plasma during the oestrous cycle or the first 20 days of pregnancy. Sizing chromatography of GRP immunoreactivity extracted from endometrial tissue taken at day 10 of the oestrous cycle revealed two peaks that co-eluted with GRP(1-27) and . However, during luteolysis and oestrus the major peak of GRP immunoreactivity extracted from endometrial tissue was larger than GRP(1-27) and similar to that seen previously in the gravid ovine endometrium. These studies demonstrate that a peptide similar to, but larger than, GRP is a major product of the glandular epithelium of the ovine uterus during the luteal regression phase of the oestrous cycle and post-blastocyst implantation in pregnancy and provide further evidence that GRP-related peptides have important regulatory roles in uterine function.

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Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus

Cited by 36 publications

References 15 publications

Development and function of bombesin-like peptides and their receptors

Development and function of bombesin-like peptides and their receptors

International Union of Pharmacology. LXVIII. Mammalian Bombesin Receptors: Nomenclature, Distribution, Pharmacology, Signaling, and Functions in Normal and Disease States

Temporal expression and cellular localization of a gastrin-releasing peptide-related gene in ovine uterus during the oestrous cycle and pregnancy

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